Additional file 1: Table S1. of The complete genome sequence of the rumen bacterium Butyrivibrio hungatei MB2003

Associated MIGS record for B. hungatei MB2003, which links to the SIGS supplementary content website. (DOCX 17 kb

Additional file 1: Table S1. of The complete genome sequence of the rumen methanogen Methanobrevibacter millerae SM9

Associated MIGS record for M. millerae SM9, which links to the SIGS supplementary content website. (DOC 70 kb

Additional file 1: Table S1. of The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1

Associated MIGS record for CM1, which links to the SIGS supplementary content website

Additional file 1: Table S1. of The complete genome sequence of Eubacterium limosum SA11, a metabolically versatile rumen acetogen

Associated MIGS record for M. millerae SM9, which links to the SIGS supplementary content website. (DOC 70 kb

Additional file 1: Table S1. of The complete genome sequence of the methanogenic archaeon ISO4-H5 provides insights into the methylotrophic lifestyle of a ruminal representative of the Methanomassiliicoccales

Associated MIGS record. (DOC 72 kb

<i>Bifidobacterium longum</i> general genome features.

<p><i>Bifidobacterium longum</i> general genome features.</p

¹H and ¹³C NMR chemical shifts (δ, ppm) of the exopolysaccharide (recorded at 338 K) and the tetrasaccharide os211 (recorded at 300 K) from <i>B</i>. <i>longum</i> 35624.

<p>¹H and ¹³C NMR chemical shifts (δ, ppm) of the exopolysaccharide (recorded at 338 K) and the tetrasaccharide os211 (recorded at 300 K) from <i>B</i>. <i>longum</i> 35624.</p

B. longum 35624 EPS proton and carbon signals.

<p>(<b>A</b>) A selected region of the multiplicity-edited, gradient enhanced ¹H, ¹³C-HSQC NMR spectrum of the exopolysaccharide. Letters denote the residues as given in the structural formula and arabic numerals denote the respective pyranose position. Resonances from anomeric carbons/protons, glycosylation sites and resolved signals are annotated. (<b>B</b>) Selected region of the ¹H, ¹³C-HSQC-TOCSY NMR spectrum (600 MHz) of the acid-treated <b>35624</b> EPS. Arabic numerals before and after oblique stroke denote carbons and protons, respectively.</p

Mild acid hydrolysis of EPS.

<p>(A) Separation of EPS fragments by PGC HPLC with MS/MS detection. The extracted ion chromatogram for mass 1008.39 Da shows four peaks. Their reducing end sugar was clearly revealed by ESI-MS/MS. Their assignment as either Gal or Glc and the interpretation in terms of fragment structures was done <i>a posteriori</i> based on MALDI-TOF data and on knowledge of the EPS structure. (B) Example of a MALDI-TOF/TOF fragment spectrum showing b-ions from the non-reducing and y- and y´ (= ^1,5x) -ions from the reducing end.</p

Phylogenetic tree based on the B. longum core-genome.

<p>(A) The <i>B</i>. <i>longum</i> subsp. <i>longum</i> phylogenetic group. (B) The <i>B</i>. <i>longum</i> subsp. <i>infantis</i> phylogenetic group. <i>B</i>. <i>longum</i> subsp. <i>longum</i> and <i>B</i>. <i>longum</i> subsp. <i>infantis</i> type strains are indicated in blue text. <i>Lactobacillus salivarius</i> was included as an outlier.</p