4,838 research outputs found
The C Terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit
Ku is a heterodimeric protein with double-stranded DNA end-binding activity that operates in the process of nonhomologous end joining. Ku is thought to target the DNA-dependent protein kinase (DNA-PK) complex to the DNA and, when DNA bound, can interact and activate the DNA-PK catalytic subunit (DNA-PKcs). We have carried out a 3′ deletion analysis of Ku80, the larger subunit of Ku, and shown that the C-terminal 178 amino acid residues are dispensable for DNA end-binding activity but are required for efficient interaction of Ku with DNA-PKcs. Cells expressing Ku80 proteins that lack the terminal 178 residues have low DNA-PK activity, are radiation sensitive, and can recombine the signal junctions but not the coding junctions during V(D)J recombination. These cells have therefore acquired the phenotype of mouse SCID cells despite expressing DNA-PKcs protein, suggesting that an interaction between DNA-PKcs and Ku, involving the C-terminal region of Ku80, is required for DNA double-strand break rejoining and coding but not signal joint formation. To gain further insight into important domains in Ku80, we report a point mutational change in Ku80 in the defective xrs-2 cell line. This residue is conserved among species and lies outside of the previously reported Ku70-Ku80 interaction domain. The mutational change nonetheless abrogates the Ku70-Ku80 interaction and DNA end-binding activity
Inadequacies in the conventional treatment of the radiation field of moving sources
There is a fundamental difference between the classical expression for the
retarded electromagnetic potential and the corresponding retarded solution of
the wave equation that governs the electromagnetic field. While the boundary
contribution to the retarded solution for the {\em potential} can always be
rendered equal to zero by means of a gauge transformation that preserves the
Lorenz condition, the boundary contribution to the retarded solution of the
wave equation governing the {\em field} may be neglected only if it diminishes
with distance faster than the contribution of the source density in the far
zone. In the case of a source whose distribution pattern both rotates and
travels faster than light {\em in vacuo}, as realized in recent experiments,
the boundary term in the retarded solution governing the field is by a factor
of the order of {\em larger} than the source term of this solution in
the limit that the distance of the boundary from the source tends to
infinity. This result is consistent with the prediction of the retarded
potential that part of the radiation field generated by a rotating superluminal
source decays as , instead of , a prediction that is
confirmed experimentally. More importantly, it pinpoints the reason why an
argument based on a solution of the wave equation governing the field in which
the boundary term is neglected (such as appears in the published literature)
misses the nonspherical decay of the field
Rodent outbreaks and extreme weather events: a southeast Asian perspective
Singleton, G.R., Htwe, N.M., Nelson, A.D
The angular dependent magnetoresistance in alpha-(BEDT-TTF)_2KHg(SCN)_4
In spite of extensive experimental studies of the angular dependent
magnetoresistance (ADMR) of the low temperature phase (LTP) of
alpha-(BEDT-TTF)_2KHg(SCN)_4 about a decade ago, the nature of LTP remains
elusive. Here we present a new study of ADMR of LTP in alpha-(ET)_2 salts
assuming that LTP is unconventional charge density wave (UCDW). In the presence
of magnetic field the quasiparticle spectrum in UCDW is quantized, which gives
rise to striking ADMR in UCDW. The present model appears to account for many
existing ADMR data of alpha-(BEDT-TTF)_2KHg(SCN)_4 remarkably well.Comment: 6 pages, 4 figure
Wormholes and Flux Tubes in 5D Kaluza-Klein Theory
In this paper spherically symmetric solutions to 5D Kaluza-Klein theory, with
``electric'' and/or ``magnetic'' fields are investigated. It is shown that the
global structure of the spacetime depends on the relation between the
``electrical'' and ``magnetic'' Kaluza-Klein fields. For small ``magnetic''
field we find a wormhole-like solution. As the strength of the ``magnetic''
field is increased relative to the strength of the ``electrical'' field, the
wormhole-like solution evolves into a finite or infinite flux tube depending on
the strengths of the two fields. For the large ``electric'' field case we
conjecture that this solution can be considered as the mouth of a wormhole,
with the , and components of the metric acting as
the source of the exotic matter necessary for the formation of the wormhole's
mouth. For the large ``magnetic'' field case a 5D flux tube forms, which is
similar to the flux tube between two monopoles in Type-II superconductors, or
the hypothesized color field flux tube between two quarks in the QCD vacuum.Comment: 12 pages, 5 eps.figures, REVTEX, Discussion about null surfaces
ammended. References added. To be published in PR
Complex Lagrangians and phantom cosmology
Motivated by the generalization of quantum theory for the case of
non-Hermitian Hamiltonians with PT symmetry, we show how a classical
cosmological model describes a smooth transition from ordinary dark energy to
the phantom one. The model is based on a classical complex Lagrangian of a
scalar field. Specific symmetry properties analogous to PT in non-Hermitian
quantum mechanics lead to purely real equation of motion.Comment: 11 pages, to be published in J.Phys.A, refs. adde
Are rodent population eruptions in southeast Asia associated with quantity or quality of food?
Htwe, N.M., Singleton, G.R., Sluydts, V., Hinds, L.A
Charge-density Waves Survive the Pauli Paramagnetic Limit
Measurements of the resistance of single crystals of (Per)Au(mnt)
have been made at magnetic fields of up to 45 T, exceeding the Pauli
paramagnetic limit of T. The continued presence of
non-linear charge-density wave electrodynamics at T unambiguously
establishes the survival of the charge-density wave state above the Pauli
paramagnetic limit, and the likely emergence of an inhomogeneous phase
analogous to that anticipated to occur in superconductors.Comment: 4 pages, three figure
Does Magnetic Charge Imply a Massive Photon ?
In Abelian theories of monopoles the magnetic charge is required to be
enormous. Using the electric-magnetic duality of electromagnetism it is argued
that the existence of such a large, non-perturbative magnetic coupling should
lead to a phase transition where magnetic charge is permanently confined and
the photon becomes massive. The apparent masslessness of the photon could then
be used as an argument against the existence of such a large, non-perturbative
magnetic charge. Finally it is shown that even in the presence of this
dynamical mass generation the Cabbibo-Ferrari formulation of magnetic charge
gives a consistent theory.Comment: 10 pages LaTe
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