Desenvolvimento in vitro de bananeira 'Ouro' após poliploidização com antimitóticos

The objective of this work was to characterize the in vitro development of 'Ouro' banana after polyploidization with antimitotics. Shoot apexes were subjected to the following treatments for 24 and 48 hours: amiprophos-methyl (APM), at 0, 10, 20, 30, 40, and 60 µmol L-1; caffeine, at 3, 6, 9, and 12 g L-1; and colchicine, at 2.5 mmol L-1. Survival, number of shoots, main shoot height, and number of roots were evaluated. The intermediary concentrations were the most promising at both exposure times for APM and at 24 hours for caffeine. The highest concentrations of APM and caffeine negatively affect the in vitro development of the shoot apexes.O objetivo deste trabalho foi caracterizar o desenvolvimento in vitro de bananeira 'Ouro' após poliploidização com antimitóticos. Explantes foram submetidos aos seguintes tratamentos, por 24 e 48 horas: amiprofos-metil (APM), a 0, 10, 20, 30, 40 e 60 µmol L-1; cafeína, a 3, 6, 9 e 12 g L-1; e colchicina, a 2,5 mmol L-1. Avaliaram-se sobrevivência, número de brotos, altura do broto principal e número de raízes. As concentrações intermediárias foram as mais promissoras nos dois tempos de exposição, para APM, e no de 24 horas para cafeína. As maiores concentrações de APM e cafeína afetam negativamente o desenvolvimento in vitro dos explantes

Seroprevalence of Chikungunya virus and living conditions in Feira de Santana, Bahia-Brazil.

BACKGROUND: Chikungunya is an arbovirus, transmitted by Aedes mosquitoes, which emerged in the Americas in 2013 and spread rapidly to almost every country on this continent. In Brazil, where the first cases were detected in 2014, it currently has reached all regions of this country and more than 900,000 cases were reported. The clinical spectrum of chikungunya ranges from an acute self-limiting form to disabling chronic forms. The purpose of this study was to estimate the seroprevalence of chikungunya infection in a large Brazilian city and investigate the association between viral circulation and living condition. METHODOLOGY/PRINCIPAL FINDINGS: We conducted a population-based ecological study in selected Sentinel Areas (SA) through household interviews and a serologic survey in 2016/2017. The sample was of 1,981 individuals randomly selected. The CHIKV seroprevalence was 22.1% (17.1 IgG, 2.3 IgM, and 1.4 IgG and IgM) and varied between SA from 2.0% to 70.5%. The seroprevalence was significantly lower in SA with high living conditions compared to SA with low living condition. There was a positive association between CHIKV seroprevalence and population density (r = 0.2389; p = 0.02033). CONCLUSIONS/SIGNIFICANCE: The seroprevalence in this city was 2.6 times lower than the 57% observed in a study conducted in the epicentre of the CHIKV epidemic of this same urban centre. So, the herd immunity in this general population, after four years of circulation of this agent is relatively low. It indicates that CHIKV transmission may persist in that city, either in endemic form or in the form of a new epidemic, because the vector infestation is persistent. Besides, the significantly lower seroprevalences in SA of higher Living Condition suggest that beyond the surveillance of the disease, vector control and specific actions of basic sanitation, the reduction of the incidence of this infection also depends on the improvement of the general living conditions of the population

Polymorphisms in DENND1B gene are associated with asthma and atopy phenotypes in Brazilian children

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-28T19:15:53Z No. of bitstreams: 1 Fiuza BS Polymorphisms in DENND1B gene are associated....pdf: 785468 bytes, checksum: 752a3a4da4f9e5c39c215f56d208c14b (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-02-28T19:25:21Z (GMT) No. of bitstreams: 1 Fiuza BS Polymorphisms in DENND1B gene are associated....pdf: 785468 bytes, checksum: 752a3a4da4f9e5c39c215f56d208c14b (MD5)Made available in DSpace on 2018-02-28T19:25:21Z (GMT). No. of bitstreams: 1 Fiuza BS Polymorphisms in DENND1B gene are associated....pdf: 785468 bytes, checksum: 752a3a4da4f9e5c39c215f56d208c14b (MD5) Previous issue date: 2017CNPq − National Research Council - Brazil and FAPESB − State of Bahia, Brazil Research CouncilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, BrasilAsthma is a heterogeneous disease associated with a complex basis involving environmental factors and individual variabilities. The DENN Domain Containing 1B (DENND1B) gene has an important role on T cell receptor (TCR) down-regulation on Th2 cells and studies have shown that mutations or loss of this factor can be associated with increased Th2 responses and asthma. The aim of this work is to evaluate the association of polymorphisms in the DENND1B with asthma and allergy markers phenotypes in Brazilian children. Genotyping was performed using a commercial panel from Illumina (2.5 Human Omni bead chip) in 1309 participants of SCAALA (Social Change, Asthma, Allergy in Latin American) program. Logistic regressions for asthma and atopy markers were performed using PLINK software 1.9. The analyzes were adjusted for sex, age, helminth infections and ancestry markers. The DENND1B gene was associated with different phenotypes such as severe asthma and atopic markers (specific IgE production, skin prick test and IL-13 production). Among the 166 SNPs analyzed, 72 were associated with asthma and/or allergy markers. In conclusion, polymorphisms in the DENND1B are significantly associated with development of asthma and atopy and these polymorphisms can influence DENND1B expression and consequently, asthma

Food and nutrition insecurity: a marker of vulnerability to asthma symptoms.

OBJECTIVE: To evaluate the association between food and nutrition insecurity and asthma in children from Latin America. DESIGN: Cross-sectional study. SETTING: São Francisco do Conde, Bahia, north-eastern Brazil. SUBJECTS: The study included 1307 children aged 6-12 years from public elementary schools. Asthma symptoms were collected using a questionnaire that was translated and adapted from the International Study of Asthma and Allergies in Childhood, phase III. The diagnosis of asthma was determined based on reports of wheezing in the previous 12 months. The Brazilian Food Insecurity Scale was used to identify food insecurity. We also obtained demographic, socio-economic and anthropometric information for each participant. We used multivariate logistic regression analyses to assess the associations of interest. RESULTS: Of the children surveyed, 10·4% had a history of wheezing and 64·5% had some degree of food and nutrition insecurity. We found a positive dose-response relationship and statistically significant associations of asthma with moderate (OR = 1·71, 95% CI 1·01, 2·89) and severe (OR = 2·51, 95% CI 1·28, 4·93) food and nutrition insecurity. CONCLUSIONS: The results show that moderate and severe food and nutrition insecurity are markers of vulnerability to wheezing. It is important to note that the results of studies in this field have potential implications for social policies that promote food security. Further studies to identify the mechanisms involved in the relationship between food and nutrition insecurity and asthma are needed

Variants in the IL17 pathway genes are associated with atopic asthma and atopy makers in a South American population

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-09-27T18:49:19Z No. of bitstreams: 1 Silva, J. M. Variants_in_the_IL17_.pdf: 1261480 bytes, checksum: c76565f612715004421e85c96914b33a (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-09-27T18:59:41Z (GMT) No. of bitstreams: 1 Silva, J. M. Variants_in_the_IL17_.pdf: 1261480 bytes, checksum: c76565f612715004421e85c96914b33a (MD5)Made available in DSpace on 2019-09-27T18:59:41Z (GMT). No. of bitstreams: 1 Silva, J. M. Variants_in_the_IL17_.pdf: 1261480 bytes, checksum: c76565f612715004421e85c96914b33a (MD5) Previous issue date: 2019National Research Ethics Committee (reference number: 120.616) and free informed consent was properly obtained from the parents or legal guardian of each child.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Laboratório de Imunofarmacologia e Biologia Molecular. Departamento de Biorregulação. Salvador, BA, Brasil.Asthma is a complex disorder with multiple phenotypes which can influence its severity and response to treatment. The TH17 lymphocytes producing IL-17A and IL17-F cytokines, may have a role on asthma inflammation. The aim of our study was to evaluate the association between genetic variants in IL17 pathway genes with asthma and atopy markers. Materials and methods: Genotyping was performed using a commercial panel in 1245 participants of SCAALA cohort. The study included 91 SNVs in IL-17 pathway genes. Logistic regressions for asthma and atopy markers were performed using PLINK 1.9. In silico analyses were performed using rSNPbase, RegulomeDB, and Gtex portal for in silico gene expression. Results and discussion: The T allele of rs1974226 in IL17A was positively associated with asthma (OR: 1.37; 95% CI 1.02–1.82). Also, the T allele of rs279548 was positively associated with asthma (OR: 1.30; 95% CI 1.02–1.64), atopy (OR: 1.62; 95% CI 1.05–2.50) and increased expression of the IL17RC in lung and whole blood tissues. The others genetic variants in the IL17 pathways genes were associated with both protection and risk for asthma development as well as with IgE levels. Conclusion: The genetic variants in IL-17-related genes are associated with the atopic asthma phenotype and IgE production

Polymorphisms in the DAD1 and OXA1L genes are associated with asthma and atopy in a South American population

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-16T11:49:50Z No. of bitstreams: 1 Pires AO Polymorphisms in the DAD1 and OXA1L....pdf: 1226142 bytes, checksum: c0fa5af323611b005d0a4abe202c2c33 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-16T11:59:43Z (GMT) No. of bitstreams: 1 Pires AO Polymorphisms in the DAD1 and OXA1L....pdf: 1226142 bytes, checksum: c0fa5af323611b005d0a4abe202c2c33 (MD5)Made available in DSpace on 2018-08-16T11:59:43Z (GMT). No. of bitstreams: 1 Pires AO Polymorphisms in the DAD1 and OXA1L....pdf: 1226142 bytes, checksum: c0fa5af323611b005d0a4abe202c2c33 (MD5) Previous issue date: 2018Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo a Pesquisa do Estado da Bahia (FAPESB).Federal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Allergy and Acarology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Laboratory of Immunopharmacology and Molecular Biology. Salvador, BA, BrazilAtopic asthma, which is characterized by the chronic inflammation and morbidity of airways, is a disease of great complexity, and multiple genetic and environmental factors are involved in its etiology. In the first genome-wide association study (GWAS) conducted in Brazil for asthma, a positive association was found between atopic asthma and a variant (rs1999071), which is located between the DAD1 and OXA1L genes, although neither gene has previously been reported to be associated with asthma or allergies. The DAD1 gene is involved in the regulation of programmed cell death, and OXA1L is involved in biogenesis and mitochondrial oxidative phosphorylation. This study aimed to evaluate how polymorphisms in DAD1 and OXA1L are associated with asthma and markers of atopy in individuals from the Salvador cohort of the SCAALA (Social Change Asthma and Allergy in Latin America) program. The DNA of 1220 individuals was genotyped using the Illumina 2.5 Human Omni Bead chip. Logistic regression analyses were performed with PLINK 1.9 software to verify the association between DAD1 and OXA1L polymorphisms and asthma and atopic markers, adjusted for sex, age, helminth infections and ancestry markers, using an additive model. The DAD1 and OXA1L genes were associated with some of the evaluated phenotypes, such as asthma, skin prick test (SPT), specific IgE for aeroallergens, and Th1/Th2-type cytokine production. Using qPCR, as well as in silico gene expression analysis, we have demonstrated that some of the polymorphisms in both genes are able to affect their respective gene expression levels. In addition, DAD1 was over-expressed in asthmatic patients when compared with controls. Thus, our findings demonstrate that variants in both the DAD1 and OXA1L genes may affect atopy and asthma in a Latin American population with a high prevalence of asthma

Ocimum gratissimum Linn. and rosmarinic acid, attenuate eosinophilic airway inflammation in an experimental model of respiratory allergy to Blomia tropicalis.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-08-25T19:10:59Z No. of bitstreams: 1 Costa RS Ocimum gratissimum Linn ........pdf: 2216829 bytes, checksum: 513639b3e092aea34adaa12835404c4a (MD5)Made available in DSpace on 2014-08-25T19:10:59Z (GMT). No. of bitstreams: 1 Costa RS Ocimum gratissimum Linn ........pdf: 2216829 bytes, checksum: 513639b3e092aea34adaa12835404c4a (MD5) Previous issue date: 2012Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilUniversidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. Paraíba, JP, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilAllergic asthma has emerged as an important public health problem of urban populations in developed countries. Very often herbal medicine is used to treat this widespread disease, due to the lack of efficacy and the important side effects related to the classical drugs in use. Along this line, Ocimum gratissimum (Og) is a plant widely used in Brazilian folk medicine to treat inflammatory disorders, such as asthma. In the present study we evaluated the immunomodulatory effects of Og and rosmarinic acid (RA, a polyphenolic compound) in a murine model of respiratory allergy induced by the Blomia tropicalis (Bt) mite. The respiratory allergy was induced in A/J mice by administration of Bt extract and the treatment was done using 25, 50 or 100mg/kg of an Og methanolic extract or using 2, 20 or 200mg/kg of RA. We then evaluated the changes induced by these drugs on immunological parameters related to the allergic process, which are up-regulated in this allergic model. The treatment of animals with 100mg/Kg Og and 200mg/Kg RA led to a significant reduction in the numbers of leukocytes/eosinophils in bronchoalveolar lavage (BAL); eosinophil peroxidase activity in BAL; presence of mucus in respiratory tract, histopathological changes in the lung, and IL-4 in BAL. These results suggest that the methanolic extract of Og and the polyphenol RA have therapeutic potential in this murine model of respiratory allergy to a clinically relevant human sensitizer allergen

Seroprevalence of Chikungunya virus and living conditions in Feira de Santana, Bahia-Brazil. S1 Database

Chikungunya is an arbovirus, transmitted by Aedes mosquitoes, which emerged in the Americas in 2013 and spread rapidly to almost every country on this continent. In Brazil, where the first cases were detected in 2014, it currently has reached all regions of this country and more than 900,000 cases were reported. The clinical spectrum of chikungunya ranges from an acute self-limiting form to disabling chronic forms. The purpose of this study was to estimate the seroprevalence of chikungunya infection in a large Brazilian city and investigate the association between viral circulation and living condition