43 research outputs found
Nutation Sequences for Magnetic Resonance Imaging in Solids
Novel radio-frequency NMR pulse sequences are presented and their application to imaging of solids with use of rf field gradients is discussed. The sequences cause a nuclear spin to precess about the static field direction at a rate proportional to the strength of certain of the pulses. This forced precession is independent of the resonance offset and of couplings to other spins. The pulse-sequence design is described by means of coherent averaging theory and is confirmed experimentally and numerically
New approaches to ultrasensitive magnetic resonance
Spectroscopic methods tend to exhibit an inverse correlation between sensitivity and the ability to discriminate between similar structures. Were they obtainable with adequate sensitivity, magnetic resonance spectra could resolve structural controversies involving the nature of clusters, ions, semiconductor defects and catalytic intermediates. This paper describes several novel approaches to magnetic resonance, which have in common that the spins are coupled to other degrees of freedom in order to obtain nonequilibrium polarization and/or greater detection sensitivity. The methods under development include single-ion electron spin resonance (ESR) detected by ion trapping frequencies, catalyst NMR detected by the branching ratio to different spin symmetry species, and semiconductor nuclear magnetic resonance (NMR) detected via the circular polarization of luminescence
Optical Larmor Beat Detection of High-Resolution Nuclear Magnetic Resonance in a Semiconductor Heterostructure
A new method of optical nuclear magnetic resonance, Larmor beat detection, is described and demonstrated on a III-V semiconductor heterostructure. Modulation of the circular polarization of luminescence at the difference between two nuclear spin precession frequencies is induced by rf pulses. One isotope provides a spin-locked reference field, while NMR transients of a second isotope are observed optically in real time. Order-of-magnitude improvement in resolution and sensitivity over previous techniques is obtained, revealing weak electric field gradients in single epitaxial structures
New approaches to ultrasensitive magnetic resonance
Spectroscopic methods tend to exhibit an inverse correlation between sensitivity and the ability to discriminate between similar structures. Were they obtainable with adequate sensitivity, magnetic resonance spectra could resolve structural controversies involving the nature of clusters, ions, semiconductor defects and catalytic intermediates. This paper describes several novel approaches to magnetic resonance, which have in common that the spins are coupled to other degrees of freedom in order to obtain nonequilibrium polarization and/or greater detection sensitivity. The methods under development include single-ion electron spin resonance (ESR) detected by ion trapping frequencies, catalyst NMR detected by the branching ratio to different spin symmetry species, and semiconductor nuclear magnetic resonance (NMR) detected via the circular polarization of luminescence
Multiple-Quantum Spin Dynamics of Entanglement
Dynamics of entanglement is investigated on the basis of exactly solvable
models of multiple-quantum (MQ) NMR spin dynamics. It is shown that the time
evolution of MQ coherences of systems of coupled nuclear spins in solids is
directly connected with dynamics of the quantum entanglement. We studied
analytically dynamics of entangled states for two- and three-spin systems
coupled by the dipole-dipole interaction. In this case dynamics of the quantum
entanglement is uniquely determined by the time evolution of MQ coherences of
the second order. The real part of the density matrix describing MQ dynamics in
solids is responsible for MQ coherences of the zeroth order while its imaginary
part is responsible for the second order. Thus, one can conclude that dynamics
of the entanglement is connected with transitions from the real part of the
density matrix to the imaginary one and vice versa. A pure state which
generalizes the GHZ and W states is found. Different measures of the
entanglement of this state are analyzed for three-partite systems.Comment: 11 pages, 4 figure
Optical Larmor Beat Detection of High-Resolution Nuclear Magnetic Resonance in a Semiconductor Heterostructure
A Non-Coding RNA Within the Rasgrf1 Locus in Mouse Is Imprinted and Regulated by Its Homologous Chromosome in Trans
BACKGROUND: Rasgrf1 is imprinted in mouse, displaying paternal allele specific expression in neonatal brain. Paternal expression is accompanied by paternal-specific DNA methylation at a differentially methylated domain (DMD) within the locus. The cis-acting elements necessary for Rasgrf1 imprinting are known. A series of tandem DNA repeats control methylation of the adjacent DMD, which is a methylation sensitive enhancer-blocking element. These two sequences constitute a binary switch that controls imprinting and represents the Imprinting Control Region (ICR). One paternally transmitted mutation, which helped define the ICR, induced paramutation, in trans, on the maternal allele. Like many imprinted genes, Rasgrf1 lies within an imprinted cluster. One of four noncoding transcripts in the cluster, AK015891, is known to be imprinted. METHODOLOGY/PRINCIPAL FINDINGS: Here we demonstrate that an additional noncoding RNA, AK029869, is imprinted and paternally expressed in brain throughout development. Intriguingly, any of several maternally inherited ICR mutations affected expression of the paternal AK029869 transcript in trans. Furthermore, we found that the ICR mutations exert different trans effects on AK029869 at different developmental times. CONCLUSIONS/SIGNIFICANCE: Few trans effects have been defined in mammals and, those that exist, do not show the great variation seen at the Rasgrf1 imprinted domain, either in terms of the large number of mutations that produce the effects or the range of phenotypes that emerge when they are seen. These results suggest that trans regulation of gene expression may be more common than originally appreciated and that where trans regulation occurs it can change dynamically during development
Identification of Trypanosoma brucei RMI1/BLAP75 Homologue and Its Roles in Antigenic Variation
At any time, each cell of the protozoan parasite Trypanosoma brucei expresses a single species of its major antigenic protein, the variant surface glycoprotein (VSG), from a repertoire of >2,000 VSG genes and pseudogenes. The potential to express different VSGs by transcription and recombination allows the parasite to escape the antibody-mediated host immune response, a mechanism known as antigenic variation. The active VSG is transcribed from a sub-telomeric polycistronic unit called the expression site (ES), whose promoter is 40–60 kb upstream of the VSG. While the mechanisms that initiate recombination remain unclear, the resolution phase of these reactions results in the recombinational replacement of the expressed VSG with a donor from one of three distinct chromosomal locations; sub-telomeric loci on the 11 essential chromosomes, on minichromosomes, or at telomere-distal loci. Depending on the type of recombinational replacement (single or double crossover, duplicative gene conversion, etc), several DNA-repair pathways have been thought to play a role. Here we show that VSG recombination relies on at least two distinct DNA-repair pathways, one of which requires RMI1-TOPO3α to suppress recombination and one that is dependent on RAD51 and RMI1. These genetic interactions suggest that both RAD51-dependent and RAD51-independent recombination pathways operate in antigenic switching and that trypanosomes differentially utilize recombination factors for VSG switching, depending on currently unknown parameters within the ES
Expression of Odorant Receptor Family, Type 2 OR in the Aquatic Olfactory Cavity of Amphibian Frog Xenopus tropicalis
Recent genome wide in silico analyses discovered a new family (type 2 or family H) of odorant receptors (ORs) in teleost fish and frogs. However, since there is no evidence of the expression of these novel OR genes in olfactory sensory neurons (OSN), it remains unknown if type 2 ORs (OR2) function as odorant receptors. In this study, we examined expression of OR2 genes in the frog Xenopus tropicalis. The overall gene expression pattern is highly complex and differs depending on the gene and developmental stage. RT-PCR analysis in larvae showed that all of the OR2η genes we identified were expressed in the peripheral olfactory system and some were detected in the brain and skin. Whole mount in situ hybridization of the larval olfactory cavity confirmed that at least two OR2η genes so far tested are expressed in the OSN. Because tadpoles are aquatic animals, OR2η genes are probably involved in aquatic olfaction. In adults, OR2η genes are expressed in the nose, brain, and testes to different degrees depending on the genes. OR2η expression in the olfactory system is restricted to the medium cavity, which participates in the detection of water-soluble odorants, suggesting that OR2ηs function as receptors for water-soluble odorants. Moreover, the fact that several OR2ηs are significantly expressed in non-olfactory organs suggests unknown roles in a range of biological processes other than putative odorant receptor functions