Phosphorus variations in Lake Erie and its Major Tributaries

As a recurring symptom of eutrophication in Lake Erie, massive blooms of harmful algae pose a threat to safe drinking water supply and recreational water use. Causes of the recent re-eutrophication in the lake include changes in the tributary phosphorus loading and/or increases in the internal nutrient loading potentially mediated by the colonization of zebra and quagga mussels. This study is to investigate the phosphorus variations in different nearshore locations of the lake and its major tributaries for a better understanding of the lake\u27s trophic conditions. A total of 21 water samples were collected from Lake Erie and its major tributaries from Detroit Michigan to Buffalo New York. These samples were filtered and measured on phosphorus before and after digestion to determine the concentrations of soluble reactive phosphorus (SRP) and total dissolved phosphorus (TDP), using an automated discrete analyzer. Our results showed that the average concentrations of SRP and TDP in its major tributaries were significantly greater than those in the lake. The concentrations of SRP and TDP in tributaries were 0.003-0.118 and 0.002-0.112 mg/L, respectively. The concentrations of SRP and TDP in the lake were 0.00-0.01 and 0.003-0.014 mg/L, respectively.https://engagedscholarship.csuohio.edu/u_poster_2017/1016/thumbnail.jp

Processing of highly filled polyurethane elastomers by reaction injection moulding

This study is to investigate the fabrication of filled polyurethane elastomer tiles by the reaction injection moulding (RIM) process. The base matrix is composed of a crosslinked polyurethane elastomer formed by the reaction between a diisocyanate (MDI) and a polyol (polytetramethylene glycol) and crosslinked by trimethylol propane (TMP). The two fillers investigated were barium sulphate and Expancel which were used to dissipate and scatter sonic waves in acoustic damping applications. [Continues.

Gene Programs of Spinal Cord Cells Mediating Pain States: Identification of Protein targeting to glycogen as an induced gene in spinal cord astrocytes upon peripheral pain

Nociceptive pain signals are relayed in the spinal cord as they are transmitted from the periphery to higher brain centers. The neuronal populations in the spinal cord that transmit peripheral stimuli are very heterogeneous. This has hampered the identification of interneuron subtypes involved in pain processing and the characterization of their functional connectivities. In this study, I obtained a transcriptomic profile of spinal cord cells specifically activated by a peripheral painful stimulus using the recently developed phospho-ribosome profiling technique, and identified Protein targeting to glycogen (Ptg) as a pain-induced gene in spinal astrocytes. Ptg is known to play an important role in glycogenesis. I observed elevated spinal cord glycogen levels in response to different painful stimuli and proposed a correlation between the magnitude and duration of glycogen elevation with the persistency of different pain models. Moreover, manipulation of Ptg expression and glycogen metabolism led to altered pain sensitivity. My study points toward a new perspective of the role of astrocytes in pain processing and a potential link between changes in the metabolic state and pain processing in the spinal cord

Accelerated structural evolution of galaxies in a starbursting cluster at z=2.51

Structural properties of cluster galaxies during their peak formation epoch, $z \sim 2-4$ provide key information on whether and how environment affects galaxy formation and evolution. Based on deep HST/WFC3 imaging towards the z=2.51 cluster, J1001, we explore environmental effects on the structure, color gradients, and stellar populations of a statistical sample of cluster SFGs. We find that the cluster SFGs are on average smaller than their field counterparts. This difference is most pronounced at the high-mass end ($M_{\star} > 10^{10.5} M_{\odot}$) with nearly all of them lying below the mass-size relation of field galaxies. The high-mass cluster SFGs are also generally old with a steep negative color gradient, indicating an early formation time likely associated with strong dissipative collapse. For low-mass cluster SFGs, we unveil a population of compact galaxies with steep positive color gradients that are not seen in the field. This suggests that the low-mass compact cluster SFGs may have already experienced strong environmental effects, e.g., tidal/ram pressure stripping, in this young cluster. These results provide evidence on the environmental effects at work in the earliest formed clusters with different roles in the formation of low and high-mass galaxies.Comment: 13 pages, 10 figures, 1 tabl

PRIMA-1Met suppresses colorectal cancer independent of p53 by targeting MEK

This work was supported by Grant No. 81201779 (Hua Xiong) from the National Natural Science Youth Foundation; Grant No. 81502118 (Yanmei Zou) from the National Natural Science Youth Foundation; Grant No. 2014CFB250 (Yanmei Zou) from the Natural Science Foundation of Hubei Province; Grant No. 81372434 (Huihua Xiong) from the National Natural Science Foundation.PRIMA-1Met is the methylated PRIMA-1 (p53 reactivation and induction of massive apoptosis) and could restore tumor suppressor function of mutant p53 and induce p53 dependent apoptosis in cancer cells harboring mutant p53. However, p53 independent activity of PRIMA-1Met remains elusive. Here we reported that PRIMA-1Met attenuated colorectal cancer cell growth irrespective of p53 status. Kinase profiling revealed that mitogen-activated or extracellular signal-related protein kinase (MEK) might be a potential target of PRIMA-1Met. Pull-down binding and ATP competitive assay showed that PRIMA-1Met directly bound MEK in vitro and in cells. Furthermore, the direct binding sites of PRIMA-1Met were explored by using a computational docking model. Treatment of colorectal cancer cells with PRIMA-1Met inhibited p53-independent phosphorylation of MEK, which in turn impaired anchorage-independent cell growth in vitro. Moreover, PRIMA-1Met suppressed colorectal cancer growth in xenograft mouse model by inhibiting MEK1 activity. Taken together, our findings demonstrate a novel p53-independent activity of PRIMA-1Met to inhibit MEK and suppress colorectal cancer growth.Publisher PDFPeer reviewe

Developing and validating a nomogram for cognitive impairment in the older people based on the NHANES

ObjectiveTo use the United States National Health and Nutrition Examination Study (NHANES) to develop and validate a risk-prediction nomogram for cognitive impairment in people aged over 60 years.MethodsA total of 2,802 participants (aged ≥ 60 years) from NHANES were analyzed. The least absolute shrinkage and selection operator (LASSO) regression model and multivariable logistic regression analysis were used for variable selection and model development. ROC-AUC, calibration curve, and decision curve analysis (DCA) were used to evaluate the nomogram’s performance.ResultsThe nomogram included five predictors, namely sex, moderate activity, taste problem, age, and education. It demonstrated satisfying discrimination with a AUC of 0.744 (95% confidence interval, 0.696–0.791). The nomogram was well-calibrated according to the calibration curve. The DCA demonstrated that the nomogram was clinically useful.ConclusionThe risk-prediction nomogram for cognitive impairment in people aged over 60 years was effective. All predictors included in this nomogram can be easily accessed from its’ user

Resident Immune Cells of the Liver in the Tumor Microenvironment

The liver is a central immunomodulator that ensures a homeostatic balance between protection and immunotolerance. A hallmark of hepatocellular carcinoma (HCC) is the deregulation of this tightly controlled immunological network. Immune response in the liver involves a complex interplay between resident innate, innate, and adaptive immune cells. The immune response in the liver is modulated by its continuous exposure to toxic molecules and microorganisms that requires a degree of immune tolerance to protect normal tissue from damage. In HCC pathogenesis, immune cells must balance a dual role that includes the elimination of malignant cells, as well as the repair of damaged liver tissue to maintain homeostasis. Immune response in the innate and adaptive immune systems extends to the cross-talk and interaction involving immune-regulating non-hematopoietic cells, myeloid immune cells, and lymphoid immune cells. In this review, we discuss the different immune responses of resident immune cells in the tumor microenvironment. Current FDA-approved targeted therapies, including immunotherapy options, have produced modest results to date for the treatment of advanced HCC. Although immunotherapy therapy to date has demonstrated its potential efficacy, immune cell pathways need to be better understood. In this review article, we summarize the roles of specific resident immune cell subsets and their cross-talk subversion in HCC pathogenesis, with a view to identifying potential new biomarkers and therapy options

Isolation and characterization of the mink interferon-epsilon gene and its antiviral activity

The interferon (IFN) response is the first line of defense against viral invasion and thus plays a central role in the regulation of the immune response. IFN-epsilon (IFN-ε) is a newly discovered type I IFN that does not require viral induction, unlike other type I IFNs. IFN-ε is constitutively expressed in epithelial cells and plays an important role in mucosal immunity. In this study, we evaluated the biological activity of the mink-IFN (MiIFN)-ε gene in prokaryotic cells. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate IFN-ε expression in different mink tissues. MiIFN-ε was highly expressed in brain, lung, tracheal, kidney, intestinal, bladder, ovarian, and testis tissues. There was no significant difference in MiIFN-ε expression between female and male minks, except in the reproductive system. Expression of the small ubiquitin-like modifier (SUMO3)-MiIFN-ε fusion gene was induced by isopropylβ-d-thiogalactoside, and MiIFN-ε was collected after SUMO-specific protease digestion. We tested the antiviral activity of MiIFN-ε against vesicular stomatitis virus (VSV) in epithelial cells of feline kidney 81 (F81). We used qRT-PCR to analyze the expression of several IFN-stimulated genes (ISGs), including ISG15, 2′-5′ oligoadenylate synthetase (2′-5′OAS1), and myxovirus resistance protein 1 (Mx1). Recombinant IFN-ε induced high ISG expression in F81 cells. Compared with those in the cell control group, expressions of ISG15, Mx1, and 2′-5′ OAS1 in the VSV-GFP control, IFN-ε, and MiIFN-ε-inhibited VSV-GFP groups were significantly increased. Compared with those in the VSV-GFP control group, expressions of ISG15 and 2′-5′ OAS1 in the IFN-ε and MiIFN-ε-inhibited VSV-GFP groups were significantly increased, and the differences were highly significant (p < 0.0001). IFN-ε played an indirect antiviral role. These findings lay the foundation for detailed investigation of IFN-ε in the future