303 research outputs found
Plasma-Coated Polycaprolactone Nanofibers with Covalently Bonded Platelet-Rich Plasma Enhance Adhesion and Growth of Human Fibroblasts.
Biodegradable nanofibers are extensively employed in different areas of biology and medicine, particularly in tissue engineering. The electrospun polycaprolactone (PCL) nanofibers are attracting growing interest due to their good mechanical properties and a low-cost structure similar to the extracellular matrix. However, the unmodified PCL nanofibers exhibit an inert surface, hindering cell adhesion and negatively affecting their further fate. The employment of PCL nanofibrous scaffolds for wound healing requires a certain modification of the PCL surface. In this work, the morphology of PCL nanofibers is optimized by the careful tuning of electrospinning parameters. It is shown that the modification of the PCL nanofibers with the COOH plasma polymers and the subsequent binding of NH(2) groups of protein molecules is a rather simple and technologically accessible procedure allowing the adhesion, early spreading, and growth of human fibroblasts to be boosted. The behavior of fibroblasts on the modified PCL surface was found to be very different when compared to the previously studied cultivation of mesenchymal stem cells on the PCL nanofibrous meshes. It is demonstrated by X-ray photoelectron spectroscopy (XPS) that the freeze-thawed platelet-rich plasma (PRP) immobilization can be performed via covalent and non-covalent bonding and that it does not affect biological activity. The covalently bound components of PRP considerably reduce the fibroblast apoptosis and increase the cell proliferation in comparison to the unmodified PCL nanofibers or the PCL nanofibers with non-covalent bonding of PRP. The reported research findings reveal the potential of PCL matrices for application in tissue engineering, while the plasma modification with COOH groups and their subsequent covalent binding with proteins expand this potential even further. The use of such matrices with covalently immobilized PRP for wound healing leads to prolonged biological activity of the immobilized molecules and protects these biomolecules from the aggressive media of the wound
Characteristics and in vitro response of thin hydroxyapatite-titania films produced by plasma electrolytic oxidation of Ti alloys in electrolytes with particle additions
The enhancement of the biological properties of Ti by surface doping with hydroxyapatite (HA) is of great significance, especially for orthodontic applications. This study addressed the effects of HA particle size in the electrolyte suspension on the characteristics and biological properties of thin titania-based coatings produced on Tiβ6Alβ4V alloy by plasma electrolytic oxidation (PEO). Detailed morphological investigation of the coatings formed by a single-stage PEO process with two-step control of the electrical parameters was performed using the Minkowski functionals approach. The surface chemistry was studied by glow discharge optical emission spectroscopy and Fourier transform infrared spectroscopy, whereas mechanical properties were evaluated using scratch tests. The biological assessment included in vitro evaluation of the coating bioactivity in simulated body fluid (SBF) as well as studies of spreading, proliferation and osteoblastic differentiation of MC3T3-E1 cells. The results demonstrated that both HA micro- and nanoparticles were successfully incorporated in the coatings but had different effects on their surface morphology and elemental distributions. The micro-particles formed an irregular surface morphology featuring interpenetrated networks of fine pores and coating material, whereas the nanoparticles penetrated deeper into the coating matrix which retained major morphological features of the porous TiO2 coating. All coatings suffered cohesive failure in scratch tests, but no adhesive failure was observed; moreover doping with HA increased the coating scratch resistance. In vitro tests in SBF revealed enhanced bioactivity of both HA-doped PEO coatings; furthermore, the cell proliferation/morphometric tests showed their good biocompatibility. Fluorescence microscopy revealed a well-organised actin cytoskeleton and focal adhesions in MC3T3-E1 cells cultivated on these substrates. The cell alkaline phosphatase activity in the presence of ascorbic acid and Ξ²-glycerophosphate was significantly increased, especially in HA nanoparticle-doped coatings
ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π΄Π΅ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΊΠΎΠ½ΡΠ°ΠΊΡΠ° ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°Ρ - ΠΊΠΎΡΡΠ½Π°Ρ ΡΠΊΠ°Π½Ρ
Aim: a) to develop the method for surface modification of polytetrafluoroethylene (PTFE) by applying a nanostructured metallic or ceramic coating, b) to study the possibility of using this obtained structures with different coatings as an experimental model for analysis of the fine interactions in the contact implant-tissue zone. The study demonstrates that the surface modification of PTFE by magnetron sputtering of nano-Ti and Ti-Ca-P-C-O-N leads to increased integration potential of materials. Data obtained indicate that the designed constructions can be successfully used as an experimental model for studying the interactions of implant - tissue interface elements of periimplant area.Π Π·Π°Π΄Π°ΡΠΈ ΡΠ°Π±ΠΎΡΡ Π²Ρ
ΠΎΠ΄ΠΈΠ»ΠΎ: Π°) ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ° ΠΌΠ΅ΡΠΎΠ΄Π° ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΏΠΎΠ»ΠΈΡΠ΅ΡΡΠ°ΡΡΠΎΡΡΡΠΈΠ»Π΅Π½Π° (ΠΠ’Π€Π) ΠΏΡΡΠ΅ΠΌ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΡ Π½Π°Π½ΠΎΡΡΡΡΠΊΡΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π»Π»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ»ΠΈ ΠΊΠ΅ΡΠ°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΠΊΡΡΡΠΈΡ; Π±) ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠΉ ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΏΠΎ ΡΠΎΡΡΠ°Π²Ρ ΠΏΠΎΠΊΡΡΡΠΈΡΠΌΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠ½ΠΊΠΈΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΊΠΎΠ½ΡΠ°ΠΊΡΠ° ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°Ρ - ΡΠΊΠ°Π½Π΅Π²ΡΠ΅ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΠΏΠ΅ΡΠΈΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π·ΠΎΠ½Ρ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π»ΠΎ, ΡΡΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΠ’Π€Π ΠΏΡΡΠ΅ΠΌ ΠΌΠ°Π³Π½Π΅ΡΡΠΎΠ½Π½ΠΎΠ³ΠΎ Π½Π°ΠΏΡΠ»Π΅Π½ΠΈΡ Π½Π°Π½ΠΎΠΏΠΎΠΊΡΡΡΠΈΠΉ Ti ΠΈ Ti-Ca-P-C-O-N ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π° ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ². ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠ΅ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΈ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ Ρ ΡΡΠΏΠ΅Ρ
ΠΎΠΌ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΠΉΡΠ° ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°Ρ - ΡΠΊΠ°Π½Π΅Π²ΡΠ΅ ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΠΏΠ΅ΡΠΈΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π·ΠΎΠ½Ρ
HETEROGENEOUS Fe3O4/h-BN NANOSTRUCTURES MANUFACTURING VIA POLYOL SYNTHESIS
Fe3O4/h-BN heterogeneous structures were synthesized via polyol process and character-ized. Comparison of the structure of the unsupported Fe3O4 nanoparticles and those obtained over the h-BN surfaces was conducted.Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° ΠΏΡΠΈ ΡΠΈΠ½Π°Π½ΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠ΅ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΠ°ΡΡΠ½ΠΎΠ³ΠΎ Π€ΠΎΠ½Π΄Π° (ΡΠΎΠ³Π»Π°ΡΠ΅Π½ΠΈΠ΅ β 20-79-10286)
Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds
Due to their good mechanical stability compared to gelatin, collagen or polyethylene glycol nanofibers and slow degradation rate, biodegradable poly-epsilon-caprolactone (PCL) nanofibers are promising material as scaffolds for bone and soft-tissue engineering. Here, PCL nanofibers were prepared by the electrospinning method and then subjected to surface functionalization aimed at improving their biocompatibility and bioactivity. For surface modification, two approaches were used: (i) COOH-containing polymer was deposited on the PCL surface using atmospheric pressure plasma copolymerization of CO2 and C2H4, and (ii) PCL nanofibers were coated with multifunctional bioactive nanostructured TiCaPCON film by magnetron sputtering of TiC-CaO-Ti3POx target. To evaluate bone regeneration ability in vitro, the surface-modified PCL nanofibers were immersed in simulated body fluid (SBF, 1x) for 21 days. The results obtained indicate different osteoblastic and epithelial cell response depending on the modification method. The TiCaPCON-coated PCL nanofibers exhibited enhanced adhesion and proliferation of MC3T3-E1 cells, promoted the formation of Ca-based mineralized layer in SBF and, therefore, can be considered as promising material for bone tissue regeneration. The PCL-COOH nanofibers demonstrated improved adhesion and proliferation of IAR-2 cells, which shows their high potential for skin reparation and wound dressing
AGGREGATIVE STABILITY OF h-BN HYDROSOL IN THE PRESENCE OF NaCl
The aggregative stability of h-BN hydrosol in the presence of NaCl solution was studied by DLS and turbidimetry. NaCl was demonstrated to be indifferent with respect to hydrosol particles.The threshold concentration of NaCl below which h-BN hydrosol remain relatively stable was determined.Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° ΠΏΡΠΈ ΡΠΈΠ½Π°Π½ΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠ΅ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΠ°ΡΡΠ½ΠΎΠ³ΠΎ Π€ΠΎΠ½Π΄Π° (ΡΠΎΠ³Π»Π°ΡΠ΅Π½ΠΈΠ΅ β 20β19β00120)
Development of Heterogeneous Nanostructures Based on Bimetallic Nanoparticles and Two-Dimensional Boron Nitride
The development of industry leads to the emergence of new, important environmental problems. This makes it necessary to develop new types of adsorbents. Important properties of such materials are their inertia, as well as the possibility of its removal from the water for further processing.Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° ΠΏΡΠΈ ΡΠΈΠ½Π°Π½ΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠ΅ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΠ°ΡΡΠ½ΠΎΠ³ΠΎ Π€ΠΎΠ½Π΄Π° (ΡΠΎΠ³Π»Π°ΡΠ΅Π½ΠΈΠ΅ β 20-79-10286)
SYNTHESIS AND PROPERTIES OF HETEROSTRUCTURED Ag/MgO/BN CATALYSTS
Microparticles of hexagonal boron nitride with a trace Mg content were subjected to a high-temperature annealing on air. As the result formation of hexagonal-shaped surface defects decorated by MgO nanoparticles (NPs) occurred. Formation of Ag over the surfaces of the obtained substrate was conducte.Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° ΠΏΡΠΈ ΡΠΈΠ½Π°Π½ΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠ΅ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΠ°ΡΡΠ½ΠΎΠ³ΠΎ Π€ΠΎΠ½Π΄Π° (ΡΠΎΠ³Π»Π°ΡΠ΅Π½ΠΈΠ΅ β 20-79-10286)
Research of the Effect of Synthesis Temperature on the Formation of the MoS2 Structure on the Surface of Activated Carbon
Two-dimensional 2D materials have attracted wide attention in recent years due to their unique physicochemical properties, such as high specific surface area, electromagnetic properties, etc.Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° ΠΏΡΠΈ ΡΠΈΠ½Π°Π½ΡΠΎΠ²ΠΎΠΉ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΠ΅ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΠ°ΡΡΠ½ΠΎΠ³ΠΎ Π€ΠΎΠ½Π΄Π° (ΡΠΎΠ³Π»Π°ΡΠ΅Π½ΠΈΠ΅ β 21-49-00039)
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