Differential effect of valproate and its Delta(2)- and Delta(4)-unsaturated metabolites, on the beta-oxidation rate of long-chain and medium-chain fatty acids

Overall fatty acid oxidation rates were investigated in rat hepatocytes using [9,10-H-3]palmitic, [9,10-H-3]-oleic, [9.10-H-3]-myristic and [2,3-H-3]-phenylpropionic acids. The effect of both valproate (VPA) (0-10 mM) and two of its unsaturated metabolit

Synthesis and intramitochondrial levels of valproyl-coenzyme A metabolites

A number of valproate adverse reactions are due to its interference with several metabolic pathways, including that of fatty acid oxidation. In order to resolve which mitochondrial enzymes of fatty acid oxidation are inhibited by which VPA intermediates

cleavage of 3-oxovalproyl-CoA by a mitochondrial 3-oxoacyl-CoA thiolase

The beta-oxidation of valproic acid (VPA; 2-n-propylpentanoic acid) was investigated in vitro in intact rat liver mitochondria incubated with H-3-labelled VPA. The metabolism of [4,5-H-3(2)]VPA and [2-H-3]VPA was studied by analysing the different acyl-C

A postura de atletas de ginástica rítmica: análise através da fotometria

A postura apresenta relação com o tipo de modalidade esportiva praticada, acompanhada de características próprias. Por isso, este trabalho teve como objetivo verificar a postura e suas possíveis alterações em atletas de ginástica rítmica. Trata-se de um estudo de fotometria - fotografias retiradas de pontos específicos demarcados no corpo da atleta, sendo analisados pelo software de avaliação postural (SAPO). Participaram do estudo 27 indivíduos do sexo feminino, entre sete e 15 anos, atletas de uma equipe GR de Maringá, PR. Foram verificadas alterações em todas as atletas, sendo que algumas mostraram-se maiores, que podem ser justificadas pelos aspectos característicos da modalidade, e necessitam de novas análises. Portanto, a avaliação postural das atletas de GR torna-se importante como medida profilática para identificar os possíveis desalinhamentos posturais

Dekkera and Brettanomyces growth and utilisation of hydroxycinnamic acids in synthetic media

The original publication can be found at www.springerlink.comDekkera and Brettanomyces yeast are important spoilage organisms in a number of food and beverage products. Isolates of both genera were cultured in a defined medium and supplemented with hydroxycinnamic acids and vinylphenols to investigate their influence on growth and the formation of ethyl phenol derivatives. The growth rate of Brettanomyces species in the presence of acids was reduced, and no significant conversion to vinyl or ethyl derivatives was observed. The growth rate and substrate utilisation rates of Dekkera anomala and Dekkera bruxellensis yeast differed depending on strain and the acid precursor present. Growth of D. bruxellensis was slowed by the presence of ferulic acid with the addition of 1 mM ferulic acid completely inhibiting growth. This study provides an insight into the spoilage potential of these organisms and possible control strategies involving hydroxycinnamic acids.Victoria Harris, Christopher M. Ford, Vladimir Jiranek and Paul R. Grbi