Imaginarios colectivos de la violencia de los habitantes del Municipio de Pasto

Este trabajo de investigación, es una aproximación al análisis de la violencia colombiana en sus múltiples manifestaciones, expresadas por rostros humanos, cargados de terror, que no quieren seguir siendo las víctimas incondicionales de este terrible mal que se ha apoderado del país. Es una descripción y significación de los imaginarios de la violencia, que toma como pretexto a los habitantes del municipio de Pasto, para ser vista por los niños y adolescentes, mujeres y habitantes de la calle, ejecutivos y religiosos, guerrilleros, excombatientes y militares, quienes desde su perspectiva opinaron acerca de este fenómeno. En síntesis, los capítulos que aquí se presentan son, en primer lugar, la Justificación, que radica principalmente en la posibilidad de aportar una visión de la realidad del colectivo social, centrándose básicamente en el impacto de esta investigación. En segundo lugar, se hace una descripción de los Antecedentes del proyecto, en donde se citan brevemente algunos estudios de violencia que aportan en la construcción social desde lo local

TEMPORAL AND SPATIAL DISTRIBUTION OF POACEAE POLLEN IN AREAS OF SOUTHERN UNITED KINGDOM, SPAIN AND PORTUGAL

Overall, longer Poaceae pollen seasons coincided with earlier pollen season start dates. Winter rainfall noticeably affects the intensity of Poaceae pollen seasons in Mediterranean areas, but this was not as important in Worcester. Weekly data from Worcester followed a similar pattern to that of Badajoz and Évora but at a distance of more than 1500 km and 4-5 weeks later

Modulated model predictive control with optimized overmodulation

Finite Set Model Predictive Control (FS-MPC) has many advantages, such as a fast dynamic response and an intuitive implementation. For these reasons, it has been thoroughly researched during the last decade. However, the wave form produced by FS-MPC has a switching component whose spread spectrum remains a major disadvantage of the strategy. This paper discusses a modulated model predictive control that guarantees a spectrum switching frequency in the linear modulation range and extends its optimized response to the overmodulation region. Due to the equivalent high gain of the predictive control, and to the limit on the voltage actuation of the power converter, it is expected that the actuation voltage will enter the overmodulation region during large reference changes or in response to load impacts. An optimized overmodulation strategy that converges towards FS-MPC’s response for large tracking errors is proposed for this situation. This technique seamlessly combines PWM’s good steadystate switching performance with FS-MPC’s high dynamic response during large transients. The constant switching frequency is achieved by incorporating modulation of the predicted current vectors in the model predictive control of the currents in a similar fashion as conventional Space-Vector Pulse Width Modulation (SV-PWM) is used to synthesize an arbitrary voltage reference. Experimental results showing the proposed strategy’s good steady-state switching performance, its FS-MPC-like transient response and the seamless transition between modes of operation are presented for a permanent magnet synchronous machine drive

Influências de usinas hidrelétricas no funcionamento hidro-ecológico do Pantanal Mato-Grossense - recomendações.

Esta publicação tem como objetivo registrar e divulgar as recomendações resultantes do Workshop ?Influências de usinas hidrelétricas no funcionamento hidro-ecológico do Pantanal, Brasil? realizado durante o VIII INTECOL ? Conferência Internacional de Áreas Úmidas, em Cuiabá (20-25 de julho de 2008), que teve como objetivo embasar tecnicamente e de forma multidisciplinar a discussão sobre a conservação dos processos hidrológicos que regem o funcionamento e as inter-relações ecológicas características do Pantanal Mato-Grossense. O documento discute com propriedade a importância dos chamados ?pulsos de inundação?, ou ciclos de cheias e secas anuais e interanuais, os quais influenciam, por sua vez, as relações sociais, culturais e econômicas da população pantaneira. Este trabalho também mostra a preocupação dos participantes deste encontro científico com relação às intervenções previstas na bacia, que podem alterar o seu regime hidrológico, prejudicando a sustentabilidade da região.bitstream/CPAP-2010/57285/1/DOC102.pd

Urban stormwater modelling with MOHID

MOHID is a platform that includes a set of numerical models to simulate the water cycle in an integrated approach. It is an open source project that has been developed and applied to a wide range of studies since 1985. To increase its applicability for urban storm water modelling, the main module of the platform MOHID Land is now integrated with SWMM model via OpenMI. This poster evaluates the performance of MOHID in urban storm water modelling, by comparing results of the test cases presented by S. Néelz and G. Pender (2013) and of a real case study with InfoWorks ICM vs. 5.5. Moreover, it is discussed the advantage of covering the entire water cycle in MOHID platform, making it applicable for a wide range of cases

The seminal plasma proteins Peptidyl arginine deaminase 2, rRNA adenine N (6)-methyltransferase and KIAA0825 are linked to better motility post thaw in stallions

Seminal plasma plays an important role in sperm physiology. Seminal plasma proteins vehiculated in microvesicles, carry RNAs and proteins with a potential role in early embryo development. Additionally, proteins present in seminal plasma participate in redox regulation and energy metabolism. In view of these facts, we hypothesized that differences in protein composition of the seminal plasma among stallions may help to explain differences in freeze-ability seen among them. Three independent ejaculates from 10 different stallions of varying breeds were frozen using standard protocols in our laboratory. Aliquots of the ejaculate were separated and stored at −80 °C until further proteomic analysis. Semen analysis was performed using computer assisted sperm analysis and flow cytometry. Significant differences in proteome composition of seminal plasma were observed in the group of stallions showing better motility post thaw. 3116 proteins were identified, and of these, 34 were differentially expressed in stallions with better motility post thaw, 4 of them were also differentially expressed in stallions with different percentages of linearly motile sperm post thaw and 1 protein, Midasin, was expressed in stallions showing high circular velocity post thaw. Seminal plasma proteins may play a major role in sperm functionality; being vehiculated through extracellular vesicles and participating in sperm physiology. Bioinformatic analysis identifies discriminant proteins able to predict the outcome of cryopreservation, identifying potential new biomarkers to assess ejaculate quality

Seminal plasma AnnexinA2 protein is a relevant biomarker for stallions which require removal of seminal plasma for sperm survival upon refrigeration

Some stallions yield ejaculates that do not tolerate conservation by refrigeration prior to artificial insemination (AI), showing improvement after removal of most of the seminal plasma (SP) by centrifugation. In this study, the SP-proteome of 10 different stallions was defined through high-performance liquid chromatography with tandem mass spectrometry and bioinformatic analysis in relation to the ability of the ejaculates to maintain semen quality when cooled and stored at 5°C. Stallions were classified into three groups, depending on this ability: those maintaining good quality after direct extension in a commercial extender (good), stallions requiring removal of seminal plasma (RSP) to maintain seminal quality (good-RSP), and stallions, unable to maintain good semen quality even after RSP (poor). Pathway enrichment analysis of the proteins identified in whole equine SP using human orthologs was performed using g: profiler showing enriched Reactome and the Kyoto Encyclopedia of Genes and Genomes pathways related to hexose metabolism, vesicle mediated transport, post translational modification of proteins and immune response. Specific proteins overrepresented in stallions tolerating conservation by refrigeration included a peroxiredoxin-6 like protein, and transcobalamin-2, a primary vitamin B12-binding, and transport protein. Also, the protein involved in protein glycosylation, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 was present in good stallions. These proteins were nearly absent in poor stallions. Particularly, annexinA2 appeared as to be the most powerful discriminant variable for identification of stallions needing RSP prior to refrigeration, with a P = 0.002 and a q value = 0.005. Overall this is the first detailed study of the equine SP-proteome, showing the potential value of specific proteins as discriminant bio-markers for clinical classification of stallions for AI

DOT: A flexible multi-objective optimization framework for transferring features across single-cell and spatial omics

Single-cell RNA sequencing (scRNA-seq) and spatially-resolved imaging/sequencing technologies have revolutionized biomedical research. On one hand, scRNA-seq provides information about a large portion of the transcriptome for individual cells, but lacks the spatial context. On the other hand, spatially-resolved measurements come with a trade-off between resolution and gene coverage. Combining scRNA-seq with different spatially-resolved technologies can thus provide a more complete map of tissues with enhanced cellular resolution and gene coverage. Here, we propose DOT, a novel multi-objective optimization framework for transferring cellular features across these data modalities. DOT is flexible and can be used to infer categorical (cell type or cell state) or continuous features (gene expression) in different types of spatial omics. Our optimization model combines practical aspects related to tissue composition, technical effects, and integration of prior knowledge, thereby providing flexibility to combine scRNA-seq and both low- and high-resolution spatial data. Our fast implementation based on the Frank-Wolfe algorithm achieves state-of-the-art or improved performance in localizing cell features in high- and low-resolution spatial data and estimating the expression of unmeasured genes in low-coverage spatial data across different tissues. DOT is freely available and can be deployed efficiently without large computational resources; typical cases-studies can be run on a laptop, facilitating its use.Comment: 36 pages, 6 figure

Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility

Even in stallions with sperm quality within normal reference ranges at ejaculation, subtle differences in sperm quality exist that in many cases lead to reduced time frames for conservation of the ejaculate and/or reduced fertility. The spermatozoon is a cell highly suitable for proteomics studies, and the use of this technique is allowing rapid advances in the understanding of sperm biology. The aim of the present study was to investigate differences among stallions of variable sperm quality (based on motility and sperm velocities), although all horses had sperm characteristics within normal ranges. The proteome was studied using UHPLC/MS/MS and posterior bioinformatic and enrichment analysis; data are available via ProteomeXchange with identifier PXD025807. Sperm motility, linear motility and circular, straight line and average velocities (VCL, VSL, VAP) were measured using computer assisted sperm analysis (CASA). In stallions showing better percentages of motility, circular and average velocity predominated mitochondrial proteins with roles in the Citric acid cycle, pyruvate metabolism and oxidative phosphorylation. Interestingly, in stallions with better percentages of total motility, sperm proteins were also enriched in proteins within the gene ontology (G0) terms, single fertilization (G0: 0007338), fertilization (G0: 0009566), and zona pellucida receptor complex (GO:0002199). The enrichment of this proteins in samples with better percentages of total motility may offer a molecular explanation for the link between this parameter and fertility. Significance: Proteomic analysis identified a high degree of specificity of stallion sperm proteins with discriminant power for motility, linear motility, and sperm velocities (VCL, VAP and VSL). These findings may represent an interesting outcome in relation to the molecular biology regulating the movement of the spermatozoa, and the biological meaning of the measurements that computer assisted sperm analysis (CASA) provide. Of a total of 903 proteins identified in stallion spermatozoa, 24 were related to the percentage of total motility in the sample; interestingly, gene ontology (G0) analysis revealed that these proteins were enriched in terms like single fertilization and fertilization, providing a molecular link between motility and fertility. Field studies indicate that the percentage of total motility is the CASA derived parameter with the best correlation with fertility in stallions