Conocimientos y actitudes sobre VIH/SIDA en el personal de Salud del HOSPITAL VICENTE CORRAL MOSCOSO. 2016

ANTECEDENTES: Junto con la aparición del VIH también surgió la discriminación a las personas que la padecen. La sociedad en sí, manifiesta un tipo de separación social hacia estas personas a pesar de que muchas veces, tienen cierto conocimiento sobre el comportamiento de esta enfermedad. OBJETIVO: Determinar los conocimientos y actitudes que tiene el personal de salud sobre VIH/SIDA, en el Hospital Vicente Corral Moscoso en el año 2016. METODOLOGÍA: Este es un estudio cualitativo - descriptivo de corte transversal dirigido a todo el personal de salud del Hospital Vicente Corral Moscoso empleando una muestra de 279 personas. La recolección de la información se realizó por medio de cuestionarios, VIH-65 y Escala Multicomponente de Fobia al Sida. Se creó una base de datos en el programa SPSS versión 19.0. RESULTADOS: Los conocimientos tanto los correctos como los erróneos sobre VIH/SIDA son muy altos, la media fue superior al 95% de respuestas correctas. Existen conductas estigmatizantes en relación con el SIDA. CONCLUSIONES: Los resultados mostraron que a pesar de que los conocimientos son altos sobre el VIH/SIDA existen conductas de discriminación y fobia poco esperadas por parte del personal de la saludBACKGROUND: Along with the onset of HIV, discrimination has also arisen for people who have it. The society itself, shows repudiation towards these people although they often have some knowledge about the behavior of this disease. OBJECTIVE: To determine the knowledge and attitudes that health personnel have about HIV / AIDS, at the Hospital Vicente Corral Moscoso in 2016. METHODOLOGY: This is a qualitative - descriptive cross-sectional study directed at all health personnel of the Hospital Vicente Corral Moscoso employing a sample of 279 people. The information was collected through questionnaires, HIV-65 and Multicomponent Scale of Phobia to AIDS. A database was created in the SPSS software version 19.0. RESULTS: Both correct and erroneous knowledge about HIV / AIDS are very high, the mean was above 95% correct answers. Positive, negative attitudes and self-efficacy showed high values. There are stigmatizing behaviors in relation to AIDS. CONCLUSIONS: The results showed that although knowledge is high on HIV / AIDS, there are unpredictable discrimination and phobia behaviors among health personnel.MédicoCuenc

Complete Genome Sequence of an Escherichia coli Strain Isolated from Laboratory Mouse Stool for Use as a Chassis for Transgene Delivery to the Murine Microbiome

Tools to explore functional changes in the microbiome are limited. Here, we report the complete genome sequence of a strain of Escherichia coli that was isolated from murine stool. This sequence will provide essential information to further develop this tool, and similar tools, to explore the complex murine microbiome

Intestinal transgene delivery with native E. coli chassis allows persistent physiological changes.

Live bacterial therapeutics (LBTs) could reverse diseases by engrafting in the gut and providing persistent beneficial functions in the host. However, attempts to functionally manipulate the gut microbiome of conventionally raised (CR) hosts have been unsuccessful because engineered microbial organisms (i.e., chassis) have difficulty in colonizing the hostile luminal environment. In this proof-of-concept study, we use native bacteria as chassis for transgene delivery to impact CR host physiology. Native Escherichia coli bacteria isolated from the stool cultures of CR mice were modified to express functional genes. The reintroduction of these strains induces perpetual engraftment in the intestine. In addition, engineered native E. coli can induce functional changes that affect physiology of and reverse pathology in CR hosts months after administration. Thus, using native bacteria as chassis to "knock in" specific functions allows mechanistic studies of specific microbial activities in the microbiome of CR hosts and enables LBT with curative intent