Osteoinductive effects of preoperative dexamethasone in human dental pulp stem cells primary culture

Aim: The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques. Our objective was to evaluate the osteoinductive effect of DEX administered as a preoperative medication in primary cell culture of human dental pulp stem cell. Methodology: Cells from the third molar pulp were divided into two experimental groups, each with two preoperative medication protocols used in dental practice and differentiated by the intake of DEX in one of them. The assessment of proliferation, differentiation and viability through trypan blue, methylthiazol tetrazolium, and von Kossa and alizarin red assays, respectively, were held within fixed intervals: 7, 14, 21 and 28 days. Conclusion: This study has shown that DEX may influence in vitro human dental pulp stem cell behavior. Lay abstract: Dexamethasone (DEX) is often used as a preoperative drug in dental surgeries because it reduces pain and has favorable effects on other symptoms caused by the surgery. Additionally, when used in cell culture, its osteoinductor effect is observed. In vitro cell characterization is critical to ensure the development of therapeutic strategies used in tissue engineering. In this sense, this study used two preoperative medication protocols regularly used in dental practice. In Protocol A, patients did not intake DEXin Protocol B, patients took in tablets of DEX. It was possible to assess in vitro behavior of human dental pulp stem cells by applying those protocols.UNIFESP Univ Fed São Paulo, Escola Paulista Med, CTCMol, Ctr Cellular & Mol Therapy, São Paulo, BrazilUNIFESP Univ Fed São Paulo, Escola Paulista Med, Translat Surg, Dept Surg, São Paulo, BrazilBIOFABRIS, Natl Inst Sci & Technol, Biofabricat Inst, São Paulo, BrazilHosp Ipiranga, Oral & Maxillofacial Surg Dept, São Paulo, BrazilUNIFESP Univ Fed São Paulo, Escola Paulista Med, CTCMol, Ctr Cellular & Mol Therapy, São Paulo, BrazilUNIFESP Univ Fed São Paulo, Escola Paulista Med, Translat Surg, Dept Surg, São Paulo, BrazilWeb of Scienc

Patients with neurological sequelae and congenital deformations: postural stabilizer in dental treatment sessions

O objetivo deste trabalho foi verificar os beneficies que o estabilizador postural proporciona aos pacientes com sequela neurologica e malformacao congenita com massa corporea ate 30 Kg/m e altura superior a 110 cm. e aos academicos e profissionais em Odontologia. Foram realizaram 80 atendimentos odontologicos em dois momentos diferentes e de modo aleatorio, com e sem o uso do estabilizador postural em 40 pacientes cujas idades variaram entre 13 e 52 anos, sendo 21 do sexo masculino e 19 do feminino. Durante os atendimentos foram executadas as seguintes especialidades: Dentistica, Endodontia, Periodontia e Cirurgia. Os resultados obtidos mostraram que o uso do estabilizador postural propiciou diminuicao da ansiedade e aumento da seguranca nos pacientes, academicos e profissionais, outrossim verificamos melhora da postura ergonomica nos academicos e profissionais em Odontologia e do padrao de contracao muscular nos pacientes. O tempo de atendimento dos academicos nao diferiu do tempo dos profissionais apontando acentuada reducao do mesmoBV UNIFESP: Teses e dissertaçõe

Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development

Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.FAPESP[07-58856-7]FAPESP[07-51227-4]FAPESP[07-59488-1]NIH/NIDCR[R01DE016132]NIH/NIDCR[R03TW007665

Cytogenetic instability of dental pulp stem cell lines

Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. The heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.INCTBiofabrication InstituteINCT-Biofabrication InstituteCNPqCNPq [573661/2008-1]FAPESP [08/57860-3, 07-58856-7, 07-51227-4, 07-59488-1]FAPESPRede Biofab, IberoAmerican Network of BiofabricationBIOFABCYTEDRede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED [208RT0340]NIH/NIDCR [R01DE016132, R03TW007665]NIH/NIDC