Time bound changes (in 24h) in human sperm motility and level of calcium and magnesium in seminal plasma

AbstractA detailed sperm motility study for 24h after collection was done. The level of calcium and magnesium in seminal plasma during this period was also seen to understand the role of these electrolytes on sperm motility.Good care was taken in selection of subjects (young and healthy), collection and pre-physical analysis of sample. Subjects (healthy, 18–25years) were to maintain abstinence (2–5days). Collection of sample into wide mouthed container provided was done next to laboratory by masturbation at 8am±30m. The total study was done in one month period. Samples were studied at ½, 1, 2, 4, 8, 12 and 24h time for percentage of (1) total sperm motility, (2) active and progressive and (3) non-progressive motility and seminal plasma calcium and magnesium. Total sperm motility deteriorated to 50% in 8h whereas active and progressive type reduced to 50% in 4h. At 24h total sperm motility was 9.8% and active and progressive motility 3.0%.Level of calcium (27.2mg/dl) and magnesium (13.54mg/dl) in seminal plasma did not show any significant changes during study period from that of at ½h. The study concluded that electrolytes under study were not responsible for the decrease in motility during study period

Gold in semen: Level in seminal

The study was conducted to understand the amount of gold in semen of normal and different infertile conditions. Gold was estimated in normal (n38) and pathological conditions (n86) by employing Atomic Absorption Spectrophotometer. Gold level observed in seminal plasma was as follows: in normozoospermia (n38) – 17.0 μg% ± 9.63; oligozoospermia (n23) – 13.0 μg% ± 0.73; oligoasthenozoospermia (n44) – 20.5 μg%5 ± .58 and in azoospermia (n19) 21.5 μg% ± 7.52. Gold level in sediment (spermatozoa) of normal was almost same as observed in its seminal plasma whereas in oligozoospermia and oligoasthenozoospermia the level was low