145 research outputs found
Functional Assessment of the Medicago truncatula
The Medicago truncatula NIP/LATD (for Numerous Infections and Polyphenolics/Lateral root-organ Defective) gene encodes a protein found in a clade of nitrate transporters within the large NRT1(PTR) family that also encodes transporters of dipeptides and tripeptides, dicarboxylates, auxin, and abscisic acid. Of the NRT1(PTR) members known to transport nitrate, most are low-affinity transporters. Here, we show that M. truncatula nip/latd mutants are more defective in their lateral root responses to nitrate provided at low (250 μm) concentrations than at higher (5 mm) concentrations; however, nitrate uptake experiments showed no discernible differences in uptake in the mutants. Heterologous expression experiments showed that MtNIP/LATD encodes a nitrate transporter: expression in Xenopus laevis oocytes conferred upon the oocytes the ability to take up nitrate from the medium with high affinity, and expression of MtNIP/LATD in an Arabidopsis chl1(nrt1.1) mutant rescued the chlorate susceptibility phenotype. X. laevis oocytes expressing mutant Mtnip-1 and Mtlatd were unable to take up nitrate from the medium, but oocytes expressing the less severe Mtnip-3 allele were proficient in nitrate transport. M. truncatula nip/latd mutants have pleiotropic defects in nodulation and root architecture. Expression of the Arabidopsis NRT1.1 gene in mutant Mtnip-1 roots partially rescued Mtnip-1 for root architecture defects but not for nodulation defects. This suggests that the spectrum of activities inherent in AtNRT1.1 is different from that possessed by MtNIP/LATD, but it could also reflect stability differences of each protein in M. truncatula. Collectively, the data show that MtNIP/LATD is a high-affinity nitrate transporter and suggest that it could have another function
Identification of a New Pea Gene, PsNlec1, Encoding a Lectin-like Glycoprotein Isolated from the Symbiosomes of Root Nodules
Allelic differences in Medicago truncatula NIP/LATD mutants correlate with their encoded proteins’ transport activities in planta
This article examines the hypothesis that MtNIP/LATD may have another biochemical activity
A Peanut Nodule Lectin in Infected Cells and in Vacuoles and the Extracellular Matrix of Nodule Parenchyma
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Rapid Analysis of Legume Root Nodule Development Using Confocal Microscopy
This article discusses the rapid analysis of legume root nodule development using confocal microscopy
Functional Assessment of the Medicago truncatula NIP/LATD Protein Demonstrates That It Is a High-Affinity Nitrate Transporter
Article on the functional assessment of the Medicago truncatula NIP/LATD protein demonstrating that it is a high-affinity nitrate transporter
Endoplasmic Microtubules Configure the Subapical Cytoplasm and Are Required for Fast Growth of Medicago truncatula
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A Putative Transporter is Essential for Integrating Nutrient and Hormone Signaling with Lateral Root Growth and Nodule Development in Medicago truncatula
This article discusses a putative transporter for integrating nutrient and hormone signaling with lateral root growth and nodule development in Medicago truncatula
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