Multiparameter correlation microscopy of blood plasma polycrystalline networks in the diagnosis of cancer tissues of female reproductive system

The principles of optical model of human bile polycrystalline structure are described. The results of investigating the interrelation between the values of statistical, correlation and fractal parameters are presented. They characterize the coordinate distributions of mutual polarization degree of the points of laser images of bile smears of cholelithiasis patients in combination with other pathologies. The diagnostic criteria of the cholelithiasis nascency and its severity degree differentiation are determined

Multiparameter correlation microscopy of blood plasma polycrystalline networks in the diagnosis of cancer tissues of female reproductive system

The principles of optical model of human bile polycrystalline structure are described. The results of investigating the interrelation between the values of statistical, correlation and fractal parameters are presented. They characterize the coordinate distributions of mutual polarization degree of the points of laser images of bile smears of cholelithiasis patients in combination with other pathologies. The diagnostic criteria of the cholelithiasis nascency and its severity degree differentiation are determined

Azimuthally invariant phase tomography of optically anisotropic sections of biological tissues

Considered in this work is the theoretical background of the azimuthally stable method of Jones matrix mapping applied to histological sections of uterine neck biopsy based on spatial-frequency selection of the mechanisms responsible for linear and circular irefringence. The comparative results of measuring the coordinate distributions of the complex degree of mutual anisotropy formed by fibrillar networks of myosin and collagen fibrils of uterine neck tissue being in different pathological states – pre-cancer (dysplasia) and cancer (adenocarcinoma) – have been shown. The values and ranges of the change in the statistical (moments of the 1st to 4 th orders) parameters of the complex degree of mutual anisotropy of coordinate distributions have been studied. The objective criteria for diagnostics of pathology and differentiation of its severity degree have been determined

Statistical and fractal analyses of autofluorescence of myocardium tissue images in diagnostics of acute coronary insufficiency

This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections of the myocardium). The polarized structure of autofluorescence imaging layers of biological tissues was detected and investigated. Proposed in this work is the model for describing formation of polarization inhomogeneity in autofluorescent images of biological optically anisotropic layers. On its basis, analytically and experimentally tested is the method of laser autofluorescent polarimetry. Analyzed has been the effectiveness of this method in the postmortem diagnosis of infarction. The objective criteria (statistical moments) of differentiation of autofluorescent images of histological sections myocardium have been determined. The operational characteristics (sensitivity, specificity, accuracy) of this technique have been ascertained

LASER POLARIZATION-VARIABLE AUTOFLUORESCENCE OF THE NETWORK OF OPTICALLY ANISOTROPIC BIOLOGICAL TISSUES: DIAGNOSTICS AND DIFFERENTIATION OF EARLY STAGES OF CANCER OF CERVIX UTERI

This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears) in the task of diagnostics and differentiation of early stages of cancer: Dysplasia — cervical microinvasive carcinoma of cervix uteri. The analytical conditions of polarization-optimal probing of biological layers were determined basing on the model of linear birefringence and dichroism of birefringent (fibrillar, porphyrin) networks. The technique of polarization-variable laser autofluorescence was developed and experimentally tested. The objective criteria (statistical moments) of differentiation of histological sections autofluorescent images of endometrium biopsy and cytological smears of it mucous coat were defined. The operational characteristics (sensitivity, specificity, accuracy) of this technique were determined concerning the positions of probative medicine, and clinical efficiency

The singular approach for processing polarization-inhomogeneous laser images of blood plasma layers

We present in this work the results of an investigation to analyse the coordinate distributions of azimuths and ellipticity of polarization (polarization maps) in laser images of blood plasma layers for three groups of patients: healthy (group 1), mastopathy (group 2) and breast cancer (group 3). To characterize polarization maps for all groups of samples we use three groups of parameters: statistical moments of the first to fourth orders, autocorrelation functions and logarithmic dependences for power spectra related to distributions of azimuths and ellipticity of polarization inherent to laser images of blood plasma. We ascertain the criteria for diagnosis and differentiation of pathological changes of the breast

System of multifunctional Jones matrix tomography of phase anisotropy in diagnostics of endometriosis

The paper presents the results of Jones-matrix mapping of uterine wall histological sections with second-degree and third-degree endometriosis. The technique of experimental measurement of coordinate distributions of the modulus and phase values of Jones matrix elements is suggested. Within the statistical and cross-correlation approaches the modulus and phase maps of Jones matrix images of optically thin biological layers of polycrystalline films of plasma and cerebrospinal fluid are analyzed. A set of objective parameters (statistical and generalized correlation moments), which are the most sensitive to changes in the phase of anisotropy, associated with the features of polycrystalline structure of uterine wall histological sections with second-degree and third-degree endometriosis are determined

Diffuse tomography of optical anisotropy of tumors and necrotic conditions of biological tissues

An optical-physical system for extracting information about the fluctuations in the optical anisotropy of strongly scattering biological tissues is considered. A model is proposed for the formation of a depolarized background by birefringent and dichroic structures. An explicit form and symmetry of the completely depolarized component of the Mueller matrix is determined, a second-order differential matrix. An algorithm for the analytic determination of the distributions of elements of a second-order differential matrix is found. Interrelations between the magnitude of the elements of the second-order differential matrix and the fluctuations of the linear and circular birefringence-dichroism are obtained. The technique of diffuse tomography of an optically anisotropic component of strongly scattering biological tissues has been developed and experimentally tested. Maps of the distributions of the elements of the completely depolarized component of the Mueller matrix of the histological sections of the internal organs of the healthy and of the diabetic rats . The sensitivity, specificity and balanced accuracy of the method of diffuse tomography of the polycrystalline structure of tumors of the uterine wall and the degree of hemorrhage of the liver are determined

System 3D Jones-matrix polarimetry of polycrystalline films of biological fluids

A model of multilayer polycrystalline structure of films of biological fluids of human organs has been developed. Each layer is associated with a partial Jones-matrix operator of phase and amplitude anisotropy. A new principle for detecting polarization-inhomogeneous object fields using a coherent laser wave is proposed. Algorithms for digital holographic reconstruction of field distributions of complex amplitudes in the plane of a polycrystalline film of a biological fluid are found. A new optical technique is proposed: direct measurement of 3D distributions of elements of the Jones matrix. Maps of layer-by-layer distributions of elements of the Jones matrix of polycrystalline urine films are tudied. Sensitivity, specificity and balanced accuracy of the 3D Jones-matrix tomography method of the polycrystalline structure of urine films of healthy donors and patients with lbuminuria were determined. Within the framework of the statistical analysis of stratified maps of elements of the Jones matrix of polycrystalline urine films, objective criteria for the early diagnosis of the onset and course of albuminuria were found

Jones matrix polarization-correlation mapping of biological crystals networks

It has been proposed the optical model of Jones-matrix description of mechanisms of optical anisotropy of polycrystalline films of human bile, namely optical activity and birefringence. The algorithm of reconstruction of distributions of parameters - optical rotation angles and phase shifts of the indicated anisotropy types has been elaborated. The objective criteria of differentiation of bile films taken from healthy donors and patients with cholelithiasis by means of statistic analysis of such distributions have been determined. The operational characteristics (sensitivity, specificity and accuracy) of Jones-matrix reconstruction method of optical anisotropy parameters were defined