A BIO-ELECTRICAL MODEL FOR PHYSIOLOGICAL EVALUATION OF NADI PARIKSHA (AYURVEDIC PULSE DIAGNOSIS)

Physiology of the traditional Ayurvedic diagnostic technique of Nadipariksha has always been a matter of controversy. This diagnostic skill is based on a fine tactile sensitivity of the physician to distinguish varied intensities of mechanical vibrations elicited by radial artery pulsation, normally perceived as Pulse Pattern Variability (PPV). Ancient exponents of Yoga and Ayurveda define Nadi as the channel of Prana which is known as the very basis of bio-energy dynamics of the body. To the modern physician Nadipariksha indicates an assessment of cardiac function which is an important aspect of vascular physiology. The above concept has been re-examined in this article, in the light of whole body bio-electrical activity pattern. The study suggests Nadi as the Energy Pulsating Pathway (EPP) encompassing cells Pulsating Bio-Electrical Response (PBER). The observed varying intensities of PPV in Nadipariksha are proposed to be due to collision of weak threshold PBER with the mechanical vibration of pulsating radial artery. The underlying mechanism of Nadipariksha has been proposed to be associated with energy dynamics of bio-electrical waveform activity at the cellular level. This approach points at its possible implications in developing a more objective diagnostic method in assessing psycho-physiological abnormalities of a patient without having to depend on the subjective diagnostic judgment of a physician. Further, this study hints at the possible experimental evidence towards a physiological evaluation of Nadipariksha through the measures of bio-impedance, bio-reactance and bio-phase angle

Myxobacterial diversity of Indian soils - How many species do we have?

Myxobacteria of tropical soils is an under-explored bacterial group. We report below the results of sampling in Pune district of Western Ghats. A number of novel morphotypes were found in forest as well as urban/semi-urban soils. There was a high level of floral dissimilarity between habitats. The morphotypes detected in Pune district also differed from the northern Indian species recorded earlier. Using a species individual curve on the Pune, Lucknow and pooled data, we try to estimate the number of species that are likely to be present in India. A plausible estimate is several fold higher than the species recorded worldwide so far

Detection, Isolation and Confirmation of Crimean-Congo Hemorrhagic Fever Virus in Human, Ticks and Animals in Ahmadabad, India, 2010–2011

A nosocomial outbreak of CCHFV occurred in January 2011, in a tertiary care hospital in Ahmadabad, Gujarat State in western India. Out of a total five cases reported, contact transmission occurred to three treating medical professionals, all of whom succumbed to the disease. The only survivor was the husband of the index case. These results highlight the importance of considering CCHFV as a potential aetiology for Hemorrhagic fever (HF) cases in India. This also underlines the need for strict barrier nursing and patient isolation while managing these patients. During the investigation presence of CCHFV RNA in Hyalomma anatolicum ticks and livestock were detected in the village from where the primary case (case A) was reported. Further retrospective investigation confirmed two CCHF human cases in Rajkot village 20 kilometres to the west of Ahmadabad in 2010, and CCHFV presence in the livestock 200 kilometres to the north in the neighbouring State Rajasthan. This report shows the presence of CCHFV in human, ticks and animals in Gujarat, India. The fact of concern is the spread of this disease from one state to another due to trading of livestock

E-Graphologist for Personality Profile

Deep learning of a person’s signature can revealhis personality profile like fear, honesty, emotional state, thinkingstyle and much more. Analyzing signature can help in predictingsocial skills, thinking style, work habits, achievements, etc. of thewriter. Type and style of signature can be judged by graphologistbased on curved start, single line, dot on letter, etc. Similarlypattern recognition and image processing are used to analyzesignature and handwriting in our system. Here the signature isconsidered as image and then prediction is performed throughdifferent stages such as gray level conversion, calculatingthreshold value, binary conversion etc. The most preferredtechnique by researchers for personality prediction is 'ArtificialNeural Network'

Long-term effect of ancient Ayurvedic Agnikarma therapy on heel pain associated with calcaneal spur: A case report

We report the therapeutic effect of ancient Ayurvedic Agnikarma therapy on severe heel pain due to calcaneal spur. The patient, female, 34 years of age, presented with localized, severe and intermittent heel pain which increased with physical activities such as walking or running. A lateral X-ray revealed a large, well-defined bony projection at the base of calcaneus in both the heels. Agnikarma is an ancient Indian Ayurvedic surgical treatment for pain associated with ligament, joints and bones. It involves first- or second-degree burn (thermal cauterization) delivered precisely to the site of pain by using a specialized tool namely Shalaka, made up of five metals. Agnikarma provides instant, long-term and sustainable relief from chronic or acute pain by balancing the local vata and kapha doshas without any side effects. In the present case, Agnikarma treatment was administered for three consecutive weeks and the heel pain was rated on the Pain Analog Scale at each follow-up for up to three years. The patient reported a decrease in pain at each follow-up. No concomitant treatment was given for heel pain during the follow-up period. Agnikarma can have incredible benefits in the management of acute or chronic disorders of joints, ligaments and bones. Further, Agnikarma therapy is cost-effective and does not require hospitalization of the patient

A qualitative IgG ELISA for detection of SARS-CoV-2-specific antibodies in Syrian hamster serum samples

Summary: This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of inactivated virus antigens and the negative control antigen and the use of antigen-coated microtiter plates to detect SARS-CoV-2-specific antibodies from SARS-CoV-2-infected hamsters, including the criteria for differentiating positive versus negative reaction. The limited batch-to-batch variability of this assay has been verified with two batches of independently prepared antigens.For complete details on the use and execution of this protocol, please refer to Mohandas et al. (2021)

Sero-prevalence of Nipah antibodies among close contacts of the index case during 2019 Ernakulam outbreak

Introduction: Nipah virus (NiV) infection is a fatal emerging zoonotic disease. Infection with NiV has a wide range of clinical spectrum which can range from asymptomatic cases to acute respiratory distress syndrome (ARDS). The index case of NiV infection of 2019 outbreak in Ernakulam district was a 23-year-old male who presented with features of encephalitis. This study was undertaken to address the subclinical or asymptomatic NiV infection amongst the close contacts of this index case by using NiV-specific Immunoglobulin IgM and IgG antibodies. The index case was first treated in a primary care center. He survived the infection and was discharged after a period of 108 days from the tertiary care facility where he was treated eventually. Methods: Serum samples from 49 close contacts of the index case were collected and tested for anti-NiVIgM and anti-NiVIgG antibodies. The contacts included health care workers including those from the primary care facility, family members, and his friends. Results: Most common type of exposure included physical contact (59.2%), followed by exposure to body fluids (22.4%). Conclusion: None of the 49 contacts tested positive for anti-NiV human IgM and anti-NiVIgG antibodies. There were no subclinical cases amongst the close contacts of Nipah index case during the 2019 Kerala outbreak

Genomic characterization, transcriptome analysis, and pathogenicity of the Nipah virus (Indian isolate)

ABSTRACTNipah virus (NiV) is a high-risk pathogen which can cause fatal infections in humans. The Indian isolate from the 2018 outbreak in the Kerala state of India showed ~ 4% nucleotide and amino acid difference in comparison to the Bangladesh strains of NiV and the substitutions observed were mostly not present in the region of any functional significance except for the phosphoprotein gene. The differential expression of viral genes was observed following infection in Vero (ATCC® CCL−81™) and BHK−21 cells. Intraperitoneal infection in the 10–12-week-old, Syrian hamster model induced dose dependant multisystemic disease characterized by prominent vascular lesions in lungs, brain, kidney and extra vascular lesions in brain and lungs. Congestion, haemorrhages, inflammatory cell infiltration, thrombosis and rarely endothelial syncitial cell formation were seen in the blood vessels. Intranasal infection resulted in respiratory tract infection characterised by pneumonia. The model showed disease characteristics resembling the human NiV infection except that of myocarditis similar to that reported by NiV-Malaysia and NiV-Bangladesh isolates in hamster model. The variation observed in the genome of the Indian isolate at the amino acid levels should be explored further for any functional significance

A mini-review of Bunyaviruses recorded in India

Newly emerging and re-emerging viral infections are of major public health concern. Bunyaviridae family of viruses comprises a large group of animal viruses. Clinical symptoms exhibited by persons infected by viruses belonging to this family vary from mild-to-severe diseases i.e., febrile illness, encephalitis, haemorrhagic fever and acute respiratory illness. Several arthropods-borne viruses have been discovered and classified at serological level in India in the past. Some of these are highly pathogenic as the recent emergence and spread of Crimean-Congo haemorrhagic fever virus and presence of antibodies against Hantavirus in humans in India have provided evidences that it may become one of the emerging diseases in this country. For many of the discovered viruses, we still need to study their relevance to human and animal health. Chittoor virus, a variant of Batai virus; Ganjam virus, an Asian variant of Nairobi sheep disease virus; tick-borne viruses such as Bhanja, Palma and mosquito-borne viruses such as Sathuperi, Thimiri, Umbre and Ingwavuma viruses have been identified as the members of this family. As Bunyaviruses are three segmented RNA viruses, they can reassort the segments into genetically distinct viruses in target cells. This ability is believed to play a major role in evolution, pathogenesis and epidemiology of the viruses. Here, we provide a comprehensive overview of discovery, emergence and distribution of Bunyaviruses in India

Biorisk assessment for infrastructure & biosafety requirements for the laboratories providing coronavirus SARS-CoV-2/(COVID-19) diagnosis

Novel coronavirus infection [coronavirus disease 2019 (COVID-19)] has spread to more than 203 countries of various regions including Africa, America, Europe, South East Asia and Western Pacific. The WHO had declared COVID-19 as the global public health emergency and subsequently as pandemic because of its worldwide spread. It is now one of the top-priority pathogens to be dealt with, because of high transmissibility, severe illness and associated mortality, wide geographical spread, lack of control measures with knowledge gaps in veterinary and human epidemiology, immunity and pathogenesis. The quick detection of cases and isolating them has become critical to contain it. To meet the increasing demand of the diagnostic services, it is necessary to enhance and expand laboratory capabilities since existing laboratories cannot meet the emerging demand. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a BSL-2 (Biosafety Level 2) agent and needs to be handled in biosafety cabinet using standard precautions. This review highlights minimum requirements for the diagnostic laboratories opting testing of material for the diagnosis of COVID-19 and associated biorisk to the individuals and to the community