<ORIGINAL ARTICLE>Localization of anti-monocyte/macrophage antibody-positive cells in periodontal tissue of rat maxillary molars after orthodontic tooth movement

To examine the localization of monoclonal anti-monocyte/macrophage (ED1) and macrophage (ED2) antibody-positive cells in periodontium, rat maxillary molar teeth were moved by insertion of band materials. The orthodontic tooth movement was elicited for 5 days, and paraffin-embedded maxillary teeth were stained by fluorescent immunocytochemistry and observed using a confocal laser scanning microscope. The localization of ED1-positive mononuclear cells in the experimental teeth was little different from that in the controls. While ED2-positive mononuclear cells were located throughout the periodontium on the distalside of controls, the number of positive cells decreased on the pressure side of the treated teeth. The present study suggested that most of the immunoreactive mononuclear cells on the distal side of controls are macrophages, while the positive cells on the pressure side of the experimental teeth are osteoclast precursors and a small number of macrophages

<ORIGINAL ARTICLE>The effects of sagittal ramus osteotomy for mandibular prognathism on maximum mouth opening and condylar movement

Maximum mouth opening and condylar movement before and more than 6 months after surgery were analyzed in 23 cases of sagittal ramus osteotomy of the mandible for correction of mandibular prognathism. Condylar movement (translation and rotation) did not show postoperatively a significant difference pre-and postoperatively, and then was a tendency to a reduction of maximum mouth opening was found

Development of a safe and quick method for removal of intermaxillary fixation with superelastic Ni-Ti wire

To improve emergency intermaxillary fixation release, a novel method of intermaxillary fixation, in which super-elastic nickel-titan (Ni-Ti) alloy wires were applied at 2 places (Method A) was developed. Method A was compared to the previous method (Method B : fixing the jaw at 3 places with stainless steel wires), in terms of the time required to remove the wires and the number of pieces of cut wire left in the oral cavity and pharynx. The average time for removing the wires was 14.5 ± 9.9 (mean ± SD) seconds for Method A, and 79.1 ± 53.1 seconds for Method B. The average time was significantly shorter in Method A than in Method B (p < 0.01). The number of pieces of cut wire left was zero with Method A. These findings suggest that the novel method (Method A) provides quick and safe wire removal and improves the safety and quality of dentistry in emergency cases

Short report: possible Cryptosporidium muris infection in humans.

Oocysts of cryptosporidia whose morphology resembled that of Cryptosporidium muris were found in the stool of 2 healthy girls in Surabaya, Indonesia. The oocysts were predominantly oval and measured 7.75+/-0.17 x 5.55+/-0.13 microm (mean+/-SD). The number of oocysts excreted were more than 10(5) per gram of stool. The oocysts were well stained with fluorescein-conjugated monoclonal antibody to Cryptosporidium. The specimens from both girls containing the oocysts showed a positive result by the polymerase chain reaction (PCR) using primers specific for the genus Cryptosporidium, but a negative result by the PCR using primers specific for C. parvum. The 2 girls passed oocysts for 5 and 6 days, respectively. They did not complain of any symptoms during the passage of oocysts

Short report: possible Cryptosporidium muris infection in humans.

Oocysts of cryptosporidia whose morphology resembled that of Cryptosporidium muris were found in the stool of 2 healthy girls in Surabaya, Indonesia. The oocysts were predominantly oval and measured 7.75+/-0.17 x 5.55+/-0.13 microm (mean+/-SD). The number of oocysts excreted were more than 10(5) per gram of stool. The oocysts were well stained with fluorescein-conjugated monoclonal antibody to Cryptosporidium. The specimens from both girls containing the oocysts showed a positive result by the polymerase chain reaction (PCR) using primers specific for the genus Cryptosporidium, but a negative result by the PCR using primers specific for C. parvum. The 2 girls passed oocysts for 5 and 6 days, respectively. They did not complain of any symptoms during the passage of oocysts