Zeros, Poles, and Fixed Points of Meromorphic Solutions of Difference Painlevé Equations

In this paper, we mainly study the properties of transcendental meromorphic solutions f(z) of difference Painlevé equations w(z+1)w(z-1)(w(z)-1)=η(z)w2(z)-λ(z)w(z) and w(z+1)w(z-1)(w(z)-1)=η(z)w(z) and obtain precise estimations of the exponents of convergence of zeros, poles of Δf(z) and Δf(z)/f(z), and of fixed points of f(z+c) for any c∈ℂ

On Properties of Meromorphic Solutions of Certain Difference Painlevé III Equations

We mainly study the exponents of convergence of zeros and poles of difference and divided difference of transcendental meromorphic solutions for certain difference Painlevé III equations

Anticancer potentials of sinomenine from Sinomenium acutum: A mini-review

Sinomenine, an important isoquinoline from Sinomenium acutum (Menispermaceae), is currently considered as a reliable drug for treating various malignant cancers including breast cancer, colon cancer, lung cancer, liver cancer, gastric cancer, osteosarcoma cervical cancer, and esophageal cancer. The pharmacological mechanisms are probably related to induction of apoptosis and cell cycle arrest. This paper was aimed at reviewing the anticancer potential of sinomenine, which is expected to be beneficial in developing this compound as a candidate drug for cancer therapy.Keywords: Sinomenine, Anticancer, Sinomenium acutum, Traditional Chinese Medicin

‘Internet+’ comprehensive nursing training course in the post-epidemic era—an exploration of the mixed teaching mode: a randomized trial

ObjectiveTo explore the effect of the application of the ‘Internet+’ nursing teaching mode on the comprehensive teaching ‘Fundamentals of Nursing’.Trial designParallel design and convenient sampling were used to select vocational nursing students from the Nursing College of Capital Medical University.MethodsSelected students were randomly divided into two groups. The control group consisted of 30 students in Grade 2020 higher vocational nursing education (traditional teaching mode). The observation group consisted of 30 students in Grade 2021 higher vocational nursing education (Internet+ mixed teaching mode). Training assessment results, automatic learning ability, professional identity, and satisfaction were compared between the two groups.ResultsCompared with the control group, the students in the observation group scored higher in the following operation practices: venous blood sampling, intradermal injection, cardiopulmonary resuscitation (CPR), sputum aspiration, and putting on and taking off robes (84.01 ± 0.87 vs. 92.14 ± 1.23; 91.41 ± 0.82 vs. 96.86 ± 0.27; 87.56 ± 0.31 vs. 93.91 ± 2.79; 88.11 ± 0.51 vs. 93.75 ± 0.29; and 82.29 ± 0.29 vs. 90.96 ± 0.34, respectively, with p < 0.05 for all scores). The total scores for autonomous learning ability and subjective satisfaction were also higher in the observation group compared with the control group (82.98 ± 4.72 vs. 93.17 ± 5.01 and 96.67% vs. 90.00%, respectively, with p < 0.05 for all scores).ConclusionIn the post-epidemic era, the ‘Internet+ hybrid teaching mode’ was applied to comprehensive nursing teaching. This changed the traditional education mode, which focuses only on professional knowledge. The ‘Internet+’ teaching mode results showed that the professional, ideological, and political courses exhibited the same value guidance, which improved students’ independent learning ability, practical operation ability, professional identity, and satisfaction

The Euscaphis japonica genome and the evolution of malvids

Malvids is one of the largest clades of rosids, includes 58 families and exhibits remarkable morphological and ecological diversity. Here, we report a high-quality chromosome-level genome assembly for Euscaphis japonica, an early-diverging species within malvids. Genome-based phylogenetic analysis suggests that the unstable phylogenetic position of E. japonica may result from incomplete lineage sorting and hybridization event during the diversification of the ancestral population of malvids. Euscaphis japonica experienced two polyploidization events: the ancient whole genome triplication event shared with most eudicots (commonly known as the c event) and a more recent whole genome duplication event, unique to E. japonica. By resequencing 101 samples from 11 populations, we speculate that the temperature has led to the differentiation of the evergreen and deciduous of E. japonica and the completely different population histories of these two groups. In total, 1012 candidate positively selected genes in the evergreen were detected, some of which are involved in flower and fruit development. We found that reddening and dehiscence of the E. japonica pericarp and long fruit-hanging time promoted the reproduction of E. japonica populations, and revealed the expression patterns of genes related to fruit reddening, dehiscence and abscission. The key genes involved in pentacyclic triterpene synthesis in E. japonica were identified, and different expression patterns of these genes may contribute to pentacyclic triterpene diversification. Our work sheds light on the evolution of E. japonica and malvids, particularly on the diversification of E. japonica and the genetic basis for their fruit dehiscence and abscission.DATA AVAILABILITY STATEMENT : All sequences described in this manuscript have been submitted to the National Genomics Data Center (NGDC). The raw whole-genome data of E. japonica have been deposited in BioProject/GSA (https://bigd.big.ac.cn/gsa.) under the accession codes PRJCA005268/CRA004271, and the assembly and annotation data have been deposited at BioProject/GWH (https://bigd.big.ac.cn/gwh) under the accession codes PRJCA005268/GWHBCHS00000000. The raw transcriptomes data of E. japonica have been deposited in BioProject/GSA (https://bigd.big.ac.cn/gsa.) under the accession codes PRJCA005298/CRA004272.SUPPLEMENTARY MATERIAL 1: Supplementary Note 1. Chromosome number assessment. Supplementary Note 2. Whole-genome duplication identification and dating. Supplementary Note 3. Observation of E. japonica seed dispersal. Supplementary Note 4. Determination of pentacyclic triterpene substances. Figure S1. Cytogenetic analysis of E. japonica. Figure S2. Genome size and heterozygosity of E. japonica estimation using 17 k-mer distribution. Figure S3. Interchromosomal of Hi-C chromosome contact map of E. japonica genome. Figure S4. Gene structure prediction results of E. japonica and other species. Figure S5. Venn diagram shows gene families of malvids. Figure S6. Phylogenetic tree constructed by chloroplast genomes from 17 species. Figure S7. Concatenated- and ASTRAL-based phylogenetic trees. Figure S8. Ks distribution in E. japonica. Figure S9. Distributions of synonymous substitutions per synonymous site (Ks) of one-to-one orthologs identified between E. japonica and P. trichocarpa and V. vinifera. Figure S10. Population structure plot. Figure S11. Fixation index (FST) heat map among E. japonica populations. Figure S12. Phylogenetic analysis of MADS-box genes from O. sativa, A. thaliana, E. japonica, and T. cacao. Figure S13. Observation the fruit development. Figure S14. Animal seed dispersal. Figure S15. Anthocyanin biosynthesis in E. japonica fruits. Figure S16. Carotenoid accumulation and the chlorophyll degradation in E. japonica fruits. Figure S17. Expression profile of fruit dehiscence-related genes. Figure S18. Phylogenetic tree of DELLA genes obtained from six malvids species. Figure S19. Phylogenetic tree of CAD genes obtained from seven malvids species. Figure S20. Expression pattern of fruit abscission-related genes. Figure S21. Structure of pentacyclic triterpene compounds separated from Euscaphis. Figure S22. Phylogenetic tree of HMGR gene in plants. Figure S23. Phylogenetic tree of P450s gene family obtained from A. thaliana and E. japonica.SUPPLEMENTARY MATERIAL 2: Table S1. Assembled statistics of E. japonica genome. Table S2. Evaluation of E. japonica genome assembly. Table S3. Chromosome length of E. japonica. Table S4. Prediction of gene structures of the E. japonica genome. Table S5. Statistics on the function annotation of the E. japonica genome. Table S6. Non-coding RNA annotation results of E. japonica genome. Table S7. BUSCO assessment of the E. japonica annotated genome. Table S8. Statistic of repeat sequence in E. japonica genome. Table S9. Gene-clustering statistics for 17 species. Table S10. KEGG enrichment result of unique genes families of E. japonica. Table S11. Gene Ontology (GO) and KEGG enrichment result of significant shared by malvids species gene families. Table S12. Gene Ontology (GO) and KEGG enrichment result of significant expansion of E. japonica gene families. Table S13. Gene Ontology (GO) enrichment result of significant contraction of E. japonica gene families. Table S14. Statistical sampling population information. Table S15. Statistics population resequencing information. Table S16. Statistical nucleotide polymorphisms in the populations. Table S17. Candidate positive selection genes (PSGs) in the evergreen population. Table S18. Candidate positive selection genes (PSGs) in the deciduous population. Table S19. Gene Ontology (GO) enrichment result of significant PSGs in the evergreen population. Table S20. List of MADS-box genes identified in E. japonica. Table S21. Genes involved in anthocyanin biosynthesis, carotenoid biosynthesis, and chlorophyll degradation. Table S22. Identification fruit dehiscence-related genes in E. japonica. Table S23. Genes related to lignin synthesis that are highly expressed during pericarp dehiscence. Table S24. Gene expression levels (FPKMs) of fruit abscission-related genes in pericarp. Table S25. Triterpene compounds separated from Euscaphis. Table S26. Number of putative pentacyclic triterpene-related genes in the malvids species. Table S27. Identified pentacyclic triterpene synthesis-related genes in E. japonica genome. Table S28. Statistical simple sequence repeat.Fund for Excellent Doctoral Dissertation of Fujian Agriculture and Forestry University, China; Fujian Provincial Department of Science E. japonica Evolution and Selection of Ornamental Medicinal Resources, China; the Project of Forestry Peak Discipline at Fujian Agriculture and Forestry University, China; the Collection, Development and Utilization of Eascaphis konlshli Germplasm Resources; the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program and from Ghent University.https://onlinelibrary.wiley.com/journal/1365313xam2022BiochemistryGeneticsMicrobiology and Plant Patholog

Wolfberry genomes and the evolution of Lycium (Solanaceae)

AbstractWolfberry Lycium, an economically important genus of the Solanaceae family, contains approximately 80 species and shows a fragmented distribution pattern among the Northern and Southern Hemispheres. Although several herbaceous species of Solanaceae have been subjected to genome sequencing, thus far, no genome sequences of woody representatives have been available. Here, we sequenced the genomes of 13 perennial woody species of Lycium, with a focus on Lycium barbarum. Integration with other genomes provides clear evidence supporting a whole-genome triplication (WGT) event shared by all hitherto sequenced solanaceous plants, which occurred shortly after the divergence of Solanaceae and Convolvulaceae. We identified new gene families and gene family expansions and contractions that first appeared in Solanaceae. Based on the identification of self-incompatibility related-gene families, we inferred that hybridization hotspots are enriched for genes that might be functioning in gametophytic self-incompatibility pathways in wolfberry. Extremely low expression of LOCULE NUBER (LC) and COLORLESS NON-RIPENING (CNR) orthologous genes during Lycium fruit development and ripening processes suggests functional diversification of these two genes between Lycium and tomato. The existence of additional flowering locus C-like MADS-box genes might correlate with the perennial flowering cycle of Lycium. Differential gene expression involved in the lignin biosynthetic pathway between Lycium and tomato likely illustrates woody and herbaceous differentiation. We also provide evidence that Lycium migrated from Africa into Asia, and subsequently from Asia into North America. Our results provide functional insights into Solanaceae origins, evolution and diversification.</jats:p

Zeros, Poles, and Fixed Points of Meromorphic Solutions of Difference Painlevé Equations

In this paper, we mainly study the properties of transcendental meromorphic solutions ( ) of difference Painlevé equations ( + 1) ( − 1)( ( ) − 1) = ( ) 2 ( ) − ( ) ( ) and ( + 1) ( − 1)( ( ) − 1) = ( ) ( ) and obtain precise estimations of the exponents of convergence of zeros, poles of Δ ( ) and Δ ( )/ ( ), and of fixed points of ( + ) for any ∈ C

Urinary 8-oxo-7,8-dihydroguanosine as a Potential Biomarker of Aging

Depuis une dizaine d’années, grâce à de nouveaux documents sur les relations sino-soviétiques issus des archives russes et chinoises, les spécialistes réexaminent les annales diplomatiques pour compléter la compréhension des ressorts de cette relation complexe. Dieter Heinzig apporte une contribution importante à cet effort avec cette recherche méticuleuse sur les rencontres diplomatiques entre l’URSS et la Chine de 1945 à 1950. Son travail repose principalement sur des études récentes de che..

Urinary 8-oxo-7,8-dihydroguanosine as a Potential Biomarker of Aging

Background: A molecular biomarker of physiologic age, as opposed to chronologic age, is needed in clinical medicine. 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGsn) and 8-oxo-7, 8-dihydroguanosine (8-oxoGsn) are two promising aging biomarkers.Methods: A total of 1,228 healthy Chinese residents (613 males and 615 females) 2–90 years of age were randomly selected. Spot urine samples were collected, and the concentrations of 8-oxodGsn and 8-oxoGsn were measured using ultra-high-performance liquid chromatography with a triple quadrupole mass spectrometer (UPLC-MS/MS). Method validation, including accuracy, precision, linearity and quantification limit, was performed. The relationship between oxidized guanosine and age/gender was evaluated.Results: 8-oxodGsn and 8-oxoGsn were eluted at 1.61 and 1.30 min, respectively. The calibration curve was linear in the range of 0.2–500 ng/ml for both analytes. The lowest limit of quantification (LLOQ) was 0.2 ng/ml for 8-oxodGsn and 0.1 ng/ml for 8-oxoGsn. There was an age-dependent increase in the biomarkers from the 21- to 30-year-old group to the 81- to 90-year-old group in both genders. In the subjects older than 61 years of age, the levels of 8-oxodGsn as well as 8-oxoGsn in urine were much higher in females than in males. The content of 8-oxoGsn correlated more closely with age and was higher (approximately 2-fold) than that of 8-oxodGsn for a given individual.Conclusions: 8-oxodGsn and 8-oxoGsn can be easily measured by UPLC-MS/MS. Urinary 8-oxoGsn may be a potential biomarker to determine a person's physiologic age and identify individuals at high risk of developing age-associated disease

A comparison of citation disciplinary structure in science between the G7 countries and the BRICS countries

This study aims to compare the characteristics of citation disciplinary structure between the G7 countries and the BRICS countries. In this contribution, which uses about 1 million Web of Science publications and two publications years (1993 and 2013), we compare the G7 countries and the BRICS countries with regard to this type of structure. For the publication year 2013, cosine similarity values regarding the citation disciplinary structures of these countries (and of nine other countries) were used as input to cluster analysis. We also obtained cosine similarity values for a given country and its citation disciplinary structures across the two publication years. Moreover, for the publication year 2013, the within-country Jeffreys-Matusita distance between publication and citation disciplinary structure was measured. First, the citation disciplinary structures of countries depend on multiple and complex factors. It is therefore difficult to completely explain the formation and change of the citation disciplinary structure of a country. This study suggests some possible causes, whereas detailed explanations might be given by future research. Second, the length of the citation window used in this study is three years. However, scientific disciplines differ in their citation practices. Comparison between citations across disciplines using the same citation window length may affect the citation discipline structure results for some countries. First, the results of this study are based on the WoS database. However, in this database some fields are covered to a greater extent than others, which may affect the results for the citation discipline structure for some studied countries. In future research, we might repeat this study using another database (like Scopus) and, in that case, we would like to make comparisons between the two outcomes. Second, the use of a constant journal set yielded that a large share of the journals covered by WoS year 2013 is ignored in the study. Thus, disciplinary structure is studied based on a quite restricted set of publications. The three mentioned limitations should be kept in mind when the results of this study are interpreted. Disciplinary structure on country level is a highlighted topic for the S&T policy makers, especially for those come from developing countries. This study observes the disciplinary structure in the view of academic impact, and the result will provide some evidence to make decision for the discipline strategy and funding allocation. Besides, Jeffreys-Matusita distance is introduced to measure the similarity of citation disciplinary structure and publication disciplinary structure. By applying this measure, some new observations were drawn, for example, "Based on the comparison of publication disciplinary structure and citation disciplinary structure, the paper finds most BRICS counties have less impact with more publications". The outcome of the cluster analysis indicates that the G7 countries and BRICS countries are quite heterogeneous regarding their citation disciplinary structure. For a majority of the G7 countries, the citation disciplinary structure tend to be more stable compared to BRICS countries with regard to the years 1993 and 2013. Most G7 countries, with United States as an exception, turned out to have lower values on the Jeffreys-Matusita distance than BRICS countries, indicating a higher degree of heterogeneity between the publication and the citation disciplinary structure for the latter countries. In other words, BRICS countries still receive much less citations in most disciplines than their publication output would suggest. G7 countries can still expect more citations than is to be expected based on their publication output, thereby generating relatively more impact than BRICS countries