HL-60ヒト前骨髄性白血病細胞の分化と増殖に及ぼすドコサヘキサエン酸結合型リン脂質の影響

Effects of docosahexaenoic acid (DHA)-containing phospholipids (DHA-PLs) on retionic acid (RA)-induced cell differentiation and growth inhibition of the human promyelocytic leukemia cell line, HL-60 were investigated. Cell differentiation judged from NBT reduction assay reached to 50~60% by incubation with 12.5μM DHA-containing phosphatidylcholines (DHA-PCs) prior to the addition of RA. Differentiation without DHA-PCs remained at 39.2%. By prior incubation with DHA-containing phosphatidylethanolamines (DHA-PEs), differentiation was 70% at 12.5μM. DHA-PEs induced NBT reducing activity to as much as 20~30%, and inhibited cell growth in the absence of RA. Activity for promoting cell differentiation differed according to DHA-PL molecular species. 1-Palmitoyl (or oleoyl)-2-docosahexaenoyl-sn-glycero-3-phosphocholine (or phosphoethanolamine) was found most effective molecular species for enhancing RA-induced cell differentiation.ヒト前骨髄性白血病細胞（HL-60細胞）の分化と増殖に及ぼすドコサヘキサエン酸 (DHA) 結合型リン脂質の影響について検討した。分化誘導剤であるレチノイン酸 (RA) の単独添加に比べ，DHA結合型ホスファチジルコリンでインキュベーションした後にRAを添加することで分化率が有意に上昇した。このようなRAの分化誘導能に対する促進効果は，sn-1位がパルミチン酸又はオレイン酸，sn-2位がDHAである分子種において顕著であった。同一分子種のホスファチジルエタノールアミン (PE) の添加では，ホスファチジルコリンに比べより大きな分化誘導促進効果がみられ，DHA結合型PEのみを単独添加した場合においても，強い細胞増殖抑制作用とNBT還元能の上昇がみられた。以上の結果より，HL-60細胞の分化誘導および増殖抑制に対して，1-palmitoyl又は1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamineが最も効果的であった

Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60

The apoptosis-inducing effect of fucoxanthin on human leukemic HL-60 cells was investigated. Fucoxanthin, which was obtained from the brown alga Undaria pinnatifida, inhibited the proliferation of HL-60 cells and induced DNA fragmentation on agarose gel electrophoresis, a typical characteristic feature of apoptotic cells. The results of sandwich ELISA using anti-biotin-antibody and anti-DNA-antibody also demonstrated DNA fragmentation in accordance with the increase in fucoxanthin concentration and incubation time. Since the level of DNA fragmentation did not increase after 24 h incubation, fucoxanthin appeared to have some effect on cell cycle. In contrast, β-carotene did not show an apoptosis-inducing effect on HL-60 cells. These results suggested that a carotenoid structure might be crucial for inducing apoptosis.Not

Carcinogenesis vol.19 no.3 pp.425–431, 1998 Chemoprevention of 4-nitroquinoline 1-oxide-induced oral carcinogenesis by citrus auraptene in rats

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