Laparoscopic Synchronous Resection for Descending Colon Cancer and Tailgut Cyst

A 67-year-old woman underwent polypectomy for a tumor at the descending colon. Pathologically, the tumor was diagnosed as adenocarcinoma with an invasion of 2000 μm. Computed tomography showed a swollen paracolic lymph node and a mass lesion in the presacral space. Magnetic resonance imaging revealed a multio-cular cystic lesion. On diagnosis of descending colon cancer and tailgut cyst, she underwent synchronous lapa-roscopic resection. Histopathologically, the colon cancer was diagnosed as pT1bN1M0, pStage IIIa. The pre-sacral cystic lesion was diagnosed as a nonmalignant tailgut cyst with negative surgical margin. The patient is currently doing well without recurrence at 28 months

The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses

Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed “The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research” based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci. They should be collected as soon as possible after resection: at the latest within about 3 h of storage at 4°C. Preferably, snap‐frozen samples should be stored in liquid nitrogen (about −180°C) until use. When intending to use genomic DNA extracted from formalin‐fixed paraffin‐embedded tissue, 10% neutral buffered formalin should be used. Insufficient fixation and overfixation must both be avoided. We hope that pathologists, clinicians, clinical laboratory technicians and biobank operators will come to master the handling of pathological tissue samples based on the standard operating procedures in these Guidelines to yield results that will assist in the realization of genomic medicine

Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero

Newly identified tissue-resident vascular precursor cells are recruited into growing vessels and contribute to vasculogenesis in adult mice

The Pacific lineage (2E) of JC polyomavirus is prevalent in Sumba Island, Eastern Indonesia

Recent studies have identified a Pacific lineage (2E) of JC polyomavirus (also designated as JC virus or JCV) that occurs in both Island Southeast Asia and Oceania, but not in mainland Asia. It has been postulated that this lineage traveled with Austronesian-speaking people who are now spread throughout Island Southeast Asia and Oceania (excluding Australia and inland and southern New Guinea). However, to date, the 2E lineage has been identified in Southeast Asia only in populations of the Philippine islands. Here we report that a high incidence of the 2E lineage was detected in the people of Sumba Island, eastern Indonesia

Transcriptional control region rearrangements associated with the evolution of JC polyomavirus

AbstractJC polyomavirus (JCV) isolates worldwide are classified into three super-lineages (A, B and C), with A and B further split into several lineages and sub-lineages. The transcriptional control region (TCR) of the JCV genome generally has the archetypal configuration, but rearranged TCRs have occasionally been detected in isolates from immunocompetent individuals. To investigate the phylogenetic significance of these rearrangements, we analyzed 298 TCR sequences all derived from complete JCV genomes directly cloned from the urine of non-immunocompromised individuals. While sporadic rearrangements were found in many lineages and sub-lineages, common rearrangements were identified in all, or essentially all, isolates belonging to particular lineages or sub-lineages. Interestingly, several common rearrangements were also detected as sporadic rearrangements in other lineages or sub-lineages. This observation suggests that during the course of JCV evolution, JCV strains with sporadic rearrangements became predominant over archetypal TCRs in some JCV lineages or sub-lineages

Occurrence of the European Subgroup of Subtype I BK Polyomavirus in Japanese-Americans Suggests Transmission outside the Family▿

To examine the mode of transmission of BK polyomavirus (BKV), urine samples were collected from Japanese-Americans in Los Angeles and from other southern Californians. Subtype I was the main subtype found in samples from both groups. The subtype I subgroup Ib-2, which is predominant in Europe, was the primary subgroup detected in second-generation Japanese-Americans and in southern Californians; however, the Ic subgroup prevalent in native Japanese was rare in these populations. Since the European subgroup (Ib-2) predominated in the studied geographic area, the findings demonstrate that transmission outside the family is common in the spread of BKV, unlike previous findings for JC polyomavirus

Rare-Earth(RE)–Barium Solubility Behaviour in Y(Ba<SUB>2&#8722;x</SUB>RE<SUB>x</SUB>)Cu<SUB>3</SUB>O<SUB>7+&#948;</SUB> and Sm(Ba<SUB>2&#8722;x</SUB>RE<SUB>x</SUB>)Cu<SUB>3</SUB>O<SUB>7+&#948;</SUB>

The lattice constants and the superconducting transition temperatures of the series Y(Ba2&#8722;xREx)Cu3O7+&#948;(RE=Pr, Eu, and Gd; x&#x2266;0.4) show that the solubility of RE with Ba is practically absent for Eu and Gd, whereas a small solubility of Pr cannot be ruled out. The degree of RE-Ba solubility limit in the Y-based systems is significantly smaller relative to that in the respective RE based series. This observation could be consistently understood by our earlier proposal on the RE-Ba solubility dependence on the degree of O-O repulsive interaction. Our results also indicate that Pr is trivalent in these oxides, contrary to a previous report

Absence of solid solution of the type, Y(Ba<SUB>2&#8722;x</SUB>RE<SUB>x</SUB>)Cu<SUB>3</SUB>O<SUB>7&#177;&#948;</SUB> and its possible implications

The results of X-ray diffraction and resistivity measurements of the specimens Y(Ba2&#8722;xREx)Cu3O7&#177;&#948; (RE=Ce, Pr, Eu and Gd; x\lesssim0.4) are presented. The results suggest that the RE ions are not soluble with Ba in this series. This indicates that RE-Ba ionic size differences alone cannot explain the solubility behaviour normally observed in REBa2Cu3O7&#177;&#948; compounds. We propose that O-O repulsive interaction plays a major role in deciding the solubility limits