Phytochemical analysis using GC-FID, FPLC fingerprinting, antioxidant, antimicrobial, anti- inflammatory activities analysis of traditionally used Eucalyptus globulus essential oil

Eucalyptus globules is an widely distributed in tropical and subtropical regions. It has been widely used as folk medicine, and folk cosmetic owing to its antioxidant values. Despite its importance, phytochemical and pharmacological studies remain infancy. This study was aimed at extraction of essential oil by steam-distillation and evaluation of bioactive components, antioxidant, antimicrobial, anti-inflammatory activities along with analysis by UV-VIS, FT-IR and Fluorescent techniques. Fast protein liquid chromatography (FPLC) was used to confirm the presence of polyphenols. Different antioxidant activities like DPPH., ABTS.+, .OH, superoxide, nitric oxide and reducing power of the essential oil. Essential oil was analyzed by UV-VIS, FT-IR and Fluorescent techniques. In vitro antimicrobial activity was also monitored. FT-IR fingerprint qualitative analysis was performed using commercial standards. Considerable amount of flavonoids were detected in essential oil.  Oil exhibited considerable scavenging activities of ABTS.+, .OH, superoxide, nitric oxide and reducing power. UV-VIS, FT-IR analysis revealed the presence of polyphenolics in essential oil. Fluorescent spectroscopy revealed the presence of fluorophores in essential oil. FPLC and FT-IR fingerprint analysis revealed the presence of bioactive constituents like rutin, tannic acid, vanillic acid and ascorbic acid in the essential oil. A strong anti-inflammatory activity of oil was observed using fluorescent spectroscopy. An appreciable in vitro antibacterial activity against gram-negative bacteria like Acetobacter aceti and Pseudomonas aeruginosa was detected. The data provides the scientific support to the use of essential oil from Eucalyptus globules as a potent herbal source of bioactive compounds possessing natural antioxidant activities in food and pharmaceutical industries

Phytochemical profile, in-vitro antioxidant, anti-diabetic and anti- inflammatory activities of traditionally used Euphorbia hirta (L.) growing under wild conditions of Northern Punjab

Euphorbia spp., are the widely distributed and traditionally used herbal plants due to their diverse biological activities. The aim of this study was comparative extraction of secondary metabolites with different solvents like acetone, ethanol, methanol and water and assessment of their biological activities from Euphorbia hirta L. Plant extracts from dried powder were prepared in different solvents. Phytochemicals like: phenolics, flavonoids and condensed tannins were estimated using standard assays. GC-FID studies were conducted to find out the presence of various bioactive compounds. Fingerprint analysis using UV, FT-IR and fluorescent spectroscopy was also conducted. Antioxidant activities using various assays, anti-diabetic and anti-inflammatory potential of extracts were evaluated. Fingerprint analysis using, UV-, FT-IR and fluorescent spectroscopy specified the occurrence of adequate level of polyphenolics in all solvent extracts. Amongst all solvents acetone gave higher yield of polyphenolics in-toto. GC-FID analysis of acetone extracts depicted the presence of bioactive compounds like major 1-naphthalenepropanol.  All extracts depicted enough level of antioxidant activities viz: DPPH, ABTS, hydroxyl radical, iron chelating and lipid peroxidation, but in a solvent dependent manner. Adequate anti-diabetic activity and anti-inflammatory activities were also observed in extracts. The results specified that by using suitable solvents bioactive compounds from Euphorbia species can be extracted and used as therapeutic agents having potential biological activities

Evaluation of the Acute Phase Reactants CRP in Unstable Angina Cases

Unstable angina, a syndrome of symptoms caused by ischemia of the heart muscles, is both frightening and disabling and may herald acute myocardial infarction. The immediate precipitating events of the atherosclerotic plaque responsible for that critical degree of ischemia resulting in the syndrome of unstable angina are progression of atherosclerosis platelet aggregation, thrombosis and vasospasm. Acute phase reactants are proteins in the plasma whose levels increase during acute inflammatory states or secondary to certain types of tissue damage. A cross-sectional descriptive study was done in Safdarjung Hospital on 40 subjects and controls to establish the acute phase reactants CRP in unstable angina cases and their relationships. In our study, we found that CRP levels are increased in cases as compared to controls, thus showing a direct correlation

Phytochemical profile, in-vitro antioxidant, anti-diabetic and anti- inflammatory activities of traditionally used Euphorbia hirta (L.) growing under wild conditions of Northern Punjab

Euphorbia spp., are the widely distributed and traditionally used herbal plants due to their diverse biological activities. The aim of this study was comparative extraction of secondary metabolites with different solvents like acetone, ethanol, methanol and water and assessment of their biological activities from Euphorbia hirta L. Plant extracts from dried powder were prepared in different solvents. Phytochemicals like: phenolics, flavonoids and condensed tannins were estimated using standard assays. GC-FID studies were conducted to find out the presence of various bioactive compounds. Fingerprint analysis using UV, FT-IR and fluorescent spectroscopy was also conducted. Antioxidant activities using various assays, anti-diabetic and anti-inflammatory potential of extracts were evaluated. Fingerprint analysis using, UV-, FT-IR and fluorescent spectroscopy specified the occurrence of adequate level of polyphenolics in all solvent extracts. Amongst all solvents acetone gave higher yield of polyphenolics in-toto. GC-FID analysis of acetone extracts depicted the presence of bioactive compounds like major 1-naphthalenepropanol.  All extracts depicted enough level of antioxidant activities viz: DPPH, ABTS, hydroxyl radical, iron chelating and lipid peroxidation, but in a solvent dependent manner. Adequate anti-diabetic activity and anti-inflammatory activities were also observed in extracts. The results specified that by using suitable solvents bioactive compounds from Euphorbia species can be extracted and used as therapeutic agents having potential biological activities