Dragon, 1942

https://red.mnstate.edu/yearbooks/1026/thumbnail.jp

Table_6_Involvement of JMJ15 in the dynamic change of genome-wide H3K4me3 in response to salt stress.XLSX

Post-translational histone modifications play important roles in regulating chromatin structure and transcriptional regulation. Histone 3 lysine 4 trimethylation (H3K4me3) is a prominent histone modification mainly associated with gene activation. Here we showed that a histone demethylase, JMJ15, belonging to KDM5/JARID group, is involved in salt stress response in Arabidopsis thaliana. Jmj15 loss-of-function mutants displayed increased sensitivity to salt stress. Moreover, knockout of JMJ15 impaired the salt responsive gene expression program and affected H3K4me3 levels of many stress-related genes under salt-stressed condition. Importantly, we demonstrated that JMJ15 regulated the expression level of two WRKY transcription factors, WRKY46 and WRKY70, which were negatively involved in abiotic stress tolerance. Furthermore, JMJ15 directly bound to and demethylated H3K4me3 mark in the promoter and coding regions of WRKY46 and WRKY70, thereby repressing these two WRKY gene expression under salt stress. Overall, our study revealed a novel molecular function of the histone demethylase JMJ15 under salt stress in plants.</p

Selective Co(II) Adsorption Using Hollow ZIF‑8 Nanostructures with Embedded Fe₃O₄ Nanoparticles

In this study, we developed a magnetic hollow metal–organic framework (Fe3O4/HZIF-8) nanocomposite by modifying ZIF-8 nanocrystals with magnetic Fe3O4 nanoparticles at room temperature. The resulting Fe3O4/HZIF-8 nanostructured composite was used as an absorbent for Co(II) elimination. Due to the functionalization of Fe3O4 nanoparticles and the hollow structure of ZIF-8, the prepared absorbent showed a maximum adsorption capacity of 155.8 mg g–1 within 4 h and could be easily separated from the matrix using magnetization. Additionally, the absorbent exhibited a wide pH tolerance range from pH 2.0 to 9.0 and maintained excellent selectivity for Co(II) adsorption in simulated wastewater. The experimental data was well described by the pseudo-second-order kinetic model and the Langmuir adsorption isotherm model. The ultraviolet–visible spectroscopy (UV–vis), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) studies further indicated that complexation reactions dominated the adsorption of Co(II). Thereby, the nanostructured hollow MOF could be performed as a high-performance absorbent for pollutant removal. This work sheds light on the nanostructured design and uptake mechanism study of the metal ions and radionuclide removal

DataSheet_1_Real-world outcomes of immunotherapy-based neoadjuvant therapy in resectable non-small cell lung cancer.docx

ObjectivesRecent clinical studies have demonstrated that immunotherapy-based neoadjuvant therapy have promising effectiveness for patients with resectable non-small cell lung cancer (NSCLC) in terms of pathologic response. Therefore, we performed this study to investigate whether immunotherapy-based neoadjuvant therapy is effective and safe for patients with resectable NSCLC.Materials and methodsThis open-label observational two-arm clinical study was performed at Shanghai Chest Hospital in China with patients who had resectable NSCLC and received two to three cycles of immunotherapy-based neoadjuvant therapy or neoadjuvant chemotherapy alone, followed by surgical resection. The primary endpoint was a major pathologic response (MPR). The secondary endpoints include a complete pathological response (pCR), a radiologic response to neoadjuvant therapy (TRR), event-free survival (EFS), and overall survival (OS).ResultsA total of 51 patients was included in this clinical study, of which 31 patients received immunotherapy-based neoadjuvant therapy and 20 patients received neoadjuvant chemotherapy alone. The percentage of patients achieving a major pathologic response was 41.9% with immunotherapy-based neoadjuvant therapy and 15.0% (95% CI, 0.008 to 0.468; P = 0.043) with neoadjuvant chemotherapy alone. The percentage of patients with pathologic complete response was 19.4% in the immunotherapy-based group and 5% (95% CI, -0.069 to 0.318; P = 0.223) in the chemotherapy group. The radiographic response rate was 71% after immunotherapy-based neoadjuvant therapy and 60% (95% CI, -0.143 to 0.359; P = 0.417) after neoadjuvant chemotherapy. At a median follow-up of 28 months, the median EFS and OS endpoints were not reached.ConclusionsNeoadjuvant immunotherapy offers a considerable advantage over chemotherapy alone for resectable NSCLC in terms of the major pathologic response. Moreover, it did not enhance the risk of adverse events or hinder surgical resection.</p

Controlled Synthesis of Hierarchical Nanostructured Metal Ferrite Microspheres for Enhanced Electrocatalytic Oxygen Evolution Reaction

Ferrite MFe2O4 (M = Ni, Co, etc.) metal oxides have been the focus of intense research, studied as promising electrocatalysts due to their good catalytic activity and stability for oxygen evolution reaction (OER). Structure engineering is a significant solution to achieve a high catalytic performance; further, optimizing the structure and specific surface area (SSA) of metal ferrite (CoFe2O4 and NiFe2O4) is regarded as a good choice. Herein, we designed hierarchical porous nanostructured CoFe2O4 and NiFe2O4 microspheres with the assistance of sodium dodecyl sulfonate by solvothermal and annealing methods. We found that three-dimensional microspheres assembled by nanoparticles and porous nanosheets exhibit higher SSAs for NiFe2O4 (158.47 m2 g–1) and CoFe2O4 (144.52 m2 g–1). Their higher SSA is much higher than those of other CoFe2O4-based and NiFe2O4-based materials. The as-prepared metal ferrite microspheres exhibit excellent OER electrocatalytic activity with lower Tafel slopes (55.2 and 58.2 mV dec–1) and lower overpotentials (235 and 280 mV) at a constant current density of 10 mA cm–2 and long-term stability, which is better than that of NiFe2O4 and CoFe2O4 bulks. This method for preparing a large SSA is expected to be applied in other spinel metal oxides

Additional file 3: of Bexarotene inhibits the viability of non-small cell lung cancer cells via slc10a2/PPARγ/PTEN/mTOR signaling pathway

Figure S3. (A) The expression of apoptotic related genes Bcl-2, cyclin D1, c-FLIP, caspase 3, caspase 7 in H1299 cells when treated with bexarotene, bexarotene in combination with GW9662 respectively. (B) The expression of apoptotic related genes Bcl-2, cyclin D1, c-FLIP, caspase 3, caspase 7 in slc10a2 overexpressed H1299 cells when treated with bexarotene, bexarotene in combination with GW9662 respectively. (C) The expression of tumor suppressor genes PTEN, P21, P53, LKB1, TSC2 in H1299 cells when treated with bexarotene, bexarotene in combination with GW9662 respectively. (D) The expression of tumor suppressor genes PTEN, P21, P53, LKB1, TSC2 in slc10a2 overexpressed H1299 cells when treated with bexarotene, bexarotene in combination with GW9662 respectively. H1299 cells without any treatment as control group. All experiments were repeated 3 times. (TIFF 882 kb

Additional file 2: of Bexarotene inhibits the viability of non-small cell lung cancer cells via slc10a2/PPARγ/PTEN/mTOR signaling pathway

Figure S2. (A) The expression of apoptotic related genes Bcl-2, cyclin D1, c-FLIP, caspase 3, caspase 7 in H1299 cells treated with bexarotene, overexpressed slc10a2 in combination with bexarotene, slc10a2-shRNA in combination with bexarotene respectively. (B) The expression of tumor suppressor genes PTEN, P21, P53, LKB1, TSC2 in H1299 cells treated with bexarotene, overexpressed slc10a2 in combination with bexarotene, slc10a2-shRNA in combination with bexarotene respectively, H1299 cells without any treatment as control group. All experiments were repeated 3 times. (TIFF 516 kb

El Diario de Pontevedra : periódico liberal: Ano XLIII Número 12458 - 1926 xullo 29

AZD4547 induces autophagy. (A) H1581 and H520 cells grown in 24-well plates were treated with DMSO, or AZD4547 (1 μM) for 24 h. MDC staining was performed and the cells were examined under a fluorescence microscope. Scale bars represent 25 μm. (B) Quantitative results of MDC staining in conditions shown in (A); mean ± SD, n = 3, **p < 0.01. (TIF 1577 kb

Meta-analysis results of common SNPs of miRNAs.

<p>Results are shown as OR 95% CI; M1: homozygote comparison; M2 heterozygote comparison; M3: dominant model; M4: recessive model;</p>*<p>for significant difference.</p

Additional file 2: Table S2. of FGF2/FGFR1 regulates autophagy in FGFR1-amplified non-small cell lung cancer cells

Sequences of all siRNA constructs that were used in the study. (TIF 67 kb