Preliminary observations on the anopheline mosquitoes of gomadiyagala a village in the north western province of Sri Lanka

Investigations on anopheline mosquitoes were carried out in Gomadiyagaladuring January-December 1989, primarily to gather base-line data in relationtoa collaborative study between the Entomology Division, Anti-Malaria Campaignand the Department of Zoology, University of Sri Jayewardenepura on thepotential for a genetic approach for malaria control in Sri Lanka. Eight anophelinespecies were recordedfrom adult catches and lavae sampled. An. subpictusAn. culicifacies and An. varuna were the most predominant species.The only stream with two tributaries in the study area was the permanentand very productive anopheline breeding habitat. Larval and adult anophelinedensities were related to the rainfall received. The larval densities recorded indrought periods were observed to be significantly higher than those recorded inrainy periods (t=4.087, p<O.Ol). August, September and October that recordedrelatively low larval and adult An. culicifacies densities appear to be suitabletimes for field releases of genetically altered An.culicifacies in the area for malariacontrol

Prenatal diagnosis of mosaic ring chromosome 16 - a rare event with uncertain prognosis

Ring chromosomes are rare cytogenetic findings (prenatal frequency ~ 0.0075%) often associated with an abnormal phenotype, depending of the chromosomal origin, genetic content and the presence of a mosaic. Supernumerary ring chromosome 16 [r(16)] is rarely observed and mosaicism makes the genotype/phenotype correlation difficult. We report a de novo mosaic r(16) detected after prenatal diagnosis in a woman referred for advanced maternal age. Multiplex ligation-dependent probe amplification (MLPA) for aneuploidy testing of chromosomes 13, 18, 21 and X was normal. Karyotype was 47,XX,+r[10]/46,XX[15]. Chromosomal microarray analysis (CMA) on DNA obtained from long-term cultured amniocytes did not detect any alterations. MLPA with a pericentromeric probe kit on an uncultured sample showed a chromosome 16 gain, encompassing 16p11.2 and 16q11.2 regions, including TGFB1I1, AHSP, VPS35 and ORC6 genes, leading to partial characterization of the r(16). Although no phenotype has been correlated with overexpression of these genes, the 16p11.2 region is associated with neurodevelopmental disorders. Nevertheless individuals with microduplication of 16p11.2 and normal development have been described. The lack of a precise definition of genetic content of the r(16) and its mosaic form leads to uncertain prognosis of clinical outcome.N/

Cytokine Production by Human Colonic Intraepithelial Lymphocytes in Controls and Ulcerative Colitis

Using an ELISA technique, concentrations of γ-interferon and interleukin-2 were assayed in the supernatants of colonic intraepithelial lymphocytes cultured with or without phytohaemagglutinin (PHA). IntraepitheHal lymphocytes produced low concentrations of γ-interferon and interleukin-2 when stimulated with PHA, but significantly more than when unstimulated (p<0.05). There was no difference in production of these cytokines by IEL from control or inflammatory bowel disease

Dose effects in He implanted Eurofer97 steel

Reduced Activation Ferritic/Martensitic steels are being extensively studied because of their foreseen application in fusion reactors. To reproduce neutron irradiation conditions, Eurofer97 samples were implanted at room temperature with helium ions at energies of 500 keV and 2 MeV and doses of 1x1015-1017 He /cm2. The implantation induced defects were characterized by positron beam Doppler Broadening (DB). The samples were annealed in the range 300 – 1500 K, in 100 K steps. As the temperature increases, the annealing of vacancies and vacancy clusters is noticed and followed by the coalescence of HenVm clusters. At temperatures around 1200 K HeV pairs dissociate and bubbles are formed. Above 1300 K the helium release from bubbles is observed. The S-W graphs reveals that the samples have similar positron traps up to 1200 K. At 1200 K helium bubbles are noticed and the S,W pair shows a clearly distinct behaviour from the S,W values of vacancy type defects. As the temperature increases and helium is release, the S,W pairs shift towards the S,W’s of vacancies.RST/Radiation, Science and TechnologyApplied Science

Optimization on manifolds : methods and applications

Summary. This paper provides an introduction to the topic of optimization on manifolds. The approach taken uses the language of differential geometry, however, we choose to emphasise the intuition of the concepts and the structures that are important in generating practical numerical algorithms rather than the technical details of the formulation. There are a number of algorithms that can be applied to solve such problems and we discuss the steepest descent and Newton’s method in some detail as well as referencing the more important of the other approaches. There are a wide range of potential applications that we are aware of, and we briefly discuss these applications, as well as explaining one or two in more detail

Effects of He+ ion implantation on surface properties of UV-cured Bis-GMA/TEGDMA bio-compatible resins

This work reports on the surface characterisation of 2,2-bis[4-(2-hydroxy- 3-methacryloxyl-oxypropoxy)phenyl]propane/triethylene glycol dimethacrylate bio-compatible resins after high energy He+ ion implantation treatments. The samples have been characterised by diffuse reflectance FT-IR, X-ray photo-electron spectroscopy, ultramicro-hardness and nano-scratch wear tests. In addition, osteblast cell assays MG-63 have been used to test the bio-compatibility of the resin surfaces after the ion implantation treatments. It has been observed that the maximum surface hardening of the resin surfaces is achieved at He-ion implantation energies of around 50 keV and fluences of 1 7 1016 cm-2. At 50 keV of He-ion bombardment, the wear rate of the resin surface decreases by a factor 2 with respect to the pristine resin. Finally, in vitro tests indicate that the He-ion implantation does not affect to the cell-proliferation behaviour of the UV-cured resins. The enhancement of the surface mechanical properties of these materials can have beneficial consequences, for instance in preventing wear and surface fatigue of bone-fixation prostheses, whose surfaces are continuously held to sliding and shearing contacts of sub-millimetre scale lengths. \ua9 2010 Elsevier B.V. All rights reserved