354 research outputs found
NEIL1 (nei endonuclease VIII-like 1 (E. coli))
Review on NEIL1 (nei endonuclease VIII-like 1 (E. coli)), with data on DNA, on the protein encoded, and where the gene is implicated
Molecular cloning of the new forth allotype of the factor H in the Russian and Kazakhstan wild mouse population
Variational calculations of the -seperation energy of the O hypernucleus
Variational Monte Carlo calculations have been made for the O hypernucleus using realistic two- and three-baryon
interactions. A two pion exchange potential with spin- and space-exchange
components is used for the N potential. Three-body two-pion exchange
and strongly repulsive dispersive NN interactions are also included.
The trial wave function is constructed from pair- and triplet-correlation
operators acting on a single particle determinant. These operators consist of
central, spin, isospin, tensor and three- baryon potential components. A
cluster Monte Carlo method is developed for noncentral correlations and is used
with up to four-baryon clusters in our calculations. The three-baryon
NN force is discussed.Comment: 24 pages, 2 figs available by fax., for publication in Phys. Rev.
Phenomenological Lambda-Nuclear Interactions
Variational Monte Carlo calculations for (ground and
excited states) and are performed to decipher information on
-nuclear interactions. Appropriate operatorial nuclear and
-nuclear correlations have been incorporated to minimize the
expectation values of the energies. We use the Argonne two-body
NN along with the Urbana IX three-body NNN interactions. The study demonstrates
that a large part of the splitting energy in () is
due to the three-body NN forces. hypernucleus is
analyzed using the {\it s}-shell results. binding to nuclear matter
is calculated within the variational framework using the
Fermi-Hypernetted-Chain technique. There is a need to correctly incorporate the
three-body NN correlations for binding to nuclear matter.Comment: 18 pages (TeX), 2 figure
A Realistic Description of Nucleon-Nucleon and Hyperon-Nucleon Interactions in the SU_6 Quark Model
We upgrade a SU_6 quark-model description for the nucleon-nucleon and
hyperon-nucleon interactions by improving the effective meson-exchange
potentials acting between quarks. For the scalar- and vector-meson exchanges,
the momentum-dependent higher-order term is incorporated to reduce the
attractive effect of the central interaction at higher energies. The
single-particle potentials of the nucleon and Lambda, predicted by the G-matrix
calculation, now have proper repulsive behavior in the momentum region q_1=5 -
20 fm^-1. A moderate contribution of the spin-orbit interaction from the
scalar-meson exchange is also included. As to the vector mesons, a dominant
contribution is the quadratic spin-orbit force generated from the rho-meson
exchange. The nucleon-nucleon phase shifts at the non-relativistic energies up
to T_lab=350 MeV are greatly improved especially for the 3E states. The
low-energy observables of the nucleon-nucleon and the hyperon-nucleon
interactions are also reexamined. The isospin symmetry breaking and the Coulomb
effect are properly incorporated in the particle basis. The essential feature
of the Lambda N - Sigma N coupling is qualitatively similar to that obtained
from the previous models. The nuclear saturation properties and the
single-particle potentials of the nucleon, Lambda and Sigma are reexamined
through the G-matrix calculation. The single-particle potential of the Sigma
hyperon is weakly repulsive in symmetric nuclear matter. The single-particle
spin-orbit strength for the Lambda particle is very small, in comparison with
that of the nucleons, due to the strong antisymmetric spin-orbit force
generated from the Fermi-Breit interaction.Comment: Revtex v2.09, 69 pages with 25 figure
Predicting the demand of physician workforce: an international model based on "crowd behaviors"
<p>Abstract</p> <p>Background</p> <p>Appropriateness of physician workforce greatly influences the quality of healthcare. When facing the crisis of physician shortages, the correction of manpower always takes an extended time period, and both the public and health personnel suffer. To calculate an appropriate number of Physician Density (PD) for a specific country, this study was designed to create a PD prediction model, based on health-related data from many countries.</p> <p>Methods</p> <p>Twelve factors that could possibly impact physicians' demand were chosen, and data of these factors from 130 countries (by reviewing 195) were extracted. Multiple stepwise-linear regression was used to derive the PD prediction model, and a split-sample cross-validation procedure was performed to evaluate the generalizability of the results.</p> <p>Results</p> <p>Using data from 130 countries, with the consideration of the correlation between variables, and preventing multi-collinearity, seven out of the 12 predictor variables were selected for entry into the stepwise regression procedure. The final model was: PD = (5.014 - 0.128 × proportion under age 15 years + 0.034 × life expectancy)<sup>2</sup>, with R<sup>2 </sup>of 80.4%. Using the prediction equation, 70 countries had PDs with "negative discrepancy", while 58 had PDs with "positive discrepancy".</p> <p>Conclusion</p> <p>This study provided a regression-based PD model to calculate a "norm" number of PD for a specific country. A large PD discrepancy in a country indicates the needs to examine physician's workloads and their well-being, the effectiveness/efficiency of medical care, the promotion of population health and the team resource management.</p
Evidence for the Involvement of DNA Repair Enzyme NEIL1 in Nucleotide Excision Repair of (5′R)- and (5′S)-8,5′-Cyclo-2′-deoxyadenosines†
Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology
Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next- generation sequencing on 56 samples of widespread man- grove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range
A haplotype variation affecting the mitochondrial transportation of hMYH protein could be a risk factor for colorectal cancer in Chinese
<p>Abstract</p> <p>Background</p> <p>The human MutY homolog (<it>hMYH</it>), a DNA glycolsylase involved in the excision repair of oxidative DNA damage, is currently studied in colorectal cancer (CRC). We previously demonstrated a haplotype variant c.53C>T/c.74G>A of <it>hMYH </it>(T/A) increasing the risk for gastric cancer in Chinese. However, most investigations on correlation between <it>hMYH </it>and CRC are conducted in Western countries and the underlying mechanism has been poorly understood.</p> <p>Methods</p> <p>To determine whether the haplotype T/A variant of <it>hMYH </it>was related to colorectal carcinogenesis, we performed a case-control study in 138 colorectal cancer (CRC) patients and 343 healthy controls in a Chinese population. Furthermore, the C/G for wild-type, C/A or T/G for single base variant and T/A for haplotype variant <it>hMYH </it>cDNAs with a flag epitope tag were cloned into pcDNA3.1+ vector and transfected into cos-7 cell line. Their subcellular localizations were determined by immunofluorescence assay.</p> <p>Results</p> <p>It was found that the frequency of haplotype variant allele was statistically higher in CRC patients than that in controls (<it>P </it>= 0.02, odds ratio = 5.06, 95% confidence interval = 1.26 – 20.4). Similarly, significant difference of heterozygote frequency was indicated between the two groups (<it>P </it>= 0.019), while no homozygote was found. In addition, immunofluorescence analysis showed that hMYH protein with haplotype T/A variation presented in both nucleus and mitochondria, in contrast to the wild-type protein only converging in mitochondria. However, neither of the single missense mutations alone changed the protein subcelluar localization.</p> <p>Conclusion</p> <p>Although preliminarily, these results suggest that: the haplotype variant allele of <it>hMYH </it>leads to a missense protein, which partly affects the protein mitochondrial transportation and results as nuclear localization. This observation might be responsible for the increased susceptibility to cancers, including CRC, in Chinese.</p
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