\u418\u434\u435\u43d\u442\u438\u444\u438\u43a\u430\u446\u438\u44f \u41d\u430 \u425\u430\u43b\u43e\u444\u438\u43b\u43d\u438 \u411\u430\u43a\u442\u435\u440\u438\u438 \u41e\u442 \u420\u438\u431\u435\u43d \u421\u43e\u441 (Nam-Pla) \u412 \u422\u430\u439\u43b\u430\u43d\u434

Four strains of Gram-negative, rod-shaped, moderately halophilic bacteria, Group A, and eleven strains of strictly aerobic, extremely halophilic rods (10 strains, Group B) and coccoid (1 strain, Group C) were isolated from fish sauce fermentation (nam-pla) in Thailand. The 16S rRNA gene sequence analyses of the representative strains indicated that DS26-2 (Group A), HDS2-5 (Group B), and HRF6 (Group C), were closely related to Chromohalobacter salexigens KCTC 12941T, Halobacteirum salinarum JCM 8978T, and Halococcus saccharolyticus JCM 8878T with 99.3, 99.9, and 99.0 % similarity, respectively. Group A strains were identified as C. salexigens, Group B as H. salinarum, and Group C strain was H. saccharolyticus based on their DNA-DNA relatedness. Group A strains grew in 3\u201325 % (w/v) NaCl. Ubiquinone with nine isoprene units (Q-9) was a major component. The DNA G+C contents ranged from 63.1 to 64.2 mol %. Group B and Group C strains grew optimally in the presence of 25-30 % NaCl. The tested strains of Group B contained major menaquinone with eight isoprene units (MK-8). DNA G+C contents ranged from 63.3 to 64.7 mol %. Group C strain had MK-8(H2) as a predominant menaquinone. The DNA G+C content was 63.2 mol %.\u41e\u442 \u444\u435\u440\u43c\u435\u43d\u442\u438\u440\u430\u43b \u440\u438\u431\u435\u43d \u441\u43e\u441 (nam-pla) \u432 \u422\u430\u439\u43b\u430\u43d\u434 \u441\u430 \u438\u437\u43e\u43b\u438\u440\u430\u43d\u438 \u447\u435\u442\u438\u440\u438 \u449\u430\u43c\u430 \u413\u440\u430\u43c-\u43e\u442\u440\u438\u446\u430\u442\u435\u43b\u43d\u438 \u431\u430\u43a\u442\u435\u440\u438\u438, \u441 \u43f\u440\u44a\u447\u43a\u43e\u432\u43e\u434\u43d\u430 \u444\u43e\u440\u43c\u430, \u443\u43c\u435\u440\u435\u43d\u43e \u445\u430\u43b\u43e- \u444\u438\u43b\u43d\u438 (\u413\u440\u443\u43f\u430 \u410) \u438 \u435\u434\u438\u43d\u430\u434\u435\u441\u435\u442 \u449\u430\u43c\u430 \u43d\u430 \u43e\u431\u43b\u438\u433\u430\u442\u43d\u43e \u430\u435\u440\u43e\u431\u43d\u438, \u43a\u440\u430\u439\u43d\u43e \u445\u430\u43b\u43e- \u444\u438\u43b\u43d\u438 \u431\u430\u43a\u442\u435\u440\u438\u438, \u43e\u442 \u43a\u43e\u438\u442\u43e 10 \u449\u430\u43c\u430 \u43f\u440\u44a\u447\u43a\u43e\u432\u438\u434\u43d\u438 (\u413\u440\u443\u43f\u430 \u412) \u438 \u435\u434\u438\u43d \u43a\u43e\u43a\u43e\u438\u434\u435\u43d \u449\u430\u43c (\u413\u440\u443\u43f\u430 \u421). \u421\u435\u43a\u432\u435\u43d\u446\u438\u43e\u43d\u43d\u438\u44f\u442 \u430\u43d\u430\u43b\u438\u437 \u43d\u430 16S \u440\u420\u41d\u41a \u43d\u430 \u43f\u440\u435\u434\u441\u442\u430\u432\u438\u442\u435\u43b\u43d\u438 \u449\u430\u43c\u43e\u432\u435, \u43e\u431\u43e\u437\u43d\u430\u447\u435\u43d\u438 \u43a\u430\u442\u43e DS26-2 (\u413\u440\u443\u43f\u430 A), HDS2-5 (\u413\u440\u443\u43f\u430 B) \u438 HRF6 (\u413\u440\u443\u43f\u430 C), \u43f\u43e\u43a\u430\u437\u432\u430, \u447\u435 \u442\u435 \u441\u430 \u442\u44f\u441\u43d\u43e \u440\u43e\u434\u441\u442\u432\u435\u43d\u438 \u43d\u430 Chromohalobacter salexigens KCTC 12941T, Halobacteirum salinarum JCM 8978T \u438 Halococcus saccharolyticus JCM 8878T, \u441\u44a\u43e\u442\u432\u435\u442\u43d\u43e \u441\u44a\u441 \u441\u445\u43e\u434\u441\u442\u432\u43e 99.3, 99.9 \u438 99.0 %. \u41d\u430 \u431\u430\u437\u430 \u43d\u430 \u414\u41d\u41a-\u414\u41d\u41a \u440\u43e\u434- \u441\u442\u432\u43e\u442\u43e \u449\u430\u43c\u43e\u432\u435\u442\u435 \u43e\u442 \u413\u440\u443\u43f\u430 \u410 \u441\u435 \u438\u434\u435\u43d\u442\u438\u444\u438\u446\u438\u440\u430\u442 \u43a\u430\u442\u43e C. salexigens, \u43e\u442 \u413\u440\u443\u43f\u430 \u412 \u43a\u430\u442\u43e H. salinarum, \u430 \u43e\u442 \u413\u440\u443\u43f\u430 \u421 \u43a\u430\u442\u43e H. saccharolyticus. \u429\u430\u43c\u43e\u432\u435\u442\u435 \u43e\u442 \u413\u440\u443\u43f\u430 \u410 \u440\u430\u441\u442\u430\u442 \u432 3\u201325 % (w/v) NaCl. \u413\u43b\u430\u432\u43d\u438\u44f\u442 \u43a\u43e\u43c\u43f\u43e\u43d\u435\u43d\u442 \u435 \u443\u431\u438\u445\u438\u43d\u43e\u43d\u44a\u442 \u441 \u434\u435\u432\u435\u442 \u438\u437\u43e\u43f\u440\u435\u43d\u43e\u432\u438 \u435\u434\u438\u43d\u438\u446\u438 (Q-9), \u430 \u441\u44a\u434\u44a\u440\u436\u430\u43d\u438\u435\u442\u43e \u43d\u430 \u414\u41d\u41a G+C \u432\u430\u440\u438\u440\u430 \u43e\u442 63.1 \u434\u43e 64.2 mol %. \u429\u430\u43c\u43e\u432\u435\u442\u435 \u43e\u442 \u413\u440\u443\u43f\u430 \u412 \u438 \u413\u440\u443\u43f\u430 \u421 \u440\u430\u441\u442\u430\u442 \u43e\u43f\u442\u438\u43c\u430\u43b\u43d\u43e \u432 \u43f\u440\u438\u441\u44a\u441\u442\u432\u438\u435\u442\u43e \u43d\u430 25-30 % NaCl. \u418\u437\u441\u43b\u435\u434\u432\u430\u43d\u438\u442\u435 \u449\u430\u43c\u43e\u432\u435 \u43e\u442 \u413\u440\u443\u43f\u430 \u412 \u441\u44a\u434\u44a\u440\u436\u430\u442 \u43e\u441\u43d\u43e\u432\u43d\u43e \u43c\u435\u43d\u430\u445\u438\u43d\u43e\u43d \u441 \u434\u435\u432\u435\u442 \u438\u437\u43e\u43f\u440\u435\u43d\u43e\u432\u438 \u435\u434\u438\u43d\u438\u446\u438 (MK-8). \u421\u44a\u434\u44a\u440\u436\u430\u43d\u438\u435\u442\u43e \u438\u43c \u430 \u414\u41d\u41a G+C \u435 \u43e\u442 63.3 to 64.7 mol %. \u429\u430\u43c\u44a\u442 \u43e\u442 \u413\u440\u443\u43f\u430 \u421 \u438\u43c\u430 MK-8(H2) \u43a\u430\u442\u43e \u43f\u440\u435\u43e- \u431\u43b\u430\u434\u430\u432\u430\u449 \u43c\u435\u43d\u430\u445\u438\u43d\u43e\u43d, \u430 \u441\u44a\u434\u44a\u440\u436\u430\u43d\u438\u435\u442\u43e \u43d\u430 \u414\u41d\u41a G+C \u435 63.2 mol %

Идентификация На Халофилни Бактерии От Рибен Сос (Nam-Pla) В Тайланд

Four strains of Gram-negative, rod-shaped, moderately halophilic bacteria, Group A, and eleven strains of strictly aerobic, extremely halophilic rods (10 strains, Group B) and coccoid (1 strain, Group C) were isolated from fish sauce fermentation (nam-pla) in Thailand. The 16S rRNA gene sequence analyses of the representative strains indicated that DS26-2 (Group A), HDS2-5 (Group B), and HRF6 (Group C), were closely related to Chromohalobacter salexigens KCTC 12941T, Halobacteirum salinarum JCM 8978T, and Halococcus saccharolyticus JCM 8878T with 99.3, 99.9, and 99.0 % similarity, respectively. Group A strains were identified as C. salexigens, Group B as H. salinarum, and Group C strain was H. saccharolyticus based on their DNA-DNA relatedness. Group A strains grew in 3–25 % (w/v) NaCl. Ubiquinone with nine isoprene units (Q-9) was a major component. The DNA G+C contents ranged from 63.1 to 64.2 mol %. Group B and Group C strains grew optimally in the presence of 25-30 % NaCl. The tested strains of Group B contained major menaquinone with eight isoprene units (MK-8). DNA G+C contents ranged from 63.3 to 64.7 mol %. Group C strain had MK-8(H2) as a predominant menaquinone. The DNA G+C content was 63.2 mol %.От ферментирал рибен сос (nam-pla) в Тайланд са изолирани четири щама Грам-отрицателни бактерии, с пръчководна форма, умерено хало- филни (Група А) и единадесет щама на облигатно аеробни, крайно хало- филни бактерии, от които 10 щама пръчковидни (Група В) и един кокоиден щам (Група С). Секвенционният анализ на 16S рРНК на представителни щамове, обозначени като DS26-2 (Група A), HDS2-5 (Група B) и HRF6 (Група C), показва, че те са тясно родствени на Chromohalobacter salexigens KCTC 12941T, Halobacteirum salinarum JCM 8978T и Halococcus saccharolyticus JCM 8878T, съответно със сходство 99.3, 99.9 и 99.0 %. На база на ДНК-ДНК род- ството щамовете от Група А се идентифицират като C. salexigens, от Група В като H. salinarum, а от Група С като H. saccharolyticus. Щамовете от Група А растат в 3–25 % (w/v) NaCl. Главният компонент е убихинонът с девет изопренови единици (Q-9), а съдържанието на ДНК G+C варира от 63.1 до 64.2 mol %. Щамовете от Група В и Група С растат оптимално в присъствието на 25-30 % NaCl. Изследваните щамове от Група В съдържат основно менахинон с девет изопренови единици (MK-8). Съдържанието им а ДНК G+C е от 63.3 to 64.7 mol %. Щамът от Група С има MK-8(H2) като прео- бладаващ менахинон, а съдържанието на ДНК G+C е 63.2 mol %