A prelude report on molecular docking of HER2 protein towards comprehending anti-cancer properties of saponins from Solanum tuberosum

Saponins are extensively known for many biological activities e.g. antimicrobial, anti-palatability, anti-cancer and hemolytic. As cancer cells have a more cholesterol-like compound in their membrane structure the saponins bind cholesterol due to their natural affinity to bind cancer cell membrane. This prevents them from entering the body through the intestinal tract, where they have the ability to attach themselves to vital organs and grow. This study reports the effective use of lower dose saponins like immunotoxin so that they can inhibit the proliferation of cancerous pancreatic cells. The investigation of pancreatic cancer metabolic pathway it was found that proteins 3H3B produced by genes HER-2 are involved in the enhancement of this type of cancer. Further docking studies showed that there is an effective interaction between saponins and cancer cells. The glide score of the saponin analogue compound with CID 21573770 (Pubchem) was -6.30 followed by score of -6.05 and -5.29 for 5-Florouracil and gemcitabine respectively. The interaction was observed in the GLU and GLN rich region, saponins made H-bonds with GLU-188, GLN-119, VAL-72 and GLN-71. This study indicates an effective way towards leading newer prospects for developing saponin analogue based cancer-fighting drugs with improved cancer cell inhibition property without killing normal cells

Gene Editing and Systems Biology Tools for Pesticide Bioremediation: A Review

Bioremediation is the degradation potential of microorganisms to dissimilate the complex chemical compounds from the surrounding environment. The genetics and biochemistry of biodegradation processes in datasets opened the way of systems biology. Systemic biology aid the study of interacting parts involved in the system. The significant keys of system biology are biodegradation network, computational biology, and omics approaches. Biodegradation network consists of all the databases and datasets which aid in assisting the degradation and deterioration potential of microorganisms for bioremediation processes. This review deciphers the bio-degradation network, i.e., the databases and datasets (UM-BBD, PAN, PTID, etc.) aiding in assisting the degradation and deterioration potential of microorganisms for bioremediation processes, computational biology and multi omics approaches like metagenomics, genomics, transcriptomics, proteomics, and metabolomics for the efficient functional gene mining and their validation for bioremediation experiments. Besides, the present review also describes the gene editing tools like CRISPR Cas, TALEN, and ZFNs which can possibly make design microbe with functional gene of interest for degradation of particular recalcitrant for improved bioremediation

Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review

The protein productions strategies are crucial towards the development of application based research and elucidating the novel purification strategies for industrial production. Currently, there are few innovative avenues are studies for cloning, upstream, and purification through efficient bioprocess development. Such strategies are beneficial for industries as well as proven to be vital for effectual therapeutic protein development. Though, these techniques are well documented, but, there is scope of addition to current knowledge with novel and new approaches and it will pave new avenues in production of recombinant microbial and non-microbial proteins including secondary metabolites. In this review, we have focussed on the recent development in clone selection, various modern fermentation and purification technologies and future directions in these emerging areas. Moreover, we have also highlighted notable perspectives and challenges involved in the bioengineering of such proteins, including quality by design, gene editing and pioneering ideas. The biopharmaceutical industries continue to shift towards more flexible, automated platforms and economical product development, which in turn can help in developing the cost effective processes and affordable drug development for a large community

Bio-Based Formulations for Sustainable Applications in Agri-Food-Pharma

Currently, there is a strong enduring interest towards obtaining high-value, sustainable bio-based bioactive compounds from natural resources, as there is great demand for these compounds in various market sectors such as agriculture, food, pharma, cosmeceuticals, and others. This demand has encouraged researchers to isolate, identify and characterize novel natural bioactive compounds with potential therapeutic and commercial values with industrial importance [1]. These bioactive compounds are generally secondary metabolites (synthesized via plant biosynthetic pathways) and include polyphenols, carotenoids, flavonoids, sterols, dietary fiber, essential vitamins, coenzyme Q, phytosterols, glucosinolates and others with potential beneficial roles as nutraceuticals, surfactants and bio-stimulants. Understanding the molecular characteristics, physicochemical properties, biological activity, and stability of these bioactives under different conditions is vital for their commercial exploitation. The efficacy of these bioactives can often be improved by encapsulating them in nanobased-formulations designed for application in the agriculture, food, pharmaceutical industries. These delivery systems can be designed to increase the dispersibility, stability, bioavailability, and bioactivity imparted by the bioactives. Moreover, they may be useful for minimizing undesirable side-effects, facilitating targeted delivery to certain cells, and enhancing the shelf life of food products

Vaccine and antibody production in plants : developments and computational tools

K.K.D. and P.S. sincerely acknowledge the support and facility provided by the M.D. University, Rohtak, Haryana. P.S. acknowledges the infrastructural support from Department of Science and Technology, Govt. of India through FIST grant (Grant No. 1196 SR/FST/LS-I/2017/4).Plants as bioreactors have been widely used to express efficient vaccine antigens against viral, bacterial and protozoan infections. To date, many different plant-based expression systems have been analyzed, with a growing preference for transient expression systems. Antibody expression in diverse plant species for therapeutic applications is well known, and this review provides an overview of various aspects of plant-based biopharmaceutical production. Here, we highlight conventional and gene expression technologies in plants along with some illustrative examples. In addition, the portfolio of products that are being produced and how they relate to the success of this field are discussed. Stable and transient gene expression in plants, agrofiltration and virus infection vectors are also reviewed. Further, the present report draws attention to antibody epitope prediction using computational tools, one of the crucial steps of vaccine design. Finally, regulatory issues, biosafety and public perception of this technology are also discussed.PostprintPeer reviewe