The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3)

The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA) as active compound. Silver nitrate was taken as the metal precursor (AgNPs) and sodium alginate (Alg) as a reducing agent. The EA-Alg-AgNPs nanocomposite was characterized using transmission electron microscopy (TEM), zeta potential, and in vitro release kinetics. The particles thus obtained were spherical in shape and having an average particles size of 10 nm, zeta potentials of –8.2 mV, and the release kinetics of EA from nanocomposite was following Hixson-Crowell kinetics models with R 2 = 0.9956. The cytotoxicity potential of free EA, Alg-AgNPs and the EA-Alg-AgNPs nanocomposite may be determined using a normal mouse fibroblast cells (3T3) and breast cancer cells (MCF-7). EA-Alg-AgNPs nanocomposite demonstrated a increased cytotoxicity effect when compared to free EA on MCF-7 cells with 15.3% cell viability at 128 μg/mL; compared to 33.5% cell viability in a direct EA exposure. It is worth mentioning the cytotoxicity of Alg-AgNPs against MCF-7 shows 28% viability at 128 μg/mL

Sensitive and selective spectrophotometric determination of hg(ii), ni(ii), cu(ii) and co(ii) using iminodibenzyl and 3-chloroiminodibenzyl as new reagents and their applications to industrial effluents and soil samples

Iminodibenzyl (IDB) and 3-chloroiminodibenzyl (Cl-IDB) are proposed as a new class of spectrophotometric reagents for the determination of micro amounts of mercury(II), nickel(II), copper(II) and cobalt(II) in the presence of 3-methyl-2-benzothiazoline hydrazone hydrochloride hydrate (MBTH) or 4-aminoantipyrine (AAP) as electrophilic coupling reagents. The reaction is carried out in neutral aqueous medium. The blue colour formed shows maximum absorbance at 660 nm using MBTH and 630 nm with AAP. The methods obey Beer's law. The molar absorptivity and Sandell's sensitivity show different values with different metals and reagents. The blue colour can also be extracted quantitatively which can be exploited as an efficient extractive spectrophotometric technique for the separation of metal ion from other interfering species. As many as 15 cations and 12 anions and/or complexing agents listed do not interfere. Both the methods have good reproducibility and can be satisfactorily applied in the determination of metals in industrial effluents and soil samples