Pengaruh Pemberian Terapi Minyak Nigella Sativa Dan Kombinasinya Dengan Seftriakson Terhadap Jumlah Kuman Methicillin Resistant Staphylococcus Aureus (Mrsa) Pada Kultur Limpa Mencit Balb/c

Background: Levels of serum albumin can be used as a predictor of morbidity and mortality in the elderly. Reduced serum albumin concentration can be caused by oxidative modification due to aging or insufficient protein intake. SOD as an enzymatic antioxidant might prevent oxidative stress so that albumin modification process can be inhibited. SOD supplementation was expected to increase serum albumin levels.Aim: To prove the effect of Nigella sativa oil and its combination with ceftriaxone toward MRSA count in BALB/c mice\u27s spleen culture.Methods: This was true laboratory experimental study with post test only control group design. The samples were 20 males BALB/c mice, randomized into 4 groups. Control was given 0,03 ml aquabidest injected intraperitoneally, P1 was given 0,03 ml ceftriaxone injected intraperitoneally, P2 was given 0,3 ml Nigella sativa oil orally and P3 was given the combination of 0,03 ml ceftriaxone and 0,3 ml Nigella sativa oil. 0,2 ml (107 cfu/ml) MRSA was injected intraperitoneally at the hour 0. The treatment was given at the hour 16 and mice were terminated at the hour 24. Kruskall-Wallis Test and Mann-Whitney Test is used to analyze the data.Results: Results of the mean number of MRSA bacteria in spleen culture were K=124x103 160,70x103; P1=24,08x103 26,53x103; P2=0,78x103 1,52x103; P3=6,05x103 13,38x103. The number of MRSA bacteria decreased significantly between control group compared to P2 (p=0,016) and P3 (p=0,046) and P1 compared to P2 (p=0,016) and P3 (p=0,047). There were no significance between control compared to P1 (p=0,674) and P2 toward P3 (p=0,596).Conclusion: Administration of Nigella sativa oil and its combination with ceftriaxone can decrease the number of MRSA bacteria in BALB/c mice\u27s spleen cultures

Additional file 8: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Time-lapse recording for microtubular organization as revealed by VAEM. (AVI 1072 kb

Additional file 6: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Dual-color VAEM indicates clathrin light chain (CLC)–GFP and mCherry-Flot1a showing characteristic localization to separated punctate structures. (AVI 127 kb

Additional file 7: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Rapid turnover of actin filaments in transgenic fABD2-GFP Arabidopsis seedlings in the proximity of plasma membrane. (AVI 1151 kb

Additional file 4: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Dynamics of mitochondria in the proximity of plasma membrane. (AVI 23044 kb

Additional file 2: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Figure S1. Organellar dynamics are resolved in high spatio-temporal manner under VAEM. Figure S2. VAEM is applicable to epidermal cells of different tissues from seedlings. Figure S3. CLC-GFP tagged punctate structures correspond to clathrin-coated vesicles and Golgi apparatuses, depending on the angle of incident light. Figure S4. Actin turnover resolved using VAEM. Figure S5. Microtubular organization as revealed by VAEM. Figure S6. SKU5-GFP localizes to plasma membrane and intracellular structures. Figure S7. Distribution of the fluorescence intensity of the diffraction-limited single pCLC2- myristoyl-mGFPA206K spots. Figure S8. Fluorescence correlation spectroscopy (FCS) to examine the fluorescence fluctuation in response to TyrA23 treatment. Figure S9. Analysis on MSD for different trajectories and categorization into various diffusion regimes. Figure S10. Representative kymographs for BOR1 and Lti6a. (PDF 1148 kb

Additional file 1: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Methodology for determining incident angle and penetration depth. (PDF 569  kb

Additional file 3: of Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells

Dynamics of Golgi apparatuses in the proximity of plasma membrane. (AVI 23044 kb

Oxidative Stress Acts on Special Membrane Proteins To Reduce the Viability of <i>Pseudomonas syringae</i> pv <i>tomato</i>

Reactive oxygen species (ROS) play a vital role in reducing the viability of invading pathogens during plant-pathogen interactions. To understand how oxidative stress caused by ROS reduces cell viability, it is important to identify the proteins affected by ROS. In the present study, we investigated the changes in the expression of proteins from the outer and inner membrane fractions in <i>Pseudomonas syringae</i> pv <i>tomato</i> DC3000 under oxidative stress through membrane subproteomics. A total number of 17 differentially expressed proteins from the outer and inner membrane fractions were identified, among which 11 proteins belong to transporters, such as porins and ABC transporters. Their abundance was all decreased under oxidative stress, indicating that transporters are likely to be affected by oxidative stress. The function of two identified transporters was further characterized by constructing their gene mutant and overexpression strains. We found that mutation of one transporter gene <i>PSPTO_1720</i> rendered <i>Pseudomonas</i> more sensitive to oxidative stress, whereas overexpression of this gene made the strain more resistant. By comparison, the mutant and overexpression strains of another transporter gene <i>PSPTO_2152</i> exhibited the same sensitivity to oxidative stress compared with the wild-type. Our data suggest that oxidative stress reduces the viability of bacterial cells by acting on special transporters

Additional file 9: Table S8. of Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3

Identification of proteins that interact with SlVPE3 using SWATH-MS. (XLS 168 kb