Metabolic profile, bioavailability and toxicokinetics of zearalenone-14-glucoside in rats after oral and intravenous administration by liquid chromatography high-resolution mass spectrometry and tandem mass spectrometry

Zearalenone-14-glucoside (ZEN-14G), a key modified mycotoxin, has attracted a great deal of attention due to the possible conversion to its free form of zearalenone (ZEN) exerting toxicity. In this study, the toxicokinetics of ZEN-14G were investigated in rats after oral and intravenous administration. The plasma concentrations of ZEN-14G and its major five metabolites were quantified using a validated liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The data were analyzed via non-compartmental analysis using software WinNonlin 6.3. The results indicated that ZEN-14G was rapidly hydrolyzed into ZEN in vivo. In addition, the major parameters of ZEN-14G following intravenous administration were: area under the plasma concentration-time curve (AUC), 1.80 h.ng/mL; the apparent volume of distribution (V-Z), 7.25 L/kg; and total body clearance (CL), 5.02 mL/h/kg, respectively. After oral administration, the typical parameters were: AUC, 0.16 h.ng/mL; V-Z, 6.24 mL/kg; and CL, 4.50 mL/h/kg, respectively. The absolute oral bioavailability of ZEN-14G in rats was about 9%, since low levels of ZEN-14G were detected in plasma, which might be attributed to its extensive metabolism. Therefore, liquid chromatography high-resolution mass spectrometry (LC-HRMS) was adopted to clarify the metabolic profile of ZEN-14G in rats' plasma. As a result, eight metabolites were identified in which ZEN-14-glucuronic acid (ZEN-14GlcA) had a large yield from the first time-point and continued accumulating after oral administration, indicating that ZEN-14-glucuronic acid could serve a potential biomarker of ZEN-14G. The obtained outcomes would prompt the accurate safety evaluation of ZEN-14G

Large Model Based Referring Camouflaged Object Detection

Referring camouflaged object detection (Ref-COD) is a recently-proposed problem aiming to segment out specified camouflaged objects matched with a textual or visual reference. This task involves two major challenges: the COD domain-specific perception and multimodal reference-image alignment. Our motivation is to make full use of the semantic intelligence and intrinsic knowledge of recent Multimodal Large Language Models (MLLMs) to decompose this complex task in a human-like way. As language is highly condensed and inductive, linguistic expression is the main media of human knowledge learning, and the transmission of knowledge information follows a multi-level progression from simplicity to complexity. In this paper, we propose a large-model-based Multi-Level Knowledge-Guided multimodal method for Ref-COD termed MLKG, where multi-level knowledge descriptions from MLLM are organized to guide the large vision model of segmentation to perceive the camouflage-targets and camouflage-scene progressively and meanwhile deeply align the textual references with camouflaged photos. To our knowledge, our contributions mainly include: (1) This is the first time that the MLLM knowledge is studied for Ref-COD and COD. (2) We, for the first time, propose decomposing Ref-COD into two main perspectives of perceiving the target and scene by integrating MLLM knowledge, and contribute a multi-level knowledge-guided method. (3) Our method achieves the state-of-the-art on the Ref-COD benchmark outperforming numerous strong competitors. Moreover, thanks to the injected rich knowledge, it demonstrates zero-shot generalization ability on uni-modal COD datasets. We will release our code soon

Evaluation of the efficiency of Chinese energy-saving household appliance subsidy policy: an economic benefit perspective

China has made great efforts to control energy consumption and reduce environmental pressure in recent decades. In the residential sector, the dramatic increase in the ownership of household appliances has driven the growth of electricity consumption, which calls for effective energy-saving policies. In this study, we exemplified the sales of refrigerators in Beijing, aiming to evaluate the effectiveness of current subsidy policies for stimulating the purchase of energy-efficient household appliances. In specific, we first selected ten pairs of refrigerators from six brands having similar functions, however different in their energy efficiency grades (EEG). By applying a combination of net present value (NPV) difference method and Conversion Method of Electrical Engineering Coefficient, we calculated and compared the changes in the NPV difference and dynamic investment payback period (DIPP) to evaluate the effectiveness of the policy. The results revealed that economic benefits were mainly generated in EEG2; thereby we suggest that the government can cancel subsidies for EEG2 refrigerators, and increase the subsidy amount for EGG1 refrigerators at a potential rate of 24% in the future

On-chip single-mode thin film lithium niobate laser based on Sagnac loop reflectors

We demonstrate an on-chip single-mode Er3+-doped thin film lithium niobate (Er: TFLN) laser which consists of a Fabry-P\'erot (FP) resonator based on Sagnac loop reflectors (SLRs). The fabricated Er: TFLN laser has a footprint of 6.5 mmx1.5 mm with a loaded quality (Q) factor of 1.6x105 and a free spectral range (FSR) of 63 pm. We generate the single-mode laser around 1550-nm wavelength with a maximum output power of 44.7 {\mu}W and a slope efficiency of 0.18 %.Comment: 4 pages, 4 figure

Integrated electro-optically tunable narrow-linewidth III-V laser

We demonstrate an integrated electro-optically tunable narrow-linewidth III-V laser with an output power of 738.8 {\mu}W and an intrinsic linewidth of 45.55 kHz at the C band. The laser cavity is constructed using a fiber Bragg grating (FBG) and a tunable Sagnac loop reflector (TSLR) fabricated on thin film lithium niobate (TFLN). The combination of the FBG and the electro-optically tunable TSLR offers the advantages of single spatial mode, single-frequency, narrow-linewidth, and wide wavelength tunability for the electrically pumped hybrid integrated laser, which features a frequency tuning range of 20 GHz and a tuning efficiency of 0.8 GHz/V

Occurrence of neonicotinoids in Chinese apiculture and a corresponding risk exposure assessment

Neonicotinoids are the most widely used insecticides worldwide, but there is mounting evidence demonstrating that they have adverse effects on nontarget organisms. However, little is known about the extent of environmental neonicotinoids contamination in China. In this study, a total of 693 honey samples from across China, from both Apis melifera and Apis cerana, were analyzed to examine neonicotinoid concentrations and their geographical distribution, and correlation with the primary plant species from which the honey was obtained. Furthermore, chronic and acute exposure risk and risk ranking for humans eating honey were investigated, and risks to bees were also considered. The results revealed that 40.8% of the samples contained at least one of the five neonicotinoids tested. Honeys from commercial crops were found to be more frequently contaminated with neonicotinoids than those from noncommercial crops. Honey samples from Apis mellifera were more frequently contaminated than those from Apis cerana. The concentrations of neonicotinoids found in honey overlapped with those that have been found to have significant adverse effects on honeybee health. The dietary risk assessments indicated that the levels of neonicotinoids detected in honey were likely to be safe for human consumption

Uncoupling DISC1 × D2R Protein-Protein Interactions Facilitates Latent Inhibition in Disc1-L100P Animal Model of Schizophrenia and Enhances Synaptic Plasticity via D2 Receptors

Both Disrupted-In-Schizophrenia-1 (DISC1) and dopamine receptors D2R have significant contributions to the pathogenesis of schizophrenia. Our previous study demonstrated that DISC1 binds to D2R and such protein-protein interaction is enhanced in patients with schizophrenia and Disc1-L100P mouse model of schizophrenia (Su et al., 2014). By uncoupling DISC1 × D2R interaction (trans-activator of transcription (TAT)-D2pep), the synthesized TAT-peptide elicited antipsychotic-like effects in pharmacological and genetic animal models, without motor side effects as tardive dyskinesia commonly seen with typical antipsychotic drugs (APDs), indicating that the potential of TAT-D2pep of becoming a new APD. Therefore, in the current study, we further explored the APD-associated capacities of TAT-D2pep. We found that TAT-D2pep corrected the disrupted latent inhibition (LI), as a hallmark of schizophrenia associated endophenotype, in Disc1-L100P mutant mice—a genetic model of schizophrenia, supporting further APD’ capacity of TAT-D2pep. Moreover, we found that TAT-D2pep elicited nootropic effects in C57BL/6NCrl inbred mice, suggesting that TAT-D2pep acts as a cognitive enhancer, a desirable feature of APDs of the new generation. Namely, TAT-D2pep improved working memory in T-maze, and cognitive flexibility assessed by the LI paradigm, in C57BL/6N mice. Next, we assessed the impact of TAT-D2pep on hippocampal long-term plasticity (LTP) under basal conditions and upon stimulation of D2 receptors using quinpirole. We found comparable effects of TAT-D2pep and its control TAT-D2pep-scrambled peptide (TAT-D2pep-sc) under basal conditions. However, under stimulation of D2R by quinpirole, LTP was enhanced in hippocampal slices incubated with TAT-D2pep, supporting the notion that TAT-D2pep acts in a dopamine-dependent manner and acts as synaptic enhancer. Overall, our experiments demonstrated implication of DISC1 × D2R protein-protein interactions into mechanisms of cognitive and synaptic plasticity, which help to further understand molecular-cellular mechanisms of APD of the next generation

Asymmetric Total Synthesis of Arcutinidine and Arcutinine

We have accomplished the asymmetric total synthesis of arcutinidine and arcutinine, two arcutine-type C20-diterpenoid alkaloids. A pentacyclic intermediate was rapidly assembled by using two Diels−Alder reactions. A cascade sequence of Prins cyclization and Wagner−Meerwein rearrangement was then developed to construct the core of arcutinidine, which was further elaborated into an oxygenated pentacycle through a scalable route. Chemoselective reductive amination followed by spontaneous imine formation furnished the pyrroline motif at a final stage

Risk Factors for Necrotizing Enterocolitis in Neonates: A Retrospective Case-Control Study

Necrotizing enterocolitis (NEC) in neonates is devastating, and risk-factor identification is crucial. This study aimed to evaluate risk factors for NEC in different gestational age (GA) groups. Methods: Risk factors associated with NEC were investigated using a retrospective case-control design. Patients with Bell's Stage NEC ≥ II were divided into three groups based on GA: I, <34 weeks; II, ≥34 weeks but <37 weeks; III, ≥37 weeks. Each case was paired with two GA- and weight-matched controls. Data were collected from medical records, and univariate and conditional logistic regression analyses employed. Results: A total of 238 cases and 476 controls were enrolled. Variation in the months when NEC was diagnosed was noted, with a peak in January and a trough in August. Intrahepatic cholestasis of pregnancy and transfusion with packed red blood cells were significantly associated with NEC in preterm infants. Meconium aspiration syndrome was an independent risk factor for a greater chance of NEC development in full-term infants. Postnatal asphyxia and sepsis were associated with an increased risk of NEC in all groups. Probiotic use was associated with a reduced risk of NEC. Patients aged >34 weeks with congenital heart disease were more likely than controls to have NEC. Conclusion: Intrahepatic cholestasis of pregnancy and meconium aspiration syndrome may be new risk factors for NEC

Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China

Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family Solemoviridae. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”