Analysis of the relationship between the KRAS G12V oncogene and the Hippo effector YAP1 in embryonal rhabdomyosarcoma

The authors acknowledge funding from Sarcoma UK, grant number: SUK09.2015.Peer reviewedPublisher PD

Stability of multidimensional black holes: complete numerical analysis

We analyze evolution of gravitational perturbations of D-dimensional Schwarzschild, Reissner-Nordstr\"om, and Reissner-Nordstrom-de Sitter black holes. It is known that the effective potential for the scalar type of gravitational perturbations has negative gap near the event horizon. This gap, for some values of the parameters Q (charge), Lambda (cosmological constant) and D (number of space-time dimensions), cannot be removed by S-deformations. Thereby, there is no proof of (in)stability for those cases. In the present paper, by an extensive search of quasinormal modes, both in time and frequency domains, we shall show that spherically symmetric static black holes with arbitrary charge and positive (de Sitter) lambda-term are stable for D=5, 6, >...11. In addition, we give a complete numerical data for all three types (scalar, vector and tensor) of gravitational perturbations for multi-dimensional black holes with charge and Lambda-term. The influence of charge, Lambda-term and number of extra dimensions on black hole quasinormal spectrum is discussed.Comment: 12 pages, RevTe

Quasinormal modes of black holes immersed in a strong magnetic field

We found quasinormal modes, both in time and frequency domains, of the Ernst black holes, that is neutral black holes immersed in an external magnetic field. The Ernst solution reduces to the Schwarzschild solution, when the magnetic field vanishes. It is found that the quasinormal spectrum for massless scalar field in the vicinity of the magnetized black holes acquires an effective "mass" $\mu = 2 B m$, where m is the azimuthal number and B is parameter describing the magnetic field. We shall show that in the presence of a magnetic field quasinormal modes are longer lived and have larger oscillation frequencies. The perturbations of higher dimensional magnetized black holes by Ortaggio and of magnetized dilaton black holes by Radu are considered.Comment: 5 pages, RevTe

Quasi-normal modes of the scalar hairy black hole

We calculate QNMs of the scalar hairy black hole in the AdS background using Horowitz-Hubeny method for the potential that is not known in analytical form. For some black hole parameters we found pure imaginary frequencies. Increasing of the scalar field mass does not cause the imaginary part to vanish, it reaches some minimum and then increases, thus in the case under consideration the infinitely long living modes (quasi-resonances) do not appear.Comment: 17 pages, 17 figures, LaTe

CONSTRUÇÃO E VALIDAÇÃO DE INSTRUMENTO PARA AVALIAÇÃO DAS MEDIDAS DE BIOSSEGURANÇA EM BIOTERRORISMO PELOS BOMBEIROS

Objetivo: Este estudo teve como objetivo desenvolver e validar um instrumento de avaliação das medidas de Biossegurança adotadas pelos bombeiros militares, para a contenção dos agentes biológicos frente a um evento de bioterrorismo. Métodos: Tratou-se de um estudo descritivo de abordagem quantitativa, de desenvolvimento metodológico e do tipo de validação de conteúdo de um instrumento de avaliação. O estudo foi dividido nas fases de desenvolvimento e validação do instrumento. Para o desenvolvimento do instrumento foram feitas revisões da literatura e para a validação do instrumento foi utilizado o método Delphi. Para o estudo foram incluídos 6 juízes que avaliaram o instrumento através da escala numérica tipo Likert. Resultados: Os juízes avaliaram que o instrumento proposto está bem estruturado, possuindo boa clareza e coesão de escrita, com aplicabilidade no campo de estudo e de grande relevância, principalmente em vista a ausência desse tipo de instrumento para a população de bombeiros militares. Para avaliar a concordância entre os juízes foi utilizado o índice de Validade de Conteúdo, que alcançou 98% de concordância e o Índice de Fidedignidade Interavaliadores, que alcançou os conceitos bom e muito bom, mostrando baixa variância das respostas dos juízes, sendo estatisticamente válido. Conclusão: O estudo descreveu o processo de construção e validação do instrumento, provando ser apropriado e confiável para ser utilizado

Methodology for a Comprehensive Health Impact Assessment in Water Supply and Sanitation Programmes for Brazil

Based on the broader concept of health proposed by the Pan-American Health Organization/World Health Organization (PAHO/ WHO), 2018, and the absence in the literature of indices that translate the causal relationship between sanitation and health, a methodology for assessing the health impact of a water and sanitation programmes, known as a Health Impact Assessment (HIA), was developed, specifically in the Brazilian context, and focused on a school in the northeast of the country. Through exploratory and descriptive evidence, and using documentary research as a method, a retrospective survey was carried out from 2000 to 2022 using documents proposing evaluation methodologies. A single document was found to fit the research objective, which was used to develop the proposed HIA methodology. Development of the methodology consisted of two stages: definition of the health dimensions and selection of the indicators making up each dimension. The HIA methodology was then applied to a school in northeast Brazil to test its use, before a water-efficient management intervention was going to be used. The overall score of 46% indicated that there was room for improvement, which the new management approach could facilitate. This methodology is therefore proposed to be an instrument for the evaluation of public water and sanitation policies, thus assisting managers in the decision-making process and in guiding sanitation programs and plans

Mixed Endometrial Epithelial Carcinoma: Epidemiology, Treatment and Survival Rates-A 10-Year Retrospective Cohort Study from a Single Institution

Mixed endometrial carcinoma (MEEC) refers to rare endometrial tumours that are composed of two or more distinct histotypes, at least one of which is serous or clear cell. The aim of this study was to evaluate the epidemiology, treatment outcomes and survival rates of patients with mixed endometrial carcinoma. The medical records of 34 patients diagnosed with MEEC between March 2010 and January 2020 were reviewed retrospectively. Clinicopathological variables and treatment strategies were assessed, and overall survival and disease-free survival rates were evaluated. The histology of endometrioid and serous component was found in 26 (76.5%) patients, followed by serous and clear-cell components (5/34, 14.5%) and mixed endometrioid serous and clear-cell components (3/34, 8.8%). The median age at diagnosis was 70 years (range 52-84), and the median follow-up time was 55 months. The 5-year disease-free survival and the 5-year overall survival were 50.4% and 52.4%, respectively. Advanced disease stage was identified as an independent predictor of inferior disease-free (<0.003) and overall survival (p < 0.001). Except for stage, none of the traditional prognostic factors was associated with disease recurrence or death from disease. MEECs represent rare high-risk endometrial carcinomas with significant diagnostic and treatment challenges. Undoubtedly, the implementation of a molecular analysis can offer further diagnostic and management insights

The Prognostic Characteristics and Recurrence Patterns of High Grade Endometrioid Endometrial Cancer: A Large Retrospective Analysis of a Tertiary Center

High grade endometrioid endometrial cancer (HGEEC) is a heterogeneous group of tumors with unclear prognostic features. The aim of the present study is to evaluate the independent risk factors for recurrence and mortality and to describe the recurrence patterns of HGEEC. Ninety-six consecutive cases of HGEEC treated with primary surgery in a single Tertiary Center were retrospectively reviewed. Clinicopathological and treatment details were recorded, and all patients were closely followed up. Disease-free, overall and cancer-specific survival rates were 83.8%, 77.8% and 83.6%, respectively. Cervical stromal involvement was independently related to recurrence (HR = 25.67; 95%CI 2.95-223.30; p = 0.003) and cancer-related death (HR = 15.39; 95%CI 1.29-183.43; p = 0.031) after adjusting for other pathological and treatment variables. Recurrence rate was 16%, with 60% of these cases having lung metastases and only one case with single vaginal vault recurrence. 81.81% of the recurrences presented with symptoms and not a single recurrence was diagnosed in routine follow-up clinical examination. In conclusion, the recurrence pattern may suggest that patient-initiated follow-up (PIFU) could be considered a potential alternative to clinical-based follow-up for HGEEC survivors, especially for patients without cervical involvement and after two years from treatment. Additional caution is needed in patients with cervical stromal involvement