28 research outputs found

    Kajian Pengelolaan Lahan Subdas Secang Kulonprogo YOGYAKARTA

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    Penelitian ini bertujuan untuk mengevaluasi kemampuan lahan, menyusun arahan penggunaanlahan dan mengkaji pengelolaan lahan SubDAS Secang. Metode yang digunakan dalam penelitianadalah sampel terpilih pada 48 satuan lahan. Penelitian menunjukkan bahwa kemampuan lahanSubDAS Secang terdiri atas kelas lahan I seluas 187 ha, kelas lahan II seluas 147 ha, kelas lahan IIIseluas 515,2 ha, kelas lahan IV seluas 1522,7 ha, kelas lahan V seluas 7,3 ha dan kelas lahan VI seluas1223,2 ha. Arahan penggunaan lahan SubDAS Secang berupa pertanian lahan basah seluas 326,85 ha,kawasan permukiman dan budidaya tanaman semusim seluas 200,55 ha, kawasan budidaya tanamanlahan kering seluas 525,81 ha, kawasan budidaya tanaman tahunan seluas 1981,31 ha, kawasanpenyangga seluas 716,54 ha. Pengelolaan lahan memberikan pedoman pemanfaatan lahan; daerah hilirsebagai daerah pemanfaatan untuk pertanian irigasi; daerah tengah diperuntukan permukiman danpembudidayaan tanaman lahan kering; serta daerah hulu sebagai daerah imbuhan diperuntukkanwanatani dan hutan penyangga

    Analisis Pengaruh Budaya Organisasi Dan Kompensasi Terhadap Kinerja Karyawan Dengan Motivasi Sebagai Variabel Intervening (Studi Kasus Pada PT. Lg Bagian Penjualan Indonesia Semarang)

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    The problems that occurred in the employee portion of sales LG Indonesia Semarang is adecline in performance is indicated by not achieving the target for 2015. The employeeperformance and motivation is also thought to be influenced by factors of organizationalculture and also compensation deemed not feasible by most employees. This study aimedto analyze the influence of organizational culture on the motivation and compensationand employee performance parts sales LG Indonesia Semarang. The population used inthis study were all employees of LG Indonesia Semarang. The sampling technique usedwas purposive sampling. Criteria samples taken were all employees of the salesdepartment LG Indonesia Semarang who have worked more than two years are 71nurses. The method of collecting the data in this study using questionnaires andinterviews. Methods of data analysis using path analysis. Based on the research,organizational culture and compensation have a positive effect on motivation andperformance, while motivation is also a positive effect on performance. Based on theresults Sobel Test to determine whether there is mediating the relationship between theindependent and dependent variables, it is known that motivation mediates the effect ofcompensation and organizational culture on performance

    Additional file 1: of Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

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    An Excel spreadsheet with three tabs listing 1) datasets generated in this study, 2) datasets used from the publically domain, together with key parameters, and 3) number of datasets used per analysis. (XLSX 21 kb

    Effect of brain region on kinetic parameters.

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    <p>Population histograms of the range of kinetic parameters observed in cerebellar astrocytes (top row) and cortical astrocytes (bottom row) stimulated with 1 mM glutamate. Rise time and latency are measured in seconds (s), peak amplitude as maximal fold change in fluorescence intensity over baseline (F<sub>max</sub>/F<sub>0</sub>), and area under curve as the integral of F/F<sub>0</sub> over time (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026889#s4" target="_blank">Methods</a> for further details). Difference was measured for all agonists at 1 mM. Results are summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026889#pone-0026889-t005" target="_blank">Table 5</a>.</p

    Pharmacological characterization of receptor types.

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    <p>Representative traces of changes in fluorescence intensity over time after addition of pharmacological agonists (at 100 µM) selective for P2X receptors (BzATP), P2Y receptors (2-MeS-ADP), AMPA/kainate receptors (KA), mGlu receptors (DHPG), H1 receptors (Pyri Di) and H2 receptors (Dim Di), in cerebellar (<b>A</b>) and cortical (<b>B</b>) cells. <b>C</b>) Percentage of cerebellar and <b>D</b>) cortical cells responding to each agonist. Data for each agonist are from 3–6 coverslips of cells, obtained from 2–3 culture preparations. Total cells tested for each agonist ranged from 64 to 202.</p

    Differences in kinetic parameters for spontaneous Ca<sup>2+</sup> signals in unstimulated cells.

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    <p>Three control populations (cells exposed to buffer exchange in the absence of agonist) were compared. Values of difference (<i>D</i>) were calculated as outlined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026889#s4" target="_blank">Methods</a>. Lower values indicate a greater degree of similarity between the distributions.</p

    Ca<sup>2+</sup> signals evoked by receptor agonists in cultured astrocytes.

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    <p><b>A</b>) Left panel: Confocal image of cerebellar astrocytes stained for the astrocyte specific glial fibrillary acidic protein (GFAP, green) and a nuclear stain (DAPI, blue) captured using a point scanning confocal microscope. Scale bar = 20 µM. Right panel: Representative normalized fluorescence changes in single live cells loaded with fluo-4 AM and stimulated with 100 µM ATP, glutamate or histamine (as indicated). <b>B</b>) Confocal image and representative fluorescence changes in cortical astrocytes under the same conditions as in (A). <b>C</b>) Percentage of cells responding to each agonist (where response is defined as an increase in fluorescence of greater than 1.045 fold), for both cerebellar (left panel, filled bars) and cortical (right panel, unfilled bars) cultures. Data for each agonist are from 3–8 coverslips of cells, obtained from 2–3 culture preparations. Total cerebellar cells tested: 175 (100 µM ATP); 580 (100 µM glutamate), 161 (100 µM histamine). Total cortical cells tested: 129 (100 µM ATP); 504 (100 µM glutamate), 134 (100 µM histamine). Statistical significance of differences in the percentage of responding cells was tested by Fisher's exact test. *p <0.05 or ** p <0.001 compared to ATP, and †p <0.05 or ††p <0.001 compared to glutamate.</p

    Measurement of difference between populations.

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    <p><b>A</b>) Two populations of idealized data (Gaussian distribution, mean = 5, SD = 1, area = 1, n = 5000) were offset by multiples of the standard deviation. <b>B</b>) Subtraction of the (normalized) population <i>B</i> from population <i>A</i> shows increasing difference. Quantification of difference (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026889#s4" target="_blank">Methods</a>) gives a value of <i>D</i> that rises as overlap between populations decreases. <b>C)</b> Histograms of kinetic parameters from typical control wells that received no stimulus (spontaneous Ca<sup>2+</sup> signalling in cerebellar astrocytes receiving buffer exchange without agonist). A comparison was made of two control populations (Con A and Con B) that served as zero agonist controls in different imaging experiments. Note broad range of latencies, suggesting no correlation of spontaneous Ca<sup>2+</sup> signals with mixing time. Lower panels show subtraction histograms and calculated <i>D</i> values for these spontaneous signals.</p
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