A Critical Criminological Analysis of the Series “When they See us” and Some Considerations about the Institutional Racism

This work critically analyzes the series When they see us based on real facts made available on the Netflix digital platform This series was chosen for to a certain extent breaking with a white male and heterosexual hegemony reproduced in the series main on crime The series features the creator and director of the series Ava DuVernay a black woman The question to be answered by the work will be how does the series When they see us articulate as racial and gender categories in the illegal imprisonment of the five black adolescents It starts from the theoretical framework of critical criminology with racial class sex and gender cuts The technique used will be deductive with the exploratory methodology of bibliography The analysis carried out points to a central fact the racist selectivity of the American prison system The series managed to portray the current reality of the penal system both in the US and in Brazil and apply how it was strategically planned for the control of black bodies In addition to the selectivity of the system the series depicts the inhumane way that people deprived of their liberty are subjected and also how gender race and class structure this syste

NGS Panels applied to Hereditary Cancer Syndromes

Cancer is among the leading causes of morbidity and mortality worldwide (Okur et al, 2017). Germline pathogenic variants for monogenic, highly penetrant cancer susceptibility genes are observed in 5%–10% of all cancers (Lu et al, 2014). Hereditary cancers due to monogenic causes are characterized by earlier age of onset, other associated cancers, and often a family history of specific cancers. From the clinical perspective, it is important to recognize the affected individuals to provide them the best clinical management (Hennessy et al, 2010; Ledermann et al, 2014; Pennington et al, 2014) and to identify at-risk family members who will benefit from predictive genetic testing and enhanced surveillance, including early detection and/or risk reduction measures (Kurian et al, 2010; Okur et al, 2017). Germline variants identified in major cancer susceptibility genes associated with hereditary breast or ovarian cancer (HBOC) or hereditary colorectal cancer (HCRC), also account for 5-10% of the patients with these cancers. In the last years, new susceptibility genes, with different penetrance degrees, have been identified. Variants in any of those genes are rare and classical methodologies (e.g. Sanger sequencing - SS) are time consuming and expensive. Next-generation sequencing (NGS) has several advantages compared to SS, including the simultaneous analysis of many samples and sequencing of a large set of genes, higher sensitivity (down to 1% vs 15-20% in SS), lower cost and faster turnaround time, reasons that make NGS the best approach for molecular diagnosis. It is possible nowadays to choose between whole-genome sequencing (WGS), whole-exome sequencing (WES) and NGS limited to a set of genes (NGS-Panel). In cases where a suspected genetic disease or condition has been identified, targeted sequencing of specific genes or genomic regions is preferred (Grada et al, 2013). For that reason, we use NGS-Panel approach using TruSight Cancer (Illumina) to sequence DNA extracted from blood samples of patients with personal and/or familiar history of cancer. This hereditary cancer gene panel sequences 94 genes associated with both common (e.g., breast, colorectal) and rare hereditary cancers and allows the creation of virtual gene panels according to each phenotype or disease under study. NGS workflow analysis (Figure 1) includes five steps: quality assessment of raw data, read alignment to a reference genome, variant identification/calling, variant annotation and data visualization (Pabinger et al, 2013). The establishment of the most appropriate bioinformatics pipeline is crucial in order to achieve the best results. NGS data allows the identification of several types of variants like single nucleotide variants (SNVs), small insertions/deletions, inversions and also copy number variants (CNVs).FCT - UID/BIM/0009/2016info:eu-repo/semantics/publishedVersio

Proposal of smoothing space: disputes around the “modern Manaus”

AbstractThis article presents a reflection on projects for the so-called area of “Modern Manaus” in the city of Manaus/Amazonas, Brazil, which aim to create activities to define the uses of space as scenarios. This area stands as a space of dispute by various interested parties in the city of Manaus. The focus of this article lies with the changes proposed for the area of Modern Manaus and the issues experienced currently by this spatiality, for it is understood that pressures and class struggles are experienced in the city as a whole and, therefore, highlight the tensions of living in the city. Keywords: Manaus – City; Memories; History; Photos; Oral Sources. Original title: Propondo o alisamento do espaço: disputas em torno da “Manaus moderna