Clinicopathological and targeted exome gene features of a patient with metastatic acinic cell carcinoma of the parotid gland harboring an ARID2 nonsense mutation and CDKN2A/B deletion

We describe the presentation, treatment, clinical outcome, and targeted genome analysis of a metastatic salivary acinic cell carcinoma (AciCC). A 71-year-old male presented with a 3 cm right tail of a parotid lesion, first detected as a nodule by the patient seven months earlier. He had a right total parotidectomy with cranial nerve VII resection, right facial nerve resection and grafting, resection of the right conchal cartilage, and right modified radical neck dissection. The primary tumor revealed AciCC with two distinct areas: a well-differentiated component with glandular architecture and a dedifferentiated component with infiltrative growth pattern associated with prominent stromal response, necrosis, perineural invasion, and cellular pleomorphism. Tumor staging was pT4 N0 MX. Immunohistochemistry staining showed pankeratin (+), CD56 (−), and a Ki67 proliferation index of 15%. Upon microscopic inspection, 49 local lymph nodes resected during parotidectomy were negative for cancer cells. Targeted sequencing of the primary tumor revealed deletions of CDKN2A and CDKN2B, a nonsense mutation in ARID2, and single missense mutations of unknown significance in nine other genes. Despite postoperative localized radiation treatment, follow-up whole body PET/CT scan showed lung, soft tissue, bone, and liver metastases. The patient expired 9 months after resection of the primary tumor

Burden of Disease in Japan: Using National and Subnational Data to Inform Local Health Policy

The Global Burden of Disease (GBD) study has been instrumental in guiding global health policy development since the early 1990s. The GBD 2010 project provided rich information about the key causes of mortality, disability-adjusted life years, and their associated risk factors in Japan and provided a unique opportunity to incorporate these data into health planning. As part of the latest update of this project, GBD 2013, the Japanese GBD collaborators plan to update and refine the available burden of disease data by incorporating sub-national estimates of the burden of disease at the prefectural level. These estimates will provide health planners and policy makers at both the national and prefectural level with new, more refined tools to adapt local public health initiatives to meet the health needs of local populations. Moreover, they will enable the Japanese health system to better respond to the unique challenges in their rapidly aging population and as a complex combination of non-communicable disease risk factors begin to dominate the policy agenda. Regional collaborations will enable nations to learn from the experiences of other nations that may be at different stages of the epidemiological transition and have different exposure profiles and associated health effects. Such analyses and improvements in the data collection systems will further improve the health of the Japanese, maintain Japan's excellent record of health equity, and provide a better understanding of the direction of health policy in the region

Lack of Methylene Blue Staining in Superficial Epithelia as a Possible Marker for Superficial Lateral Spread of Bile Duct Cancer

Longitudinal cancer spread is very important for staging of resectability in bile duct cancer. We verified the difference in methylene blue staining properties between cancerous and noncancerous epithelia that are usually observed by cholangioscopy. We obtained 45 biopsy specimens from the common bile duct of 20 patients with bile duct disease using percutaneous transhepatic cholangioscopy (PTCS) after staining with 0.05% methylene blue. We compared the microscopic staining properties with the gross endoscopic observations and evaluated the characteristics of methylene blue staining on frozen sections of each type of cholangial epithelium. Microscopic staining properties were significantly associated with endoscopic observations (p = 0.00001). While 18 of 20 (90%) specimens of normal epithelia stained with methylene blue, 11 of 16 (69%) specimens of metaplastic epithelia were stained, with no staining obtained in cancerous epithelia. The cancerous epithelia stained significantly less often than either the normal (p = 0.000005) or the metaplastic (p = 0.001) epithelia. Evaluation of methylene blue staining during PTCS revealed that this stain was absorbed by the cholangial epithelia, not superficially stuck to it. The difference in methylene blue staining properties between the cancerous and normal epithelia could be helpful to clarify the boundary of superficial lateral spread of bile duct cancer

A Case Study of Assessing Button Bits Failure through Wavelet Transform Using Rock Drilling Induced Noise Signals

Finding the precise moment of button breakage of bits during drilling, with the experience of drill rig operators is a serious concern for modern vibrant mining industry. This research proposed a new methodology to find the failure of button using the sound generated by rock-bit interactions. The experiment is conducted by the video and sound data recorded during a drilling process in an underground mine, that uses a Sandvik AXERA7 twin boom jumbo drill rig and Polycrystalline diamond (PCD) tapered button bits. Signal analysis techniques such as Fourier transform and Wavelet transform are utilised to analyse the hectic noise signal recorded. The analysed results are shown that Wavelet Transform is much more effective in finding singularity points such as chipping or breakage of a button in compared to the Fourier Transform. The outcome of this analysis, which is the peak intensity at the breakage point, was correlated to the average intensity of the sound wave using moving average method. The results suggest that the noise generated during the drilling process can be used to detect the condition of the drill bit

Symmetry-adapted HAM/3 method and its application to some symmetric molecules

The semiempirical HAM/3 method developed by Lindholm and coworkers about two decades ago has been known to have a deficiency that splits energies for the degenerate energy states. We have recently proposed a group-theoretical approach to remedy the internally broken symmetry of the HAM/3 Hamiltonians. In this paper, we present some results of its application to various small molecules with symmetry Td, C3v, and D3h. The proposed scheme gives correct degeneracy for these molecules.O método semi-empírico HAM/3, desenvolvido por Lindholm e colaboradores há mais de duas décadas, tem uma deficiência. As energias de excitação calculadas por HAM/3 para estados degenerados são desdobradas. Recentemente foi proposto um método para corrigir esta deficiência. Apresentamos aqui resultados de aplicações deste novo método para algumas moléculas com simetria Td, C3v e D3h. O esquema proposto apresenta a degenerescência correta para as moléculas estudadas.445449Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Possibility Of Simultaneous Observation Of Nucleus Fragment And -ray Family In The Stratosphere

We propose simultaneous observation of surviving fragment nuclei (,Li,...) and the -ray family (,e,N,...) produced by the collision of an ultrahigh-energy cosmic-ray nucleus (10141016 eV/nucleus) with an air target in the stratosphere (10 g/cm2). We support the proposal with extensive Monte Carlo calculations, which are also relevant to analysis of other atmospheric cascade experiments. © 1987 The American Physical Society.36378379