Clinical and Virological Study of Dengue Cases and the Members of Their Households: The Multinational DENFRAME Project

Dengue is the most important mosquito-borne viral disease in humans. This disease is now endemic in more than 100 countries and threatens more than 2.5 billion people living in tropical countries. It currently affects about 50 to 100 million people each year. It causes a wide range of symptoms, from an inapparent to mild dengue fever, to severe forms, including dengue hemorrhagic fever. Currently no specific vaccine or antiviral drugs are available. We carried out a prospective clinical study in South-East Asia and Latin America, of virologically confirmed dengue-infected patients attending the hospital, and members of their households. Among 215 febrile dengue subjects, 177 agreed to household investigation. Based on our data, we estimated the proportion of dengue-infected household members to be about 45%. At the time of the home visit, almost three quarters of (29/39) presented an inapparent dengue infection. The proportion of inapparent dengue infection was higher in South-East Asia than in Latin America. These findings confirm the complexity of dengue disease in humans and the need to strengthen multidisciplinary research efforts to improve our understanding of virus transmission and host responses to dengue virus in various human populations

Effects of the Combined Application of Ethephon and Gibberellin on Growth of Rice (Oryza sativa L.) Seedlings

Improvement of early seedling growth, such as seedling emergence and vigor is one of the most important agronomic traits in direct seeding rice cultivation. The effects of two plant growth regulators (PGRs), gibberellic acid (GA3) and ethephon (ET), on seedling growth under flooded soil conditions at different temperatures and water depths were investigated. The PGRs were applied during the seed soaking process. A single treatment with GA3 or ET increased seedling growth. However, combined application of GA3 and ET was more effective than that of GA3 or ET alone in many cases at both growing temperatures (15 and 20ºC). The growth of different organs in the rice seedlings, such as the coleoptiles, first leaves, and second leaves was also increased by PGR treatment. The nitrogen concentration of the shoot and the ratio of shoot dry weight to shoot length did not differ significantly among the treatments. The results of our study show that rice seedling growth in direct seeding cultivation may be improved by treatment with GA3 and ET in combination

Individual Sick Fir Tree (Abies mariesii) Identification in Insect Infested Forests by Means of UAV Images and Deep Learning

Insect outbreaks are a recurrent natural phenomenon in forest ecosystems expected to increase due to climate change. Recent advances in Unmanned Aerial Vehicles (UAV) and Deep Learning (DL) Networks provide us with tools to monitor them. In this study we used nine orthomosaics and normalized Digital Surface Models (nDSM) to detect and classify healthy and sick Maries fir trees as well as deciduous trees. This study aims at automatically classifying treetops by means of a novel computer vision treetops detection algorithm and the adaptation of existing DL architectures. Considering detection alone, the accuracy results showed 85.70% success. In terms of detection and classification, we were able to detect/classify correctly 78.59% of all tree classes (39.64% for sick fir). However, with data augmentation, detection/classification percentage of the sick fir class rose to 73.01% at the cost of the result accuracy of all tree classes that dropped 63.57%. The implementation of UAV, computer vision and DL techniques contribute to the development of a new approach to evaluate the impact of insect outbreaks in forest

Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes

<p>Lysosomes are thought to be the major intracellular compartment for the degradation of macromolecules. We recently identified a novel type of autophagy, RNautophagy, where RNA is directly taken up by lysosomes in an ATP-dependent manner and degraded. However, the mechanism of RNA translocation across the lysosomal membrane and the physiological role of RNautophagy remain unclear. In the present study, we performed gain- and loss-of-function studies with isolated lysosomes, and found that SIDT2 (SID1 transmembrane family, member 2), an ortholog of the <i>Caenorhabditis elegans</i> putative RNA transporter SID-1 (systemic RNA interference deficient-1), mediates RNA translocation during RNautophagy. We also observed that SIDT2 is a transmembrane protein, which predominantly localizes to lysosomes. Strikingly, knockdown of <i>Sidt</i>2 inhibited up to ˜50% of total RNA degradation at the cellular level, independently of macroautophagy. Moreover, we showed that this impairment is mainly due to inhibition of lysosomal RNA degradation, strongly suggesting that RNautophagy plays a significant role in constitutive cellular RNA degradation. Our results provide a novel insight into the mechanisms of RNA metabolism, intracellular RNA transport, and atypical types of autophagy.</p