Formulation and Characterization of Phytostanol Ester Solid Lipid Nanoparticles for the Management of Hypercholesterolemia: An ex vivo Study.

Background: Phytostanols are naturally occurring compounds that reduce blood cholesterol levels significantly. However, their aqueous insolubility poses formulation challenges. Aim: To formulate and characterize solid lipid nanoparticle carriers for phytostanol esters to enhance the bioavailability of phytostanols. Methods: Phytostanol ester solid lipid nanoparticles were formulated by the microemulsion method. They were characterized for particle size distribution, polydispersity index, shape, surface charge, entrapment efficiency, stability, chemical structure, and thermal properties. The uptake of the formulation by cell lines, HepG2 and HT-29, and its effect on cell viability were evaluated. Results: The formulation of solid lipid nanoparticles was successfully optimised by varying the type of lipids and their concentration relative to that of surfactants in the present study. The optimised formulation had an average diameter of (171 ± 9) nm, a negative surface charge of (− 23.0 ± 0.8) mV and was generally spherical in shape. We report high levels of drug entrapment at (89 ± 5)% in amorphous form, drug loading of (9.1 ± 0.5)%, nanoparticle yield of (67 ± 4)% and drug excipient compatibility. The biological safety and uptake of the formulations were demonstrated on hepatic and intestinal cell lines. Conclusion: Phytostanol ester solid lipid nanoparticles were successfully formulated and characterized. The formulation has the potential to provide an innovative drug delivery system for phytostanols which reduce cholesterol and have a potentially ideal safety profile. This can contribute to better management of one of the main risk factors of cardiovascular disease

Rapid silviculture appraisal to characterise stand and determine silviculture priorities of community forests in Nepal

Published online: 7 September 2016Community forestry in Nepal is an example of a successful participatory forest management program. Developments in community forestry in four decades have focused on the social and governance aspects with little focus on the technical management of forests. This paper presents a silviculture description of community forests and provides silviculture recommendations using a rapid silviculture appraisal (RSA) approach. The RSA, which is a participatory technique involving local communities in assessing forests and silviculture options, is a simple and costeffective process to gather information and engage forest users in the preparation of operational plans that are relevant to their needs. The RSA conducted on selected community forests in Nepal’s Mid-hills region shows that forests are largely comprised of dominant crowns of one or two species. The majority of studied community forests have tree densities below 500 stems per hectare as a consequence of traditional forest management practices but the quality and quantity of the trees for producing forest products are low. Silviculture options preferred by forest users generally are those which are legally acceptable, doable with existing capacities of forest users and generate multiple forest products. For sustainable production of multiple forest products, the traditional forest management practices have to be integrated with silviculture-based forest management system.Edwin Cedamon, Ian Nuberg, Govinda Paudel, Madan Basyal, Krishna Shrestha, Naya Paude

Pathways to forest wealth in Nepal

Nepal is one of the leading countries embracing community forestry with about 45% of households being members of community forest user groups. However, there has been a failure to deliver the full potential of forest wealth because of a lack of proper silvicultural management, a constraining policy environment and a complex socio-institutional context. Meanwhile, mid-hill agriculture has not kept pace with the changing economy and out-migration. Food insecurity is rife in a landscape of under-utilised forests and under-utilised land. Australian development assistance between 1978 and 2006 supported the establishment of 21 000 ha of community forests and significant contributions to community forest institutions. In the light of the under-performance of this sector, the Australian Centre for International Agricultural Research established the project Enhancing Food Security and Livelihoods through Agroforestry and Community Forestry in Nepal, locally known as EnLiFT, which ran from 2013 to 2018. This paper aims to explain Australia’s contribution to Nepal’s forestry, with a focus on more recent achievements supporting pathways to realise the potential wealth in Nepal’s forests. It begins with an outline of the early Australian support and origins of community forestry in Nepal via the Nepal–Australia Forestry Project, and then the current status of community forestry. It then describes the research process of the EnLiFT project starting with new conceptual models and methods such as: (1) the Pathways Approach to link forest and food security; (2) the EnLiFT Bioeconomic Model of the Farm-Forest Interface; (3) the Silvo-Institutional Model for Scientific Forest Management; (4) Active and Equitable Forest Management; (5) Rapid Silvicultural Appraisal; (6) the Strategic and Inclusive Planning process and (7) EnLiFT Policy Labs. We also highlight many significant development impacts of EnLiFT. The demonstration and training of silvicultural methods released considerable forest wealth into the community. This occurred around the time of the 2015 earthquake when timber was in need for reconstruction. It was also associated with the re-vitalisation of a defunct sawmill by facilitation of community-private partnership. It was responsible for turning the public debate from resistance to acceptance of scientific forest management. It also developed inclusive planning processes for the revision of operational plans of community forests. On privately owned land, EnLiFT demonstrated: (1) the possibility for marked and rapid changes in livelihoods from relatively simple agroforestry interventions based on horticultural commodities and tree fodders; (2) an even greater potential for livelihood enhancement through private forestry and (3) articulated the current institutional and regulatory constraints on sale of trees from private land. We conclude by highlighting the contributions of EnLiFT in policy debate which led to policy outcomes that further the improvement of community forestry, agroforestry and bringing under-utilised land back into productive use.This work was supported by the Australian Centre for International Agricultural Research [FST/2011/076]

How understanding of rural households' diversity can inform agroforestry and community forestry programs in Nepal

Socio-economic diversity can help to bring about innovative development in agroforestry practices. The diversity of households in the mid-Nepal hills was analysed using survey data from 521 randomly selected households in six villages. A cluster analysis derived the following household typology based on socio-economic variables—Type 1: resource-poor Brahmin/Chhetri; Type 2: resource-poor Janajati; Type 3: resource-rich mixed-caste households; Type 4: resource-rich Brahmin/Chhetri; Type 5: resource-rich Janajati; Type 6: resource-poor Dalit households. The analysis revealed that social status (caste/ethnicity), household status on foreign employment and landholding are strong predictors of household segmentation in rural Nepal. This paper suggests revision of existing wellbeing ranking approaches using these socio-economic variables for more inclusive and equitable agroforestry and community forestry outcomes.E. Cedamon, I. Nuberg and K.K. Shresth

Village Baseline Study: Site Analysis Report for Rupandehi, Madhuri Village, Nepal

This is the report of the village baseline study of Madhuri Village in the CCAFS benchmark site of Rupandehi, Nepal conducted from June 5-9, 2011 to complement an earlier household baseline survey done in the same village. Madhuri is located in the fertile area of the Indo-Gangetic Plain in Nepal yet faces challenges due to increasing populations, encroachment on forests, decreasing soil fertility, limited agriculture and animal productivity, lack of opportunities, and variable climatic conditions. Its circumstances present manageable opportunities to prevent an increase in food insecurity and further degradation natural resources. Madhuri has yet to incur any food or environmental crises

Village Baseline Study: Site Analysis Report for Vaishali, Mukundpur Village, India

The baseline effort consists of three components – a household survey, village study and organisational survey. The household baseline survey, a quantitative questionnaire on basic indicators of welfare, information sources, livelihood/agriculture/natural resource management strategies, needs and uses of climate and agricultural-related information and current risk management, mitigation and adaptation practices, was implemented by CCAFS partners in 35 sites (245 villages) with nearly 5,000 households in 12 countries to date. CCAFS partners are implementing village baseline studies (VBS) and organisational surveys in one out of the seven villages within each CCAFS site where the household survey was implemented. The plan is to revisit these villages in roughly 5 years, and again in 10 years, to monitor what changes have occurred since the baseline was carried out. The goal is not to attribute these changes to the program, but to be able to assess what kinds of changes have occurred and whether these changes are helping villages adapt to, and mitigate, climate change. The focus of this site analysis report is the VBS. To date, seventeen VBS were conducted. The VBS aims to provide baseline information at the village level about some basic indicators of natural resource utilisation, organisational landscapes, information networks for weather and agricultural information, as well as mitigation baseline information, which can be compared across sites and monitored over time

MuPix7 - A fast monolithic HV-CMOS pixel chip for Mu3e

The MuPix7 chip is a monolithic HV-CMOS pixel chip, thinned down to 50 \mu m. It provides continuous self-triggered, non-shuttered readout at rates up to 30 Mhits/chip of 3x3 mm^2 active area and a pixel size of 103x80 \mu m^2. The hit efficiency depends on the chosen working point. Settings with a power consumption of 300 mW/cm^2 allow for a hit efficiency >99.5%. A time resolution of 14.2 ns (Gaussian sigma) is achieved. Latest results from 2016 test beam campaigns are shown.Comment: Proceedingsfor the PIXEL2016 conference, submitted to JINST A dangling reference has been removed from this version, no other change

Applications of single-tree selection guideline folllowing a DBq approach on Nepal's community forests

There is growing interest by forest users, government forest officers and policy makers on maximising forest goods and livelihood provisions from community forestry in a sustainable manner. However, the way several mature community forests are currently managed based on selection, e.g. negative thinning and crown thinning, is questionable as it results to decline in forest stock, timber quality and regeneration. To assist forest users in managing their community forests, an action research was implemented in Kavre and Lamjung to manage planted Pine (Pinus spp.) and naturallyregenerated Sal (Shorea robusta) through selection system. This paper describes the q-factor and its relevance for sustainable community forest management in Nepal. The simple guideline for selection system introduced to 30 community forest users groups in six sites are presented for wider adoption and policy recommendation.E. Cedamon, G. Paudel, M. Basyal, I. Nuberg and K. K. Shresth

Q-factors is a useful guide for selection silviculture on Nepal's community forests

There is growing interest by forest users, government forestry officers and policy makers on maximising forest goods and livelihood provisions from community forestry in a sustainable manner. However the way several mature community forests are currently managed based on selection, e.g. negative thinning and crown thinning is questionable as it results to decline in forest stock, timber quality and regeneration. To assist forest users in managing their community forests, an action research has been implemented in Kavre and Lamjung to manage planted Pine (Pinus spp.) and naturallyregenerated Sal (Shorea robusta) through selection system. This paper describes what is q-factor and its relevance for sustainable community forest management in Nepal. The simple guideline for selection system introduced to 30 community forest users groups in six sites are presented for wider adoption and policy recommendation.Edwin Cedamon, Govinda Paudel, Madan Basyal, Ian Nuberg and Krishna K Shresth

Study of size mismatches effect on transport properties in Cu-Sb and Cu-Sn liquid alloys

We report a formalism that connects thermodynamics and transport properties such as viscosity and diffusion coefficient which has been used to calculate the concentration dependence of free energy of mixing, concentration-concentration fluctuations in the long wavelength limit and concentration dependence of diffusion and viscosity in Cu-Sb and Cu-Sn binary liquid alloys at 1190K and 1400K respectively with aid of size effect and no size effect. Our calculations show that a reasonable degree of chemical order exists in both alloys system. It can be concluded that size mismatch has more effects on the transport properties of Cu-Sb hetero-coordinated system with greater size ratio than Cu-Sn hetero-coordinated system. DOI: http://dx.doi.org/10.3126/bibechana.v12i0.11781 BIBECHANA 12 (2015) 96-10