Simultaneous Monitoring of Multiple People's Vital Sign Leveraging a Single Phased-MIMO Radar

Vital sign monitoring plays a critical role in tracking the physiological state of people and enabling various health-related applications (e.g., recommending a change of lifestyle, examining the risk of diseases). Traditional approaches rely on hospitalization or body-attached instruments, which are costly and intrusive. Therefore, researchers have been exploring contact-less vital sign monitoring with radio frequency signals in recent years. Early studies with continuous wave radars/WiFi devices work on detecting vital signs of a single individual, but it still remains challenging to simultaneously monitor vital signs of multiple subjects, especially those who locate in proximity. In this paper, we design and implement a time-division multiplexing (TDM) phased-MIMO radar sensing scheme for high-precision vital sign monitoring of multiple people. Our phased-MIMO radar can steer the mmWave beam towards different directions with a micro-second delay, which enables capturing the vital signs of multiple individuals at the same radial distance to the radar. Furthermore, we develop a TDM-MIMO technique to fully utilize all transmitting antenna (TX)-receiving antenna (RX) pairs, thereby significantly boosting the signal-to-noise ratio. Based on the designed TDM phased-MIMO radar, we develop a system to automatically localize multiple human subjects and estimate their vital signs. Extensive evaluations show that under two-subject scenarios, our system can achieve an error of less than 1 beat per minute (BPM) and 3 BPM for breathing rate (BR) and heartbeat rate (HR) estimations, respectively, at a subject-to-radar distance of $1.6~m$. The minimal subject-to-subject angle separation is $40{\deg}$, corresponding to a close distance of $0.5~m$ between two subjects, which outperforms the state-of-the-art

Experimental and Numerical Research on Seismic Performance of Earthquake-Damaged RC Frame Strengthened with CFRP Sheets

In order to assess the seismic performance of damaged reinforced concrete (RC) frame strengthened with carbon fiber reinforced polymers (CFRP) sheets, two experimental specimens with identical reinforcement ratio and geometric dimensions were designed following Chinese code for seismic design of buildings. Experimental specimens consist of a reference (undamaged) RC frame, namely, KJ-1, and an earthquake-damaged RC frame strengthened with CFRP sheets, namely, KJ-2. A pseudostatic test was conducted on the two specimens to simulate moderate earthquake damage. The strengthening effects of CFRP sheets on damaged RC frame were discussed in terms of hysteretic curve, skeleton curve, stiffness degradation, and ductility. In addition, numerical method based on fiber model method was utilized to analyze the seismic performance of KJ-1 and KJ-2 and it is compared with the experimental result. Both the results confirm that the method of exterior bonding CFRP sheets on the damaged RC frame has restored the seismic performance such as bearing capacity, stiffness, and ductility to its original undamaged level, and some of the seismic performance of the damaged RC frame strengthened with CFRP sheets is even better than the undamaged one, which proves that the method has significant effect in strengthening postearthquake-damaged RC frames

Transcriptional regulation of BRD7 expression by Sp1 and c-Myc

<p>Abstract</p> <p>Background</p> <p>Bromodomain is an evolutionally conserved domain that is found in proteins strongly implicated in signal-dependent transcriptional regulation. Genetic alterations of bromodomain genes contributed to the development of many human cancers and other disorders. BRD7 is a recently identified bromodomain gene. It plays a critical role in cellular growth, cell cycle progression, and signal-dependent gene expression. Previous studies showed that BRD7 gene exhibited much higher-level of mRNA expression in normal nasopharyngeal epithelia than in nasopharyngeal carcinoma (NPC) biopsies and cell lines. However, little is known about its transcriptional regulation. In this study, we explored the transcriptional regulation of BRD7 gene.</p> <p>Method</p> <p>Potential binding sites of transcription factors within the promoter region of BRD7 gene were predicted with MatInspector Professional <url>http://genomatix.de/cgi-bin/matinspector_prof/mat_fam.pl</url>. Mutation construct methods and luciferase assays were performed to define the minimal promoter of BRD7 gene. RT-PCR and western blot assays were used to detect the endogenous expression of transcription factor Sp1, c-Myc and E2F6 in all cell lines used in this study. Electrophoretic mobility shift assays (EMSA) and Chromatin immunoprecipitation (ChIP) were used to detect the direct transcription factors that are responsible for the promoter activity of BRD7 gene. DNA vector-based siRNA technology and cell transfection methods were employed to establish clone pools that stably expresses SiRNA against c-Myc expression in nasopharyngeal carcinoma 5-8F cells. Real-time PCR was used to detect mRNA expression of BRD7 gene in 5-8F/Si-c-Myc cells.</p> <p>Results</p> <p>We defined the minimal promoter of BRD7 gene in a 55-bp region (from -266 to -212bp), and identified that its promoter activity is inversely related to c-Myc expression. Sp1 binds to the Sp1/Myc-Max overlapping site of BRD7 minimal promoter, and slightly positively regulate its promoter activity. c-Myc binds to this Sp1/Myc-Max overlapping site as well, and negatively regulates the promoter activity and endogenous mRNA expression of BRD7 gene. Knock-down of c-Myc increases the promoter activity and mRNA level of BRD7 gene. The luciferase activity of the mutated promoter constructs showed that Sp1/Myc-Max overlapping site is a positive regulation element of BRD7 promoter.</p> <p>Conclusion</p> <p>These studies provide for the first time the evidence that c-Myc is indeed a negative regulator of BRD7 gene. These findings will help to further understand and uncover the bio-functions of BRD7 gene involved in the pathogenesis of NPC.</p

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Experimental Analysis and Discussion on the Damage Variable of Frozen Loess

The damage variable is very important to study damage evolution of material. Taking frozen loess as an example, a series of triaxial compression and triaxial loading-unloading tests are performed under five strain rates of 5.0 × 10−6–1.3 × 10−2/s at a temperature of −6°C. A damage criterion of frozen loess is defined and a damage factor Dc is introduced to satisfy the requirements of the engineering application. The damage variable of frozen loess is investigated using the following four methods: the stiffness degradation method, the deformation increase method, the dissipated energy increase method, and the constitutive model deducing method during deformation process. In addition, the advantages and disadvantages of the four methods are discussed when they are used for frozen loess material. According to the discussion, the plastic strain may be the most appropriate variable to characterize the damage evolution of frozen loess during the deformation process based on the material properties and the nature of the material service

High-strength lightweight blocks prepared from the by-product of aluminium removed from fly ash

A large amount of powder by-product is generated when Al2O3 is extracted from fly ash. The purpose of this study is to investigate the properties of the powder and find an effective method to reuse it. XRF, XRD, and SEM were used to analysis its compositions and microstructures. The powder was then compressed at the pressure of from 20 to 100 MPa. Results show that the powder was disordered calcium silicate hydrate with an average Ca/Si molar ratio of 1.08. It becomes hardened blocks by compressing. The bulk density of the hardened blocks is between 500 and 800 kg m-3 corresponding to the pressure of 20 to 60 MPa. Their flexible strength is from 1.60 to 5.35 MPa, and compressive strength from 8.50 to 30.50 MPa. The blocks can be recycled by repeating the procedure of crushing blocks, grinding them into powder, and compressing the powder into hardened blocks. This powder can be sustainably reused to prepare lightweight blocks with high strength

Influence of Hydrothermal Synthesis Conditions on the Formation of Calcium Silicate Hydrates: from Amorphous to Crystalline Phases

Hydrothermal treatment has been widely applied in the synthesis of well crystalline calcium silicate hydrate (CSH), such as tobermorite and xonotlite. However, both morphology and crystallinity of CSH are greatly affected by the conditions of hydrothermal treatment including siliceous materials, temperature increase rate and isothermal periods. In this study, the influence of hydrothermal conditions on the growth of nano-crystalline CSH was investigated based on XRD analysis. Results showed that siliceous materials with amorphous nature (i e, nano silica powder) are beneficial to synthesize pure amorphous CSH, while the use of more crystallized siliceous materials (i e, diatomite and quartz powder) leads to producing crystalline CSH. Results also indicate that the formation of tobermorite and xonotlite is greatly affected by the temperature rise rate during hydrothermal treatment