9 research outputs found
Biofouling Removal and Protein Detection Using a Hypersonic Resonator
Nonspecific binding
(NSB) is a general issue for surface based
biosensors. Various approaches have been developed to prevent or remove
the NSBs. However, these approaches either increased the background
signals of the sensors or limited to specific transducers interface.
In this work, we developed a hydrodynamic approach to selectively
remove the NSBs using a microfabricated hypersonic resonator with
2.5 gigahertz (GHz) resonant frequency. The high frequency device
facilitates generation of multiple controlled microvortexes which
then create cleaning forces at the solid–liquid interfaces.
The competitive adhesive and cleaning forces have been investigated
using the finite element method (FEM) simulation, identifying the
feasibility of the vortex-induced NSB removal. NSB proteins have been
selectively removed experimentally both on the surface of the resonator
and on other substrates which contact the vortexes. Thus, the developed
hydrodynamic approach is believed to be a simple and versatile tool
for NSB removal and compatible to many sensor systems. The unique
feature of the hypersonic resonator is that it can be used as a gravimetric
sensor as well; thus a combined NSB removal and protein detection
dual functional biosensor system is developed
Knowledge and Attitudes as Influencing Factors For Adopting Health Care Technology Among Medical Students in Germany
Dockweiler C, Hornberg C. Knowledge and Attitudes as Influencing Factors For Adopting Health Care Technology Among Medical Students in Germany. JOURNAL OF THE INTERNATIONAL SOCIETY FOR TELEMEDICINE AND EHEALTH. 2014;2(1):64-70
Presence of <i>Toxoplasma gondii</i> in tissue samples from five different organs in 16 macropod marsupials of three species (<i>Macropus rufus, M. fuliginosus and M. robustus</i>) tested by direct PCR.
<p>Presence of <i>Toxoplasma gondii</i> in tissue samples from five different organs in 16 macropod marsupials of three species (<i>Macropus rufus, M. fuliginosus and M. robustus</i>) tested by direct PCR.</p
Multi-locus genotypes of <i>Toxoplasma gondii</i> by direct PCR and sequencing of tissue samples from macropods.
<p>U indicates non-archetypal allele. I, II and III refer to archetypal alleles from type I, II and III strains. NT indicates that the sample was not amplified.</p
A Universal Biomolecular Concentrator To Enhance Biomolecular Surface Binding Based on Acoustic NEMS Resonator
In designing bioassay systems for
low-abundance biomolecule detection,
most research focuses on improving transduction mechanisms while ignoring
the intrinsically fundamental limitations in solution: mass transfer
and binding affinity. We demonstrate enhanced biomolecular surface
binding using an acoustic nano-electromechanical system (NEMS) resonator,
as an on-chip biomolecular concentrator which breaks both mass transfer
and binding affinity limitations. As a result, a concentration factor
of 10<sup>5</sup> has been obtained for various biomolecules. The
resultantly enhanced surface binding between probes on the absorption
surface and analytes in solution enables us to lower the limit of
detection for representative proteins. We also integrated the biomolecular
concentrator into an optoelectronic bioassay platform to demonstrate
delivery of proteins from buffer/serum to the absorption surface.
Since the manufacture of the resonator is CMOS-compatible, we expect
it to be readily applied to further analysis of biomolecular interactions
in molecular diagnostics
A Universal Biomolecular Concentrator To Enhance Biomolecular Surface Binding Based on Acoustic NEMS Resonator
In designing bioassay systems for
low-abundance biomolecule detection,
most research focuses on improving transduction mechanisms while ignoring
the intrinsically fundamental limitations in solution: mass transfer
and binding affinity. We demonstrate enhanced biomolecular surface
binding using an acoustic nano-electromechanical system (NEMS) resonator,
as an on-chip biomolecular concentrator which breaks both mass transfer
and binding affinity limitations. As a result, a concentration factor
of 10<sup>5</sup> has been obtained for various biomolecules. The
resultantly enhanced surface binding between probes on the absorption
surface and analytes in solution enables us to lower the limit of
detection for representative proteins. We also integrated the biomolecular
concentrator into an optoelectronic bioassay platform to demonstrate
delivery of proteins from buffer/serum to the absorption surface.
Since the manufacture of the resonator is CMOS-compatible, we expect
it to be readily applied to further analysis of biomolecular interactions
in molecular diagnostics
A Universal Biomolecular Concentrator To Enhance Biomolecular Surface Binding Based on Acoustic NEMS Resonator
In designing bioassay systems for
low-abundance biomolecule detection,
most research focuses on improving transduction mechanisms while ignoring
the intrinsically fundamental limitations in solution: mass transfer
and binding affinity. We demonstrate enhanced biomolecular surface
binding using an acoustic nano-electromechanical system (NEMS) resonator,
as an on-chip biomolecular concentrator which breaks both mass transfer
and binding affinity limitations. As a result, a concentration factor
of 10<sup>5</sup> has been obtained for various biomolecules. The
resultantly enhanced surface binding between probes on the absorption
surface and analytes in solution enables us to lower the limit of
detection for representative proteins. We also integrated the biomolecular
concentrator into an optoelectronic bioassay platform to demonstrate
delivery of proteins from buffer/serum to the absorption surface.
Since the manufacture of the resonator is CMOS-compatible, we expect
it to be readily applied to further analysis of biomolecular interactions
in molecular diagnostics
A Universal Biomolecular Concentrator To Enhance Biomolecular Surface Binding Based on Acoustic NEMS Resonator
In designing bioassay systems for
low-abundance biomolecule detection,
most research focuses on improving transduction mechanisms while ignoring
the intrinsically fundamental limitations in solution: mass transfer
and binding affinity. We demonstrate enhanced biomolecular surface
binding using an acoustic nano-electromechanical system (NEMS) resonator,
as an on-chip biomolecular concentrator which breaks both mass transfer
and binding affinity limitations. As a result, a concentration factor
of 10<sup>5</sup> has been obtained for various biomolecules. The
resultantly enhanced surface binding between probes on the absorption
surface and analytes in solution enables us to lower the limit of
detection for representative proteins. We also integrated the biomolecular
concentrator into an optoelectronic bioassay platform to demonstrate
delivery of proteins from buffer/serum to the absorption surface.
Since the manufacture of the resonator is CMOS-compatible, we expect
it to be readily applied to further analysis of biomolecular interactions
in molecular diagnostics
A Universal Biomolecular Concentrator To Enhance Biomolecular Surface Binding Based on Acoustic NEMS Resonator
In designing bioassay systems for
low-abundance biomolecule detection,
most research focuses on improving transduction mechanisms while ignoring
the intrinsically fundamental limitations in solution: mass transfer
and binding affinity. We demonstrate enhanced biomolecular surface
binding using an acoustic nano-electromechanical system (NEMS) resonator,
as an on-chip biomolecular concentrator which breaks both mass transfer
and binding affinity limitations. As a result, a concentration factor
of 10<sup>5</sup> has been obtained for various biomolecules. The
resultantly enhanced surface binding between probes on the absorption
surface and analytes in solution enables us to lower the limit of
detection for representative proteins. We also integrated the biomolecular
concentrator into an optoelectronic bioassay platform to demonstrate
delivery of proteins from buffer/serum to the absorption surface.
Since the manufacture of the resonator is CMOS-compatible, we expect
it to be readily applied to further analysis of biomolecular interactions
in molecular diagnostics