STUDY OF VIBRATION SUPPRESSION SYSTEM OF HIGH ALTITUDE PLATFORM BASED ON MR DAMPER

Based on the high attitude platform of building slabstone-installing robot with independent development,research was carried out about multi-DIM vibration. The paper firstly constructs the system with two degrees of freedom parallel mechanism as the main body and rotating MR damper as actuator. The dynamics equation of the vibration system is established by using Lagrange equation. The paper analyzes the vibration characteristics and designs a kind of rotary MR damper based on a shear mode of magneto rheological fluid. The mechanical characteristics of MR damper is analyzed. The kinematics and kinetic analysis of parallel mechanism is done. The multi-DIM vibration suppression works quickly based on the position of platform according to the kinematics equation. Finally the simulation experiment of this system of multi-DIM vibration suppression is introduced with the aid of to calculate and analysis whether the model is effective and feasible

Development of colloidal gold immunochromatographic strips for detection of Riemerella anatipestifer.

Riemerella anatipestifer is one of the most important bacterial pathogen of ducks and causes a contagious septicemia. R. anatipestifer infection causes serositis syndromes similar to other bacterial infections in ducks, including infection by Escherichia coli, Salmonella enterica and Pasteurella multocida. Clinically differentiating R. anatipestifer infections from other bacterial pathogen infections is usually difficult. In this study, MAb 1G2F10, a monoclonal antibody against R. anatipestifer GroEL, was used to develop a colloidal gold immunochromatographic strip. Colloidal gold particles were prepared by chemical synthesis to an average diameter of 20 ± 5.26 nm by transmission electron microscope imaging. MAb 1G2F10 was conjugated to colloidal gold particles and the formation of antibody-colloidal gold conjugates was monitored by UV/Vis spectroscopy. Immunochromatographic strips were assembled in regular sequence through different accessories sticked on PVC plate. Strips specifically detected R. anatipestifer within 10 min, but did not detect E. coli, S. enterica and P. multocida. The detection limit for R. anatipestifer was 1 × 10(6) colony forming units, which was 500 times higher than a conventional agglutination test. Accuracy was 100% match to multiplex PCR. Assay stability and reproducibility were excellent after storage at 4°C for 6 months. The immunochromatographic strips prepared in this study offer a specific, sensitive, and rapid detection method for R. anatipestifer, which is of great importance for the prevention and control of R. anatipestifer infections

Colloidal gold immunochromatographic strips showed negative results for <i>Escherichia coli</i>, <i>Salmonella enterica</i> and <i>Pasteurella multocida</i>.

<p>1: <i>E</i>. <i>coli</i> reference strain CVCC 1547; 2–3: <i>E</i>. <i>coli</i> serotype O1 strains APEC O1 and DE171; 4–5: <i>E</i>. <i>coli</i> serotype O2 strains DE14 and DE17; 6: <i>E</i>. <i>coli</i> serotype O18 strain CE66; 7–8: <i>E</i>. <i>coli</i> serotype O78 strains DE48 and DE56; 9–13: <i>S</i>. <i>enterica</i> strains CVCC1805, CAU0118, JXb1, CVCC 3384, and CVCC 519 respectively; 14: <i>P</i>. <i>multocida</i> strain CVCC 493.</p

UV/Vis spectra of colloidal gold and the antibody-gold conjugate.

<p>Blue: colloidal gold solution; green: the antibody-colloidal gold conjugate.</p

Specificity and sensitivity of colloidal gold immunochromatographic strip after 4°C storage.

<p>^a Simultaneously analyzed: 5 positive samples.</p><p>^b Simultaneously analyzed: 5 negative samples.</p><p>Experiments were repeated 3 times. +, Positive result; -, Negative result.</p><p>Specificity and sensitivity of colloidal gold immunochromatographic strip after 4°C storage.</p

Colloidal gold immunochromatographic strips showed positive results for different serotypes of <i>R</i>. <i>anatipestifer</i> strains.

<p>1–12: <i>R</i>. <i>anatipestifer</i> serotype 1 strains CH3, CH1, CQ1, CQ3, CQ4, CQ5, JY4, YXb12, NN2, NJ1, NJ4 and YL4 respectively; 13–23: <i>R</i>. <i>anatipestifer</i> serotype 2 strains JY1, SC2, NJ3, Yb2, Th4, YXb1, NN1, NN5, GD3, GD4 and GD5 respectively; 24: <i>R</i>. <i>anatipestifer</i> serotype 6 strain P2123; 25: <i>R</i>. <i>anatipestifer</i> serotype 8 strain D26220; 26–28: <i>R</i>. <i>anatipestifer</i> serotype 10 strains YXb11, HXb2 and YXL1; 29: <i>R</i>. <i>anatipestifer</i> serotype 12 strain 8785; 30–31: <i>R</i>. <i>anatipestifer</i> serotype 15 strains D743 and NN6; 32–33: Serotype not determined <i>R</i>. <i>anatipestifer</i> strains JY6 and GD6. 34: <i>R</i>. <i>anatipestifer</i> serotype 1 strain WJ4 (CGMCC 5264).</p

Transmission electron microscopy image of gold nanoparticles.

<p>Particles had varying sizes and shapes with an average diameter of 20±5.26 nm.</p