The Role of Rumination and Negative Affect in Meaning Making Following Stressful Experiences in a Japanese Sample

Stressful experiences can lead to meaning making that is seen as central in adjustment. Although rumination and negative affect are important factors of meaning making, little is known about the mechanisms involved. This study aimed to examine the meaning making process, focusing on the role of intrusive and deliberate rumination and negative affect. The principal hypotheses were as follows: negative affect is positively related to threat evaluation and intrusive rumination, while regret and guilt are positively related to deliberate rumination; intrusive rumination is negatively related to finding meaning, whereas deliberate rumination is positively related to finding meaning. A total of 383 undergraduate students were asked to remember their most stressful life event and complete a questionnaire containing the Event Related Rumination Inventory and items about negative affect, threat evaluation, and finding meaning about the stressful life event. For 342 of the final sample, structural equation modeling based on the study hypotheses showed that both deliberate and intrusive rumination immediately after the event were positively associated with finding meaning. Intrusive rumination at present, however, was negatively related to finding meaning. This study also revealed the effects of negative affect: helplessness, sadness, and fear induced intrusive rumination; moreover, regret was positively associated with deliberate rumination

Emotion Regulation and emotional experiences : An examination based on individual differences in terms of cultural self-construal

We examined the hypothesis that cognitive reappraisal is ineffective for people who have either a low sense of independence or a high sense of interdependence. We also examined whether culturalself-construal is related to the use of emotion regulation strategies and whether such use leads to emotional experiences in daily life. The respondents were 314 students, who completed scales aessing cultural self-construal (independence and interdependence)，use of cognitive reappraisal and suppression，and daily emotional experiences. The results indicate that the use of cognitive reappraisalis negatively related to daily experiences of negative emotions only for interindependent individuals. Furthermore，there is support for a model that includes both processes where cultural self-construal is directly associated with emotional experiences and processes that are mediated by the use of emotion regulation strategies. These findings suggest two possibilities; the first is that daily use of cognitive reappraisal is effective regardless of cultural self-construal and the second is that the relations between cultural self-construal and emotional experiences can be partly explained by the use of emotion regulation strategies

The Role of Rumination and Negative Affect in Meaning Making Following Stressful Experiences in a Japanese Sample

Stressful experiences can lead to meaning making that is seen as central in adjustment. Although rumination and negative affect are important factors of meaning making, little is known about the mechanisms involved. This study aimed to examine the meaning making process, focusing on the role of intrusive and deliberate rumination and negative affect. The principal hypotheses were as follows: negative affect is positively related to threat evaluation and intrusive rumination, while regret and guilt are positively related to deliberate rumination; intrusive rumination is negatively related to finding meaning, whereas deliberate rumination is positively related to finding meaning. A total of 383 undergraduate students were asked to remember their most stressful life event and complete a questionnaire containing the Event Related Rumination Inventory and items about negative affect, threat evaluation, and finding meaning about the stressful life event. For 342 of the final sample, structural equation modeling based on the study hypotheses showed that both deliberate and intrusive rumination immediately after the event were positively associated with finding meaning. Intrusive rumination at present, however, was negatively related to finding meaning. This study also revealed the effects of negative affect: helplessness, sadness, and fear induced intrusive rumination; moreover, regret was positively associated with deliberate rumination

Current Immunotherapeutic Approaches in Pancreatic Cancer

Pancreatic cancer is a highly aggressive and notoriously difficult to treat. As the vast majority of patients are diagnosed at advanced stage of the disease, only a small population is curative by surgical resection. Although gemcitabine-based chemotherapy is typically offered as standard of care, most patients do not survive longer than 6 months. Thus, new therapeutic approaches are needed. Pancreatic cancer cells that develop gemcitabine resistance would still be suitable targets for immunotherapy. Therefore, one promising treatment approach may be immunotherapy that is designed to target pancreatic-cancer-associated antigens. In this paper, we detail recent work in immunotherapy and the advances in concept of combination therapy of immunotherapy and chemotherapy. We offer our perspective on how to increase the clinical efficacy of immunotherapies for pancreatic cancer

A novel ex vivo lung cancer model based on bioengineered rat lungs

Introduction: Two-dimensional cell cultures have contributed substantially to lung cancer research, but 3D cultures are gaining attention as a new, more efficient, and effective research model. A model reproducing the 3D characteristics and tumor microenvironment of the lungs in vivo, including the co-existence of healthy alveolar cells with lung cancer cells, is ideal. Here, we describe the creation of a successful ex vivo lung cancer model based on bioengineered lungs formed by decellularization and recellularization.Methods: Human cancer cells were directly implanted into a bioengineered rat lung, which was created with a decellularized rat lung scaffold reseeded with epithelial cells, endothelial cells and adipose-derived stem cells. Four human lung cancer cell lines (A549, PC-9, H1299, and PC-6) were applied to demonstrate forming cancer nodules on recellularized lungs and histopathological assessment were made among these models. MUC-1 expression analysis, RNA-seq analysis and drug response test were performed to demonstrate the superiority of this cancer model.Results: The morphology and MUC-1 expression of the model were like those of lung cancer in vivo. RNA sequencing revealed an elevated expression of genes related to epithelial-mesenchymal transition, hypoxia, and TNF-α signaling via NF-κB; but suppression of cell cycle-related genes including E2F. Drug response assays showed that gefitinib suppressed PC-9 cell proliferation equally well in the 3D lung cancer model as in 2D culture dishes, albeit over a smaller volume of cells, suggesting that fluctuations in gefitinib resistance genes such as JUN may affect drug sensitivity.Conclusions: A novel ex vivo lung cancer model was closely reproduced the 3D structure and microenvironment of the actual lungs, highlighting its possible use as a platform for lung cancer research and pathophysiological studies

Mind wandering in creative problem-solving: Relationships with divergent thinking and mental health.

Previous research has shown that mind wandering has both positive and negative effects. Mind wandering may improve creative problem solving; however, it could also lead to negative moods and poor mental health. It has also been shown that some forms of mental illness are positively related to creativity. However, the three factors of mind wandering, divergent thinking, and mental health have not been examined simultaneously, so it is possible that these relationships are manifested by spurious correlations. Therefore, we examined the relations among the three factors while controlling for each of their confounding effects. We asked 865 participants (458 men, 390 women, 17 unknown; Mage = 18.99 years, SD = 1.16) to complete a questionnaire measuring mind wandering traits, divergent thinking, and mental health measures including depressive symptoms and schizotypal personality. Multiple regression analysis showed that people who reported more depressive symptoms, schizotypal personality, and divergent thinking, were more likely to engage in mind-wandering. Our results indicated that frequency of mind wandering was linked to a risk of poorer mental health as well as to higher divergent thinking ability. In future research, we will examine the features of mind wandering related to divergent thinking and mental health by considering the contents of wandering thoughts and whether they are ruminative or not. We also need to examine whether the same results will be found when studying professionals in creative occupations, and when using different scoring methods in divergent thinking tests