21 research outputs found
Analisis Portofolio Optimal Dengan Single Index Model Untuk Meminimumkan Risiko Bagi Investor Di Bursa Efek Indonesia (Studi Pada Saham Indeks Kompas 100 Periode Februari 2010-juli 2014)
Investments can be made in the capital market, capital market instruments which are mostly attractive for investors is stock. Stock provides a return in the form of capital gains and dividends yield, not only noticing the return, investors need to pay attention to the investments risk. Unsystematis risk can be minimized by forming the optimal portfolio using one of the methods that is single index model. Study purpose is to knowing the stocks forming the optimal portfolio, the proportion of funds allocated to each stocks, the level of expectation return and risk.The method used in this research is descriptive research method with a quantitative approach. The samples used were 46 stocks in Kompas 100 Index, which meets the criteria for sampling. The results showed that 12 stocks of forming optimal portfolio, the stocks of which are UNVR, TRAM, MNCN, BHIT, JSMR, BMTR, GJTL, KLBF, AALI, CPIN, AKRA, and ASRI. Stock with highest proportion of funds is TRAM (23,52%), stock with lowest proportion of funds is AALI (0,62%). Portfolio which are formed will give return expectations by 3,05477% and carry the risk for about 0,1228%
Profiling B‑Type Natriuretic Peptide Cleavage Peptidoforms in Human Plasma by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry
B-type
Natriuretic Peptide (BNP) is a biologically active circulating
hormone. Plasma concentrations of BNP are routinely used in the diagnosis
of heart failure, and the intravenous infusion of recombinant BNP
can be used for heart failure treatment. Like many bioactive polypeptides,
multiple plasma enzymes are known to cleave circulating BNP, and as
part of the CVD-B/D-HPP mandate, we sought to develop a technique
capable of profiling these catabolic processes in plasma. We used
a neutral-coated capillary electrophoresis-electrospray ionization
(CESI) separation system coupled with high-resolution mass spectrometry
to profile the proteolysis of exogenous recombinant BNP<sub>1–32</sub> in plasma. Our method utilizes electrokinetic injection of minimally
processed plasma samples to simultaneously monitor the dynamic generation
and breakdown of at least five BNP peptidoforms in plasma. By integrating
multisegment injection, our method can produce a multipoint BNP proteolytic
profile for one sample within an hour. We envision applying this method
to assess the potential relation between plasma-based BNP proteolysis
and heart failure as well as a means of monitoring BNP bioavailability
after therapeutic infusion
Scatter plot of iTRAQ quantified log2 (protein ratio) and MRM quantified log2 (protein ratio).
<p>(A) iTRAQ versus MRM log2(12 DAP/6 DAP). (B) iTRAQ versus MRM log2(18 DAP/6 DAP). (C) iTRAQ versus MRM log2(24 DAP/6 DAP). (D) iTRAQ versus MRM log2(30 DAP/6 DAP).</p
Stress Responsive Proteins Are Actively Regulated during Rice (<i>Oryza sativa</i>) Embryogenesis as Indicated by Quantitative Proteomics Analysis
<div><p>Embryogenesis is the initial step in a plant’s life, and the molecular changes that occur during embryonic development are largely unknown. To explore the relevant molecular events, we used the isobaric tags for relative and absolute quantification (iTRAQ) coupled with the shotgun proteomics technique (iTRAQ/Shotgun) to study the proteomic changes of rice embryos during embryogenesis. For the first time, a total of 2 165 unique proteins were identified in rice embryos, and the abundances of 867 proteins were actively changed based on the statistical evaluation of the quantitative MS/MS signals. The quantitative data were then confirmed using multiple reactions monitoring (MRM) and were also supported by our previous study based on two-dimensional gel electrophoresis (2 DE). Using the proteome at 6 days after pollination (DAP) as a reference, cluster analysis of these differential proteins throughout rice embryogenesis revealed that 25% were up-regulated and 75% were down-regulated. Gene Ontology (GO) analysis implicated that most of the up-regulated proteins were functionally categorized as stress responsive, mainly including heat shock-, lipid transfer-, and reactive oxygen species-related proteins. The stress-responsive proteins were thus postulated to play an important role during seed maturation.</p></div
Cluster result of the significantly regulated proteins.
<p>(A) Cluster heatmap of significantly regulated proteins using the protein abundance information. The five columns from left to right are labeled 6, 12, 18, 24 and 30 DAP at the top of the heatmap. The left braces indicate the two groups classified by cluster analysis. (B) Color diagram of the heatmap.</p
Evaluation of three technical replicates.
<p>(A) Venn chart showing the overlap of the identified proteins from the three replications. (B) Accumulated frequency graph of the coefficient of variation.</p
Rice embryogenesis stages and embryo phenotypes for each stage.
<p>(A) Embryogenesis stages and sampling time settings. (B) Pattern of embryo length change during embryogenesis. (C) Pattern of embryo weight change during embryogenesis. (D) Newly germinated bud length of embryos during embryogenesis. For (B) (C) and (D), the error bars indicate the standard derivation.</p
Relative abundance of lipid transfer proteins during embryogenesis.
<p>The error bars indicate the standard derivation. 1 indicates the LTP with Locus ID LOC_Os07g11630.1; 2, LOC_Os03g02050.1; 3, LOC_Os12g02320.1; 4, LOC_Os11g40530.1; 5, LOC_Os08g03690.1; 6, LOC_Os05g40010.1; 7, LOC_Os01g60740.2; 8, LOC_Os11g02400.1; 9, LOC_Os10g36170.1.</p
Expansion of the Ion Library for Mining SWATH-MS Data through Fractionation Proteomics
The strategy of sequential window
acquisition of all theoretical
fragment ion spectra (SWATH) is emerging in the field of label-free
proteomics. A critical consideration for the processing of SWATH data
is the quality of the ion library (or mass spectrometric reference
map). As the availability of open spectral libraries that can be used
to process SWATH data is limited, most users currently create their
libraries in-house. Herein, we propose an approach to construct an
expanded ion library using the data-dependent acquisition (DDA) data
generated by fractionation proteomics. We identified three critical
elements for achieving a satisfactory ion library during the iterative
process of our ion library expansion, including a correction of the
retention times (RTs) gained from fractionation proteomics, appropriate
integrations of the fractionated proteomics into an ion library, and
assessments of the impact of the expanded ion libraries to data mining
in SWATH. Using a bacterial lysate as an evaluation material, we employed
sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
to fractionate the lysate proteins and constructed the expanded ion
library using the fractionation proteomics data. Compared with the
ion library built from the unfractionated proteomics, approximately
20% more peptides were extracted from the expanded ion library. The
extracted peptides, moreover, were acceptable for further quantitative
analysis
Correction to Quantitative Analysis of the Human AKR Family Members in Cancer Cell Lines Using the mTRAQ/MRM Approach
Correction to Quantitative Analysis of the Human AKR
Family Members in Cancer Cell Lines Using the mTRAQ/MRM Approac