Large area in situ fabrication of Poly(pyrrole)-nanowires on flexible thermoplastic films using Nanocontact printing

Abstract: Highly efficient nano-engineering tools will certainly revolutionize the biomedical and sensing devices research and development in the years to come. Here, we present a novel high performance conducting poly(pyrrole) nanowires (PPy-NW) patterning technology on thermoplastic surfaces (poly(ethylene terephthalate (PETE), poly(ethylene 2,6-naphthalate (PEN), polyimide (PI), and cyclic olefin copolymer (COC)) using nanocontact printing and controlled chemical polymerization (nCP-CCP) technique. The technique uses a commercial compact disk (CD) as a template to produce nanopatterned polydimethylsiloxane (PDMS) stamps. The PDMS nanopatterned stamp was applied to print the PPy-NWs and the developed technology of nCP-CCP produced 3D conducting nanostructures. This new and very promising nanopatterning technology was achieved in a single step and with a low cost of fabrication over large areas

Mise au point de capteurs économiques pour la détermination électroanalytique des ions nitrate ou nitrite dans les eaux naturelles

Les ions nitrate et les ions nitrite sont des composés chimiques présents naturellement dans l'environnement. Normalement leur taux dans les eaux superficielles et dans les eaux souterraines est faible. Il peut cependant atteindre des niveaux élevés suite à certaines activités humaines et représenter un risque sanitaire et environnemental. La mise au point de moyens peu coûteux permettant de surveiller le taux des ions nitrate et nitrite dans les eaux de surface est une nécessité pour la préservation de l'environnement et de la santé humaine. Face à cette nécessité nous nous sommes intéressés à l étude de matériaux relativement facile à mettre en oeuvre et d un coût modéré. Pour les ions nitrate, nous nous sommes intéressés au cuivre, au palladium et aux films de cuivre sur disque de palladium. Ces électrodes ont été testées sur des eaux synthétiques et des eaux naturelles de surface. Les réponses de ces matériaux, obtenues par chronoampérométrie, lors des mesures en continu des ions nitrate dans ces milieux montrent que le disque de palladium est le matériau le mieux adapté à ce type de mesure. Le palladium est donc choisi pour la mise en place d un mini système à trois électrodes solides enchâssées dans une résine. Les tests de surveillance des niveaux de pollutions en ions nitrate d une eau naturelle circulant dans une rivière artificielle confirme que ce mini système peut servir de capteur de surveillance des niveaux d ions nitrate dans les eaux naturelles de surface. Pour la mesure des ions nitrite, le matériau étudié est un film de cobalt électrodéposé sur disque de cuivre. Il est montré dans ce travail que ce matériau peut servir d électrode pour la mesure par ajouts dosés des ions nitrite dans des échantillons d eau naturelle. Cette électrode atteint une limite de détection de 9.10-3 ppm soit 2.10-7 mol/L d ions nitrite sans interférence des ions nitrate présents en quantité importante dans l eau naturelle. Pour les ions nitrate et les ions nitrite, il est également montré que la présence des ions hydrogénocarbonate, sulfate, chlorure, calcium, magnésium n empêche pas leur détection à l aide des capteurs, aux teneurs usuelles rencontrées dans les eaux souterraines et de surfaceLYON1-BU.Sciences (692662101) / SudocSudocFranceF

A fully integrated electrochemical biosensor platform fabrication process for cytokines detection

International audienceInterleuldn-1b (IL-1b) and interleukin-10 (IL-10) biomarkers are one of many antigens that are secreted in acute stages of inflammation after left ventricle assisted device (LVAD) implantation for patients suffering from heart failure (HF). In the present study, we have developed a fully integrated electrochemical biosensor platform for cytokine detection at minute concentrations. Using eight gold working microelectrodes (WEs) the design will increase the sensitivity of detection, decrease the time of measurements, and allow a simultaneous detection of varying cytokine biomarkers. The biosensor platform was fabricated onto silicon substrates using silicon technology. Monoclonal antibodies (mAb) of anti-human IL-lb and anti-human IL-10 were electroaddressed onto the gold WEs through functionalization with 4-carboxymethyl aryl diazonium (CMA). Cyclic voltammetry (CV) was applied during the WE functionalization process to characterize the gold WE surface properties. Finally, electrochemical impedance spectroscopy (EIS) characterized the modified gold WE. The biosensor platform was highly sensitive to the corresponding cytokines and no interference with other cytokines was observed. Both cytokines: IL-10 and IL-lb were detected within the range of 1 pg mL(-1) to 15 pg mL(-1). The present electrochemical biosensor platform is very promising for multi-detection of biomolecules which can dramatically decrease the time of analysis. This can provide data to clinicians and doctors concerning cytokines secretion at minute concentrations and the prediction of the first signs of inflammation after LVAD implantation

Methylene blue phosphoramidite for DNA labelling

We thank Oceane Clabaux and Alexandre Escoffier for their contribution to the work.International audienceWe report the first synthesis of a methylene blue (MB) phosphoramidite derivative suitable for DNA solid-phase synthesis. The electrochemical and optical properties of the resulting MB modified oligonucleotides were confirmed. This new molecule is an important breakthrough in the design of new probes labelled with MB

Poly(pyrrole) microwires fabrication process on flexible thermoplastics polymers: application as a biosensing material

The authors would like to thank to Carole FARRE (Institute of Analytical Sciences, Univ. Lyon I) for the optical microscopy guidanceInternational audienceWe present a new low-cost technology to fabricate poly(pyrrole) microwires (PPy-mu Ws)by micro contact printing (mu CP) and controlled chemical polymerization (mu CP-CCP) using three different techniques ("grafting printing", "addressed printing", and "direct printing". These developed techniques provide a platform to regulate PPy wire size and shape using N-(3-trimethoxysilyl-propyl) pyrrole (Py-silane) for the "addressed printing" and "direct printing" techniques, respectively. The technique uses a silicon mold to produce micropatterned polydimethylsiloxane (PDMS) stamps. The micropatterned PDMS stamp was employed to print PPy-mu Ws with an improvement observed from "direct printing" > "addressed printing">"grafting printing". Therefore, for "direct printing", PPy-mu Ws were printed on: poly(ethylene terephthalate) (PETE), cyclic olefin copolymer (COC), poly(ethylene 2,6-naphthalate (PEN), and polyimide (PI) surfaces. Here, we have studied the printing of wires and the surface morphology to understand the characteristics of PPy patterns by optical microscopy, scanning electrode microscopy (SEM), and atomic force microscopy (AFM). The functionality of the PPy-mu W matrix was also analyzed for immune detection. Here, fluorescent imaging was used to certify the detection of recombinant human interleukin-10 (rh IL-10). PPy-mu Ws were functionalized using gluteraldehyde (GA) for the specific immobilization of antihuman interleukin-10 monoclonal antibodies (anti-IL-10 mAb's). The immunosensor present a sensitivity of 0.026 (pg/mL(-1)) to the respective rh IL-10 in a linear range of 1-50 pg/mL and a limit of detection at 0.36 pg/L. Finally the PPy-mu W matrix was successfully tested for cytokine bioassays using electrochemical impedance spectroscopy (EIS) and presents outstanding properties as a matrix for biosensing

Energy harvesting from river sediment using a biofuel cell: preliminary tests

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Extraction d'énergie en rivière à l'aide d'une biopile sédimentaire : essais préliminaires

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Energy harvesting from river sediment using a biofuel cell: preliminary tests

International audienc

Amperometric quantification based on serial dilution microfluidic systems

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Controlled poly(pyrrole) patterning by microcontact printing on glass and poly(ethylene terephthalate) substrates

International audienceIn this work, we present an easy and low-cost technique for the fabrication of poly(pyrrole) (PPy) micro structures onto glass and poly(ethylene naphthalate) (PET) substrates by combining microcontact printing (mu CP) and electroless polymerization. The substrates: glass and PET were activated in piranha and potassium hydroxide (KOH) solution, respectively. The activated substrates were then functionalized with 11-(triethoxysilyl)undecanal (TESUD) using structured polydimethylsiloxane (PDMS) stamps and transferred by mu CP. After printing, the PPy was grown onto the silane patterned surface using electroless polymerization. The printed patterns were characterized by optical microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The results have shown that PPy rings can be developed and were well-defined onto glass and PET substrates through mu CP and electroless polymerization. The PPy was grown to dimensions of an inner ring diameter (IRD) of 50, 30 and 20 mu m and an outer ring diameter CORD) of 30, 20 and 10 mu m, respectively. The estimated thickness of PPy structures was measured at 2.8 +/- 0.4 mu m by AFM. This innovating technique represents a first step in the production of a PPy printed matrix for potential flexible biosensors