Customer lifetime values

Diplomová práca sa zaoberá problematikou celoživotnej hodnoty zákazníka v stavebnej spoločnosti a všeobecne vo firmách. Teoretická časť je zameraná na význam hodnoty zákazníka pre spoločnosť a na odlišné typy výpočtov celoživotnej hodnoty. V praktickej časti sú spracované konkrétne výpočty celoživotnej hodnoty zákazníkov v stavebnej spoločnosti, ich analýza a vyhodnotenie.Diploma thesis is focusing on issues of a customer lifetime value in the construction company and in the companies in general. Theoretical part is focusing on the importance of customer value in the company and on various types of calculations of customer lifetime value. Practical part is describing specific calculations of customer lifetime value in the construction company, their analysis and evaluations.

Provenance of Albian to Cenomanian exotics-bearing turbidites in the Western Carpathians : a heavy mineral analysis

Throughout the Cretaceous, Tethyan oceanic branches gradually closed, and various ophiolites became obducted and eroded. Their remnants, however, provide an abundance of exotic clasts of unknown origin. Sandstone samples from the oldest, Albian exotics-bearing strata of the Pieniny Klippen Belt and Central Western Carpathians were analysed for heavy minerals. These samples were dominated by a high content of chrome-spinels, zircon, tourmaline, apatite and rutile. Titanite, kyanite, monazite, epidote, sillimanite and staurolite were much less abundant. Garnet was generally also rare; however, it was locally common, as were blue amphiboles, pyroxenes and kyanite. The spinels found in the samples were predominantly derived from harzburgites (supra-subduction peridotites and volcanic rocks). The blue amphiboles represented glaucophanes to ferroglaucophanes, and were derived from HP/LT metabasites. Pyroxenes (enstatite, less commonly augite and diopside) most likely came from coeval volcanics. Most of the tourmalines were derived from metasedimentary rocks and locally from granitoids. Furthermore, some have a complex zonation with two phases of tourmaline, or tourmaline intergrown with quartz. These were likely derived from ophiolitic sources. The results from our analysis indicate a dominance of ophiolites and older sediments with local input of continental crust metamorphic rocks. A resulting palaeogeographic reconstruction involves secondary doubling of the Neotethys suture zone and its lateral shift north of the Central Western Carpathians, which formed a common source for exotics in the Pieniny Klippen Belt and the Central Western Carpathians

Synthesis of C3-Substituted N1-tert-Butyl 1,2,4-Triazinium Salts via the Liebeskind–Srogl Reaction for Fluorogenic Labeling of Live Cells

We recently described the development and application of new bioorthogonal conjugation, the triazinium ligation. To explore the wider application of this reaction, in this work we introduce a general method for synthesizing C3-substituted triazinium salts based on the Liebeskind–Srogl cross-coupling reaction and catalytic thioether reduction. These methods enabled the synthesis of triazinium derivatives for investigating the effect of different substituents on the ligation kinetics and stability of the compounds under biologically relevant conditions. Finally, we demonstrate that the combination of coumarin fluorophore attached to position C3 with a C5-(4-methoxyphenyl) substituent, yields a fluorogenic triazinium probes suitable for no-wash, live-cell labeling. The developed methodology represents a promising synthetic approach to the late-stage modification of triazinium salts, potentially widening their application in bioorthogonal reactions

Triazinium Ligation: Bioorthogonal Reaction of N1-alkyl 1,2,4-Triazinium Salts

The development of reagents that can selectively react in complex biological media is an important challenge. Here we show that N1-alkylation of 1,2,4-triazines yields the corresponding triazinium salts, which are three orders of magnitude more reactive in reactions with strained alkynes than the parent 1,2,4-triazines. This powerful bioorthogonal ligation enables efficient modification of peptides and proteins. The positively charged N1-alkyl triazinium salts exhibit favorable cell permeability, which makes them superior for intracellular fluorescent labeling applications when compared to analogous 1,2,4,5-tetrazines. Due to their high reactivity, stability, accessibility and improved water solubility, the new ionic heterodienes represent a valuable addition to the repertoire of existing modern bioorthogonal reagents