Mutation increasing β-carotene concentrations does not adversely affect concentrations of essential mineral elements in pepper fruit

<div><p>Vitamin and mineral deficiencies are prevalent in human populations throughout the world. Vitamin A deficiency affects hundreds of millions of pre-school age children in low income countries. Fruits of pepper (<i>Capsicum annuum</i> L.) can be a major dietary source of precursors to Vitamin A biosynthesis, such as β-carotene. Recently, pepper breeding programs have introduced the orange-fruited (<i>of</i>) trait of the mutant variety Oranzheva kapiya, which is associated with high fruit β-carotene concentrations, to the mutant variety Albena. In this manuscript, concentrations of β-carotene and mineral elements (magnesium, phosphorus, sulphur, potassium, zinc, calcium, manganese, iron and copper) were compared in fruit from P31, a red-fruited genotype derived from the variety Albena, and M38, a genotype developed by transferring the orange-fruited mutation (<i>of</i>) into Albena. It was observed that fruit from M38 plants had greater β-carotene concentration at both commercial and botanical maturity (4.9 and 52.7 mg / kg fresh weight, respectively) than fruit from P31 plants (2.3 and 30.1 mg / kg fresh weight, respectively). The mutation producing high β-carotene concentrations in pepper fruits had no detrimental effect on the concentrations of mineral elements required for human nutrition.</p></div

Assessment of Ionic Interferences to Nitrate and Potassium Analyses with Ion-Selective Electrodes

Ion-selective electrodes (ISEs) are simple tools used for rapid measurement of nitrate nitrogen (NO3-N) and potassium (K) concentrations in plant sap. With the development of best management practices (BMPs), interest exists in using ISEs for soil leachate and soil and fertilizer solutions. Nitrate N and K concentrations in the 0 to 10,000 mg L–1 ISE working range were measured in diluted solutions of common salts to assess ionic interference of calcium (Ca2+), ammonium (NH4+), chloride (Cl–), and sulfate (SO42–). The effects of meter (replication) were unexpectedly significant in one out of three ranges for NO3-N and K (P values of 0.50, 0.72, and 0.01 for NO3-N and 0.99, 0.01, and 0.74 for K, for the 0–100, 100–1,000 and 1,000–10,000 mg L–1 ranges, respectively). The responses of calculated NO3-N and K concentrations to measured NO3-N and K concentrations were linear, but slopes ranged from 0.85 to 1.54, from 0.24 to 2.72, and from 0.93 to 5.48 for NO3-N and from 0.80 to 1.01, from 0.71 to 1.39, and from 0.93 to 2.21 for K for the 0–100, 100–1,000, and 1,000–10,000 mg L–1 measuring ranges, respectively. All slopes were significantly different from zero, and several were significantly different from each other and the 1:1 line. Pairwise slope comparisons conducted with covariance analysis showed that SO42– alone interfered with NO3-N measurements at concentrations ranging from 34 to 171 mg L–1, which was less than the manufacturer’s information, and by its presence in combination with K+, NH4+, Ca2+, and Cl– within the medium and high concentration ranges. Potassium measurements were not subject to interference from the ions tested for all three concentration ranges. These results highlight the importance of using quality assurance / quality control (QA/QC) samples in the set of unknown samples to detect inacceptable departure from linearity in routine analysis. The increase in measurement variability from one range to the next showed the importance of keeping measurements within a single concentration range by using dilutions. Hence, ISEs may be used for field measurements of NO3-N and K concentrations in soil leachate as well as soil and nutrient solutions and are therefore a practical BMP tool. However, ISEs should not be used as substitutes for the laboratory methods when official measurements are needed

Color biogenesis data of tomatoes treated with hot-water and high temperature ethylene treatments

Controlled postharvest stresses were used to induce the synthesis of carotenoids in tomato fruit. The accumulation of carotenoids was observed by the change of color of the tomato fruit from green to red. This change of color was monitored by the a* value and hue of the CIELAB* color coordinates in which the a* value increased following a sigmoidal curve and hue decreased in a similar trend. This sigmoidal curve marked the transition from chloroplasts to chromoplasts; in other words, the change of color tracked the disorganization or degreening, which was simultaneously accompanied by chromoplast biogenesis or red color development when tomatoes were at the Turning stage of development. The color data and photographic images provides information on how heat stress affected the synchronicity of chloroplast disorganization and chromoplast biogenesis in the early developmental stages of tomato ripening