Time-dependent energetic proton acceleration and scaling laws in ultra-intense laser pulses interactions with thin foils

A two-phase model, where the plasma expansion is an isothermal one when laser irradiates and a following adiabatic one after laser ends, has been proposed to predict the maximum energy of the proton beams induced in the ultra-intense laser-foil interactions. The hot-electron recirculation in the ultra-intense laser-solid interactions has been accounted in and described by the time-dependent hot-electron density continuously in this model. The dilution effect of electron density as electrons recirculate and spread laterally has been considered. With our model, the scaling laws of maximum ion energy have been achieved and the dependence of the scaling coefficients on laser intensity, pulse duration and target thickness have been obtained. Some interesting results have been predicted: the adiabatic expansion is an important process of the ion acceleration and cannot be neglected; the whole acceleration time is about 10-20 times of laser pulse duration; the larger the laser intensity, the more sensitive the maximum ion energy to the change of focus radius, and so on.Comment: 15 pages, 4 figures, submitted to PR

A snoRNA modulates mRNA 3' end processing and regulates the expression of a subset of mRNAs.

mRNA 3' end processing is an essential step in gene expression. It is well established that canonical eukaryotic pre-mRNA 3' processing is carried out within a macromolecular machinery consisting of dozens of trans-acting proteins. However, it is unknown whether RNAs play any role in this process. Unexpectedly, we found that a subset of small nucleolar RNAs (snoRNAs) are associated with the mammalian mRNA 3' processing complex. These snoRNAs primarily interact with Fip1, a component of cleavage and polyadenylation specificity factor (CPSF). We have functionally characterized one of these snoRNAs and our results demonstrated that the U/A-rich SNORD50A inhibits mRNA 3' processing by blocking the Fip1-poly(A) site (PAS) interaction. Consistently, SNORD50A depletion altered the Fip1-RNA interaction landscape and changed the alternative polyadenylation (APA) profiles and/or transcript levels of a subset of genes. Taken together, our data revealed a novel function for snoRNAs and provided the first evidence that non-coding RNAs may play an important role in regulating mRNA 3' processing

The 2-Pebbling Property of the Middle Graph of Fan Graphs

A pebbling move on a graph G consists of taking two pebbles off one vertex and placing one pebble on an adjacent vertex. The pebbling number of a connected graph G, denoted by f(G), is the least n such that any distribution of n pebbles on G allows one pebble to be moved to any specified but arbitrary vertex by a sequence of pebbling moves. This paper determines the pebbling numbers and the 2-pebbling property of the middle graph of fan graphs

Solving Gravity Anomaly Matching Problem Under Large Initial Errors in Gravity Aided Navigation by Using an Affine Transformation Based Artificial Bee Colony Algorithm

Gravity aided inertial navigation system (GAINS), which uses earth gravitational anomaly field for navigation, holds strong potential as an underwater navigation system. The gravity matching algorithm is one of the key factors in GAINS. Existing matching algorithms cannot guarantee the matching accuracy in the matching algorithms based gravity aided navigation when the initial errors are large. Evolutionary algorithms, which are mostly have the ability of global optimality and fast convergence, can be used to solve the gravity matching problem under large initial errors. However, simply applying evolutionary algorithms to GAINS may lead to false matching. Therefore, in order to deal with the underwater gravity matching problem, it is necessary to improve the traditional evolutionary algorithms. In this paper, an affine transformation based artificial bee colony (ABC) algorithm, which can greatly improve the positioning precision under large initial errors condition, is developed. The proposed algorithm introduces affine transformation to both initialization process and evolutionary process of ABC algorithm. The single-point matching strategy is replaced by the strategy of matching a sequence of several consecutive position vectors. In addition, several constraints are introduced to the process of evolution by using the output characteristics of the inertial navigation system (INS). Simulations based on the actual gravity anomaly base map have been performed for the validation of the proposed algorithm

Mechanistic examination of causes for narrow distribution in an endangered shrub: a comparison of its responses to drought stress with a widespread congeneric species

Although deep rooting is usually considered a drought-tolerant trait, we found that Syringapinnatifolia, a deep rooting and hydrotropic shrub, has a limited distribution in arid areas. To elucidate the mechanisms for its narrow distribution, we conducted two experiments to examine the physiological and morphological responses to water availability and heterogeneity in S. pinnatifolia and a widespread congeneric species, S. oblata. We measured gas exchange, water use efficiency, and plasticity index in plants of these two species grown at different levels of soil water regimes and in containers with patched water distribution. Our results showed that high photosynthetic capacity in the narrowly distributed S. pinnatifolia was an important factor enabling its survival in the harsh sub-alpine environment. High photosynthetic capacity in S. pinnatifolia, however, was obtained at the expense of high transpiratory water loss, resulting in lower integrative water use efficiency. Biomass allocation to roots in S. pinnatifolia increased by 73 % when soil water increased from 75 to 95 % field capacity, suggesting that S. pinnatifolia could be less competitive for above-ground resources under favorable water regimes. The horizontal root hydrotropism and vertical root hydrotropism of S. pinnatifolia in soil with patched water patterns were likely related to compensation for leaf water loss at low soil water level, indicating a limited capacity for homeostasis within the plant for water conservation and lower level of inherent drought-tolerance. In summary, greater degree of morphological plasticity but lower degree of physiological adjustment may be the main causes for the hydrotropism and narrow distribution of S. pinnatifolia in the sub-alpine habitats

Identification of novel driver mutations of the discoidin domain receptor 2 (DDR2) gene in squamous cell lung cancer of Chinese patients

BACKGROUND: Although many of the recently approved genomically targeted therapies have improved outcomes for patients in non–small-cell lung cancer (NSCLC) with lung adenocarcinoma, little is known about the genomic alterations that drive lung squamous cell cancer (SCC) and development of effective targeted therapies in lung SCC is a promising area to be further investigated. Discoidin domain receptor 2 (DDR2), is a novel receptor tyrosine kinases that respond to several collagens and involved in tissue repair, primary and metastatic cancer progression. METHODS: Expression of DDR2 mRNA was analyzed in 54 lung SCC tissues by qRT-PCR. Over-expression approaches were used to investigate the biological functions of DDR2 and its’ mutations in lung SCC cells. Conventional Sanger sequencing was used to investigate the mutations of DDR2 gene in 86 samples. The effect of DDR2 and its’ mutations on proliferation was evaluated by MTT and colony formation assays; cell migration and invasion was evaluated by trasnwell assays. Lung SCC cells stably transfected with pEGFP-DDR2 WT, pEGFP-DDR2-S131C or empty vector were injection into nude mice to study the effect of DDR2 and its’ mutation on tumorigenesis in vivo. Protein and mRNA expression levels of E-cadherin and MMP2 were determined by qRT-PCR and western blot analysis. Differences between groups were tested for significance using Student’s t-test (two-tailed). RESULTS: In this study, we found that DDR2 mRNA levels were significantly decreased in 54 lung SCC tissues compared with normal lung tissues. Moreover, there were 3 novel DDR2 mutations (G531V, S131C, T681I) in 4 patients and provide the mutation rate of 4.6% in the 86 patients with lung SCC. The mutation of S131C in DDR2 could promote lung SCC cells proliferation, migration and invasion via inducing MMP-2, but reducing E-cadherin expression. CONCLUSIONS: These data indicated that the novel DDR2 mutation may contribute to the development and progression of lung SCC and this effect may be associated with increased proliferation and invasiveness, at least in part, via regulating E-cadherin expression

Petrogenesis of Himalayan leucogranites: Perspective from a Combined Elemental and Fe‐Sr‐Nd isotope study

The petrogenesis of Himalayan leucogranites remains crucial for understanding the thermal and tectonic evolution of the Himalayan orogen. To understand whether they are largely pristine melts of crustal anatexis or have experienced a high degree of fractional crystallization (FC), we present Fe isotopic data of 30 representative Himalayan leucogranites and 9 local metasedimentary rocks. Excepting three garnet leucogranites with low δ56Fe (−0.04‰–0.06‰) that are likely affected by garnet accumulation, tourmaline, and two-mica leucogranites have largely homogeneous δ56Fe from 0.13‰ to 0.24‰ irrespective of their highly variable SiO2, MgO, and FeOt contents. Combined with observed mineral assemblages and available fractionation factors, this does not support a high degree of FC (with or without assimilation) in their petrogenesis. The elevated δ56Fe relative to the supposed source rocks, represented by metasedimentary rocks and/or metabasite with a δ56Fe value of 0.10‰, by ∼0.07‰, may reflect Fe isotope fractionation during crustal anatexis. This study indicates most leucogranites can provide robust constraints on the conditions of crustal anatexis and thus the thermal and tectonic evolution of the Himalayan orogen

Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27

Infection by viruses, including herpes simplex virus-1 (HSV-1), and cellular stresses cause widespread disruption of transcription termination (DoTT) of RNA polymerase II (RNAPII) in host genes. However, the underlying mechanisms remain unclear. Here, we demonstrate that the HSV-1 immediate early protein ICP27 induces DoTT by directly binding to the essential mRNA 3' processing factor CPSF. It thereby induces the assembly of a dead-end 3' processing complex, blocking mRNA 3' cleavage. Remarkably, ICP27 also acts as a sequence-dependent activator of mRNA 3' processing for viral and a subset of host transcripts. Our results unravel a bimodal activity of ICP27 that plays a key role in HSV-1-induced host shutoff and identify CPSF as an important factor that mediates regulation of transcription termination. These findings have broad implications for understanding the regulation of transcription termination by other viruses, cellular stress and cancer