3,957 research outputs found
Orthotopic liver transplantation in U.S. veterans under primary tacrolimus immunosupression.
The evolution and refinement of surgical techniques, per ioperative patient care, and immunosuppression hav~ estab~ished orthoto~ic li~er transplantation (OLTX) as a ~ighly successful therapeutic modality for patients wrth end-stage hver disease. In February 1989,Tacrohmus (Prograf®, formerly FK 506)was first used successfully at the University of Pittsburgh Medical Center to treat patients with rejection refractory to cyclosporine-based immunosuppression." Clinical trials utilizing Tacrolimus in solid organ transplantation followed, and in April of 1994 it was approved for use by the Food and Drug Administration
Analysis of genetic diversity of muga silkworm (Antheraea assamensis, Helfer; Lepidoptera : Saturniidae) using RAPD-based molecular markers
Eleven populations of muga silkworm, Antheraea assamensis Helfer, the golden silk yarn producer of northeast India, was subjected to RAPD marker analysis in order to assess its genetic diversity. The genomic DNA extracted from muga silkworms were analysed using 50 random primers among which 36 polymorphic primers generated 309 bands. RAPD profile of the isolated DNA revealed a high level of genetic polymorphism. The average amplicons per primer was found to be 8.58, and 94.82% amplicons were polymorphic. Cluster analysis based on Jaccard’s similarity coefficients resulted in the formation of two main clusters with one population on one cluster and the remaining on the other cluster.Jaccard’s similarity coefficients ranged from 0.122 to 0.863 indicating a high level of genetic diversity within muga silkworm collection. The study concluded that, although there lays little morphological differences among the collected muga silkworm populations, the populations are highly polymorphic which might have enabled the silkworm to survive under a restricted geographical location, that is north east region of India only but under diverse climatic conditions for a long period. This study maybe useful in identifying diverse genetic stocks of A. assamensis, which may be conserved on a priority basis
A high incidence of native portal vein thrombosis in veterans undergoing liver transplantation
The incidence of native portal vein thrombosis (PVT) in liver transplant recipients has been reported to range from 2.1 to 13.8%. We have identified an inordinately high incidence of PVT in a consecutive series of U.S. veterans receiving liver transplants. Between October 1989 and February 1994, 88 consecutive U.S. veterans received 99 orthotopic liver transplants under primary Tacrolimus (Prograf, formerly FK506) based immunosuppression. A number of clinical features were examined in an effort to identify risk factors for PVT and outcome was compared to patients without PVT. Native PVT was present in 23/88 (26%) patients. All of these patients were male U.S. veterans with a mean age of 47 years. When compared to the 65 patients without PVT, we found no significant difference with respect to underlying liver disease, age, Childs-Pugh score (mean = 12), UNOS status as defined prior to April 1995 (95% UNOS 3 or 4), previous abdominal surgery, or liver volume. Median blood loss for patients with PVT (21 units of packed red blood cells) was greater than for those without PVT (14 units, P = 0.04). Portal thrombectomy was performed in 11 patients, 11 patients required mesoportal jump grafts, and 1 patient had an interposition graft. Standard veno-venous bypass was used in 10 patients with single bypass utilized for the remainder. Actuarial patient survival for all patients at 1, 2, and 4 years was 88, 85, and 79%, respectively. There was no significant difference in patients with or without PVT. Patients with PVT had poorer graft survival than patients without PVT (86% vs 65%, 1 year; 81% vs 65%, 2 years; 81% vs 61%, 4 years; P = 0.03); however, this was not related to technical problems with the portal venous inflow. PVT occurred in 26% of U.S. veterans undergoing liver transplantation. These patients bad significantly higher operative blood loss and poorer graft survival. The high incidence of postnecrotic cirrhosis in a predominantly male group of patients with advanced disease, as is evident by the high mean Childs-Pugh score and UNOS status, perhaps accounts for our observations
Anti-inflammatory Activity of Matricaria recutita L. against Acute and Chronic Inflammatory Models
Chamomile (Matricaria recutita L.) is most popular used medicinal plant and extensively consumed as a tea and food. We investigated the effects of Matricaria recutita L. (MR) in acute and chronic inflammatory conditions. The anti-inflammatory activity of Matricaria recutita was studied against carrageenan induced hind paw, arachidonic acid, acetic acid and complete Freund’s adjuvant (CFA)-induced arthritis in rats. The methanol extract of Matricaria recutita was administered at the dose of 100, 200 and 300 mg/kg body weight. In CFA-induced model ESR, vascular permeability, histamine release from blood and biochemical parameters were carried out. Chamomile methanol extract showed dose dependent significant  (P<0.001) anti-inflammatory activity by inhibition of rat paw oedema against carrageenan and arachidonic acid and inhibition of writhing induced by acetic acid. The significant anti-arthritic activity was observed with administration of Chamomile extract in the Freund’s adjuvant induced model of arthritis. Chronic treatment of extract of Chamomile showed significant decrease the development of arthritis and reduced ESR observed. It also reduced the histamine release from blood and vascular permeability at joint. The methanol extract of Matricaria recutita possess potential anti-inflammatory activity against acute and chronic inflammatory model
Isolating silkworm genomic DNA without liquid nitrogen suitable for marker studies
Genomic DNA was isolated from posterior silk gland of silkworms, Antheraea assama. Absolute alcohol was used as tissue fixing solution instead of grinding in liquid nitrogen, which yielded high molecular weight DNA (>40 kb). Samples yielded similar amount of DNA when fixed in absolute alcohol (400 μmg/g of silk gland tissue) and ground in liquid nitrogen (456 μmg/g of silk gland tissue). RAPD profile of the isolated DNA revealed high degree of polymorphism. The silkworms were analysed using 50 random primers among which 36 polymorphic primers gave 309 amplicons. The average amplicons per primer found to be 8.58 and 94.82% amplicons were polymorphic. Cluster analysis based on Jaccard’s similarity coefficients resulted in the formation of two main clusters with S9 on one cluster and the remaining strains on the other cluster. Jaccard’s similarity coefficients ranged from 0.122 to 0.863 indicating a high level of genetic diversity within muga silkworm collection. Isolated DNA was also suitable for cloning and restriction enzyme digestion. This method does not require liquid nitrogen for fixation, grinding or storage at -80°C, making it advantageous over other common protocols.Key words: Genomic, silkworms, muga, molecular breeding
Transcriptome analysis of stem wood of Nothapodytes nimmoniana (Graham) Mabb. identifies genes associated with biosynthesis of camptothecin, an anti-carcinogenic molecule
Camptothecin (CPT), a monoterpene indole alkaloid, is a potent inhibitor of DNA topoisomerase I and has applications in treating ovarian, small lung and refractory ovarian cancers. Stem wood tissue of Nothapodytes nimmoniana (Graham) Mabb. (family Icacinaceae) is one of the richest sources of CPT. Since there is no genomic or transcriptome data available for the species, the present work sequenced and analysed transcriptome of stem wood tissue on an Illumina platform. From a total of 77,55,978 reads, 9,187 transcripts were assembled with an average length of 255 bp. Functional annotation and categorization of these assembled transcripts unraveled the transcriptome
architecture and also a total of 13 genes associated with CPT biosynthetic pathway were identified in the stem wood
tissue. Four genes of the pathway were cloned to full length by RACE to validate the transcriptome data. Expression analysis of 13 genes associated with CPT biosynthetic pathway in 11 different tissues vis-Ă -vis CPT content analysis suggested an important role of NnPG10H, NnPSLS and NnPSTR genes in the biosynthesis of CPT. These results indicated that CPT might be synthesized in the leaves and then perhaps exported to stem wood tissue for storage
Open channel flow within and above a layered vegetation: Experiments and first-order closure modeling
Panton-Valentine Leukocidin Producing Staphylococcus Aureus Facial Pyomyositis Causing Partial Cavernous Sinus Thrombosis
We present a case of subtotal cavernous sinus thrombosis secondary
to Panton-Valentine leukocidin–associated Staphylococcus aureus
pyomyositis of the muscles of mastication in a previously healthy child,
who was successfully managed with no residual disease. He was found to
have a factor V Leiden heterozygous mutation. We highlight the propensity
of Panton-Valentine leukocidin Staphylococcus aureus to induce venous
thrombosis at any site but with potential for more severe consequences in
the head. We highlight pyomyositis as a differential for periorbital cellulitis
and discuss the significance of the factor V Leiden mutation
Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica)
This report describes the cloning and expression of a gene lycopene epsilon cyclase, (LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was identified from an SSH library constructed for quality trait in tea. 5’ and 3’ RACE (rapid-amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology studies showed that the deduced amino acid sequence of LCYE gene had the highest sequence identity of up to 84% with Vitis vinefera. The cloned gene was successfully expressed in a PET based Escherichia coli expression system. The size of the expressed protein was 59615 Daltons. A suppression subtractive library was constructed using a quality clone H3111 (tester) and a garden series clone T3E3 (driver).Key words: Carotenoid, RACE, heterologous expression, lutein, tea
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