41 research outputs found
Abundance of the Major Chloroplast Polypeptides during Development and Ripening of Tomato Fruits
A compatible interaction of Alternaria brassicicola with Arabidopsis thaliana ecotype DiG: evidence for a specific transcriptional signature
<p>Abstract</p> <p>Background</p> <p>The interaction of <it>Arabidopsis </it>with <it>Alternaria brassicicola </it>provides a model for disease caused by necrotrophs, but a drawback has been the lack of a compatible pathosystem. Infection of most ecotypes, including the widely-studied line Col-0, with this pathogen generally leads to a lesion that does not expand beyond the inoculated area. This study examines an ecotype, Dijon G (DiG), which is considered sensitive to <it>A. brassicicola</it>.</p> <p>Results</p> <p>We show that the interaction has the characteristics of a compatible one, with expanding rather than limited lesions. To ask whether DiG is merely more sensitive to the pathogen or, rather, interacts in distinct manner, we identified genes whose regulation differs between Col-0 and DiG challenged with <it>A. brassicicola</it>. Suppression subtractive hybridization was used to identify differentially expressed genes, and their expression was verified using semi-quantitative PCR. We also tested a set of known defense-related genes for differential regulation in the two plant-pathogen interactions. Several known pathogenesis-related (<it>PR</it>) genes are up-regulated in both interactions. <it>PR1</it>, and a monooxygenase gene identified in this study, <it>MO1</it>, are preferentially up-regulated in the compatible interaction. In contrast, <it>GLIP1</it>, which encodes a secreted lipase, and <it>DIOX1</it>, a pathogen-response related dioxygenase, are preferentially up-regulated in the incompatible interaction.</p> <p>Conclusion</p> <p>The results show that DiG is not only more susceptible, but demonstrate that its interaction with <it>A. brassicicola </it>has a specific transcriptional signature.</p
Differential Changes in the Amount of Protein Complexes in the Chloroplast Membrane during Senescence of Oat and Bean Leaves
Branched-chain and aromatic amino acid catabolism into aroma volatiles in Cucumis melo L. fruit
The unique aroma of melons (Cucumis melo L., Cucurbitaceae) is composed of many volatile compounds biosynthetically derived from fatty acids, carotenoids, amino acids, and terpenes. Although amino acids are known precursors of aroma compounds in the plant kingdom, the initial steps in the catabolism of amino acids into aroma volatiles have received little attention. Incubation of melon fruit cubes with amino acids and Ī±-keto acids led to the enhanced formation of aroma compounds bearing the side chain of the exogenous amino or keto acid supplied. Moreover, L-[13C6]phenylalanine was also incorporated into aromatic volatile compounds. Amino acid transaminase activities extracted from the flesh of mature melon fruits converted L-isoleucine, L-leucine, L-valine, L-methionine, or L-phenylalanine into their respective Ī±-keto acids, utilizing Ī±-ketoglutarate as the amine acceptor. Two novel genes were isolated and characterized (CmArAT1 and CmBCAT1) encoding 45.6ākDa and 42.7ākDa proteins, respectively, that displayed aromatic and branched-chain amino acid transaminase activities, respectively, when expressed in Escherichia coli. The expression of CmBCAT1 and CmArAT1 was low in vegetative tissues, but increased in flesh and rind tissues during fruit ripening. In addition, ripe fruits of climacteric aromatic cultivars generally showed high expression of CmBCAT1 and CmArAT1 in contrast to non-climacteric non-aromatic fruits. The results presented here indicate that in melon fruit tissues, the catabolism of amino acids into aroma volatiles can initiate through a transamination mechanism, rather than decarboxylation or direct aldehyde synthesis, as has been demonstrated in other plants