First measurements of the flux integral with the NIST-4 watt balance

In early 2014, construction of a new watt balance, named NIST-4, has started at the National Institute of Standards and Technology (NIST). In a watt balance, the gravitational force of an unknown mass is compensated by an electromagnetic force produced by a coil in a magnet system. The electromagnetic force depends on the current in the coil and the magnetic flux integral. Most watt balances feature an additional calibration mode, referred to as velocity mode, which allows one to measure the magnetic flux integral to high precision. In this article we describe first measurements of the flux integral in the new watt balance. We introduce measurement and data analysis techniques to assess the quality of the measurements and the adverse effects of vibrations on the instrument.Comment: 7 pages, 8 figures, accepted for publication in IEEE Trans. Instrum. Meas. This Journal can be found online at http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=1

Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis

An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth

Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1)

RGTA is a novel matrix therapy for ischemic stroke

CERVOXYInternational audienc

A novel ischemic stroke matrix therapy based on the RGTA

CERVOXY COLLNational audienc

Comité de rédaction

Comité de rédaction. In: Revue de géographie de Lyon, vol. 53, n°1, 1978. p. 2

An analogue of heparan sulfate as a novel neuroprotective and neuroregenerative agent for ischemic stroke

CERVOXYInternational audienc