17 research outputs found
2D Geo-electrical imaging for shallow depth investigation in Doon Valley Sub-Himalaya, Uttarakhand, India
Inter-comparison of model, satellite and in situ tropical cyclone heat potential in the North Indian Ocean
Investigation on phase formation of Sr2SnO4 and effect of La-doping on its structural and optical properties
Evaluation of the Position of Mental Foramen for Clinical and Forensic Significance in terms of Gender in Dentate Subjects by Digital Panoramic Radiographs
Design of pH sensitive low-cost adsorbent from the exudate of Lannea coromandelica (Houtt) for remediation of Malachite Green dye from aqueous solution
MicroRNA Expression Profile in Human Macrophages in Response to Leishmania major Infection
International audienceBACKGROUND:Leishmania (L.) are intracellular protozoan parasites able to survive and replicate in the hostile phagolysosomal environment of infected macrophages. They cause leishmaniasis, a heterogeneous group of worldwide-distributed affections, representing a paradigm of neglected diseases that are mainly embedded in impoverished populations. To establish successful infection and ensure their own survival, Leishmania have developed sophisticated strategies to subvert the host macrophage responses. Despite a wealth of gained crucial information, these strategies still remain poorly understood. MicroRNAs (miRNAs), an evolutionarily conserved class of endogenous 22-nucleotide non-coding RNAs, are described to participate in the regulation of almost every cellular process investigated so far. They regulate the expression of target genes both at the levels of mRNA stability and translation; changes in their expression have a profound effect on their target transcripts.METHODOLOGY/PRINCIPAL FINDINGS:We report in this study a comprehensive analysis of miRNA expression profiles in L. major-infected human primary macrophages of three healthy donors assessed at different time-points post-infection (three to 24 h). We show that expression of 64 out of 365 analyzed miRNAs was consistently deregulated upon infection with the same trends in all donors. Among these, several are known to be induced by TLR-dependent responses. GO enrichment analysis of experimentally validated miRNA-targeted genes revealed that several pathways and molecular functions were disturbed upon parasite infection. Finally, following parasite infection, miR-210 abundance was enhanced in HIF-1α-dependent manner, though it did not contribute to inhibiting anti-apoptotic pathways through pro-apoptotic caspase-3 regulation.CONCLUSIONS/SIGNIFICANCE:Our data suggest that alteration in miRNA levels likely plays an important role in regulating macrophage functions following L. major infection. These results could contribute to better understanding of the dynamics of gene expression in host cells during leishmaniasis