Sperm competition in the scorpionfly <i>Panorpa communis</i> (Mecoptera, Insecta)

The aim of the present study was to investigate the basal mechanism of sperm competition in the European scorpionfly Panorpa communis. Within three laboratory experiments, I used five newly developed microsatellite markers to detect the mechanism of sperm competition in the scorpionfly P. communis. In general, sperm competition occurs if sperm of different males compete for the fertilisation of the ova of one female. As P. communis females mate multiple with different males and store sperm in a storage organ until fertilisation, there is sperm competition in this species. To clarify the outcome of sperm competition, paternity detection of offspring of a multiple mated female is necessary. The most practicable way for paternity detection is the application of microsatellite markers. As a first step of my work, I established five new markers to achieve species specific microsatellites for P. communis. Then, I arranged two experiments in order to detect the mechanism of sperm competition, Here, females were paired to two or three different males, respectively. Since copulation duration, generally, is known to be a good estimator for the number of transferred sperm, I was able to draw conclusions from the proportion of sperm a male contributed to the spermatheca of the female in relation to rival males. I was able to show that the outcome of sperm competition is not influenced by the mating order of males. Consequently, any form of last male sperm precedence for P. communis could be excluded. But, paternity patterns were influenced by copulation duration and therefore by the proportion of sperm represented in the spermatheca. Both experiments conclude that, in P. communis, sperm of different males is mixed and compete numerically for fertilisations, i.e. there is a fair raffle in sperm competition. In a further experiment, I analysed if sperm transfer rates of different males are equal. Although, males were slightly different respective their sperm transfer rates, these differences did not influence the outcome of sperm competition. Accordingly, using copulation duration as a general estimator for the number of transferred sperm is possible. Finally, I discussed the role of sperm competition for scorpionflies in general and, how it may be maintained in this group. Furthermore, I hypothesised how the remarkable mating system in combination with different sperm competition mechanisms in scorpionflies may have evolved

Comparison of volatile compounds and sensory profiles of alcoholic black currant (Ribes nigrum) beverages produced with Saccharomyces, Torulaspora, and Metschnikowia yeasts

Black currants (Ribes nigrum) were fermented with Saccharomyces and non-Saccharomyces yeasts without added sugar to yield low-ethanol-content beverages. The effects of yeasts on the volatile compounds and sensory characteristics were analysed by HS-SPME-GC-MS, GC-O, and generic descriptive analysis. Ninety-eight volatile compounds were identified from the black currant juice and fermented beverages. Significant increases in the contents of esters (131 %), higher alcohols (391 %), and fatty acids (not present in juice sample) compared to initial juice were observed depending on the yeasts used.  GC-O analysis revealed the higher impact of esters on the sensory properties of Saccharomyces bayanus-fermented beverage compared to the Torulaspora delbrueckii-fermented beverage. In the sensory evaluation, non-Saccharomyces yeasts resulted in a higher ‘black currant odour’. However, all beverages were intensely sour, which can be a significant challenge in the development of alcoholic berry beverages.</p

D-cycloserine augmentation of exposure-based cognitive behavior therapy for anxiety, obsessive-compulsive, and posttraumatic stress disorders: a systematic review and meta-analysis of individual participant data

Importance: Whether and under which conditions D-cycloserine (DCS) augments the effects of exposure-based cognitive behavior therapy for anxiety, obsessive-compulsive, and posttraumatic stress disorders is unclear. Objective: To clarify whether DCS is superior to placebo in augmenting the effects of cognitive behavior therapy for anxiety, obsessive-compulsive, and posttraumatic stress disorders and to evaluate whether antidepressants interact with DCS and the effect of potential moderating variables. Data Sources: PubMed, EMBASE, and PsycINFO were searched from inception to February 10, 2016. Reference lists of previous reviews and meta-analyses and reports of randomized clinical trials were also checked. Study Selection: Studies were eligible for inclusion if they were (1) double-blind randomized clinical trials of DCS as an augmentation strategy for exposure-based cognitive behavior therapy and (2) conducted in humans diagnosed as having specific phobia, social anxiety disorder, panic disorder with or without agoraphobia, obsessive-compulsive disorder, or posttraumatic stress disorder. Data Extraction and Synthesis: Raw data were obtained from the authors and quality controlled. Data were ranked to ensure a consistent metric across studies (score range, 0-100). We used a 3-level multilevel model nesting repeated measures of outcomes within participants, who were nested within studies. Results: Individual participant data were obtained for 21 of 22 eligible trials, representing 1047 of 1073 eligible participants. When controlling for antidepressant use, participants receiving DCS showed greater improvement from pretreatment to posttreatment (mean difference, -3.62; 95% CI, -0.81 to -6.43; P = .01; d = -0.25) but not from pretreatment to midtreatment (mean difference, -1.66; 95% CI, -4.92 to 1.60; P = .32; d = -0.14) or from pretreatment to follow-up (mean difference, -2.98, 95% CI, -5.99 to 0.03; P = .05; d = -0.19). Additional analyses showed that participants assigned to DCS were associated with lower symptom severity than those assigned to placebo at posttreatment and at follow-up. Antidepressants did not moderate the effects of DCS. None of the prespecified patient-level or study-level moderators was associated with outcomes. Conclusions and Relevance: D-cycloserine is associated with a small augmentation effect on exposure-based therapy. This effect is not moderated by the concurrent use of antidepressants. Further research is needed to identify patient and/or therapy characteristics associated with DCS response.2018-05-0

In Situ Prior Proliferation of CD4+ CCR6+ Regulatory T Cells Facilitated by TGF-β Secreting DCs Is Crucial for Their Enrichment and Suppression in Tumor Immunity

BACKGROUND: CD4(+)CD25(+) regulatory T cells (Tregs), a heterogeneous population, were enrichment in tumor mass and played an important role in modulating anti-tumor immunity. Recently, we reported a Treg subset, CCR6(+) Tregs but not CCR6(-)Tregs, were enriched in tumor mass and closely related to poor prognosis of breast cancer patients. However, the underlying mechanism remains elusive. Here, we carefully evaluate the enrichment of CCR6(+)Tregs in tumor mass during progression of breast cancer and explore its possible mechanism. METHODOLOGY/PRINCIPAL FINDINGS: The frequency of CCR6(+)Tregs in tumor infiltrating lymphocytes (TILs ) was analyzed at early stage and at late stage of tumor in a murine breast cancer model by FACS respectively. The expansion of CCR6(+)Tregs and their CCR6(-) counterpart in tumor mass were determined by BrdU incorporation assay. The effect and its possible mechanism of tumor-resident antigen presenting cells (APCs) on the proliferation of CCR6(+)Tregs also were evaluated. The role of local expansion of CCR6(+)Tregs in their enrichment and suppression in vivo also was evaluated in adoptive cell transfer assay. We found that the prior enrichment of CCR6(+)Tregs but not CCR6(-)Tregs in tumor mass during progression of murine breast cancer, which was dependent on the dominant proliferation of CCR6(+) Tregs in situ. Further study demonstrated that tumor-resident DCs triggered the proliferation of CCR6(+)Treg cells in TGF-β dependent manner. Adoptive transfer of CCR6(+)Tregs was found to potently inhibit the function of CD8(+)T cells in vivo, which was dependent on their proliferation and subsequently enrichment in tumor mass. CONCLUSIONS/SIGNIFICANCE: Our finding suggested that CCR6(+) Tregs, a distinct subset of Tregs, exert its predominant suppressive role in tumor immunity through prior in situ expansion, which might ultimately provide helpful thoughts for the designing of Treg-based immunotherapy for tumor in the future

A cross-cultural study on odor-elicited life stage-associations

Associative conceptualization plays an important role in how we perceive and interact with our environment. Particularly odor associations can be highly vivid and often long-lasting due to their close connection with our episodic memory and emotions. Based on the findings of a study conducted in Austria in 2017, this work was carried out to investigate odor-elicited life stage-associations (OELSA) in seven nations and to identify potential similarities and differences in conceptualizing odor impressions across these nations. A total of 1144 adults (aged 21–60) from Austria, Australia, Germany, Switzerland, Thailand, USA, and Vietnam participated in this study. Nine odors (vanilla, orange, lemon, mint, coconut, basil, rose, anise, and hay) were presented to the participants, and they were asked to answer questions about their spontaneous associations with life stages. The results indicate the existence of OELSA in all investigated nations. For example, vanilla was predominantly assigned to children in all nations, while hay was primarily assigned to elder people. While most of the investigated odors were most frequently associated with adults, some significant differences in OELSA were observed between the different nationalities. For instance, mint was more frequently associated with children by Australian participants compared to participants from all other nations, while coconut was more strongly associated with children by the Vietnamese participants compared to all other participants. The results of this study demonstrate the existence of consistent life stage-related associations elicited by certain odors across different nations and cultures and, at the same time points to differences in life stage-related association with certain odors between the nations. Since this work was not designed to identify the reasons for these differences, we can only make assumptions about the potential underlying causes for these behaviors

Empirical Standards for Software Engineering Research

Empirical Standards are natural-language models of a scientific community's expectations for a specific kind of study (e.g. a questionnaire survey). The ACM SIGSOFT Paper and Peer Review Quality Initiative generated empirical standards for research methods commonly used in software engineering. These living documents, which should be continuously revised to reflect evolving consensus around research best practices, will improve research quality and make peer review more effective, reliable, transparent and fair.Comment: For the complete standards, supplements and other resources, see https://github.com/acmsigsoft/EmpiricalStandard

Coronavirus-Pandemie: Wirksame Regeln für Herbst und Winter aufstellen : 6. Ad-hoc-Stellungnahme - 23. September 2020

Wirksame Regeln für Herbst und Winter zur Bekämpfung der Pandemie: Schutzmaßnahmen konsequent einhalten, schnell und gezielt testen, Quarantäne- und Isolationszeiten verkürzen, verantwortungsvolles Handeln erleichtern, soziale und psychische Folgen abmildern