Zigbee-Based Positioning System For Coal Miners

AbstractChina's present communication systems in coal mine can not effectively provide the information such as dynamic distribution of coal miners in work and production environment. Hence, rescue efforts are hard to be carried out in case of coal mine accident. To deal with this problem, the paper at first introduces the characteristics, structure and network construction method of ZigBee protocol. Then, by using the ZigBee technology based on IEEE802.15.4 criteria, the wireless communication network and the structure of ZigBee communication module for coal mine are studied and designed. At last, multilateration positioning algorithm is employed to realize the positioning of coal miners. Coal miner positioning system enters into computer management system via network, laying important actual significance to the production safety and emergent resuce of coal mine

Hydrochemical and Isotopic Characterization of the Impact of Water Diversion on Water in Drainage Channels, Groundwater, and Lake Ulansuhai in China

Lakes are important natural water reservoirs that connect other water bodies and play essential roles in water supply, ecological preservation, and climate regulation. Because of global climate change and human activities, many lakes worldwide are facing severe challenges, such as ecological degradation and reductions in their water storage, levels, surface areas, and quality. Water diversion into lakes is considered an effective measure to address these challenges and has attracted much attention. Water has been diverted into Lake Ulansuhai through drainage channels from the Yellow River since 2013. This shallow lake is located in arid northern China and is greatly affected by high salinity and eutrophication. The lake is the lowest area in the Hetao basin and is a sink for terrestrial water in this region. High salinity in lake water, drainage channels, and groundwater caused by NaCl is an ongoing problem; however, water diversion has played an important role in dilution. The main hydrochemical type in the lake water is Cl·HCO3–Na·Mg, while those in the drainage channels and the groundwater show more diversity because of spatial differences. The main source of water in the lake (52–60%) is that diverted through six drainage channels on the west bank, followed by meteoric precipitation (36–38%). Groundwater recharge to the lake is minimal (west bank: 2–7%, and east bank: 1–5%). Extensive evaporation occurs in the lake before the lake water is discharged into the Yellow River through a waste canal. The hydrochemical evolution and salinization of the lake are dominated by the six drainage channels, followed by evaporation from the lake surface. Thus, resolution of soil salinization in the Hetao irrigation area is key to addressing salinity issues in the lake. This study will be helpful for the planning of future water diversion and ecological restoration

An Integrated Analytical Approach for Screening Functional Post-Translational Modification Sites in Metabolic Enzymes

Post-translational modifications (PTMs) are pivotal in the orchestration of diverse physiological and pathological processes. Despite this, the identification of functional PTM sites within the vast amount of data remains challenging. Conventionally, those PTM sites are discerned through labor-intensive and time-consuming experiments. Here, we developed an integrated analytical approach for the identification of functional PTM sites on metabolic enzymes via a screening process. Through gene ontology (GO) analysis, we identified 269 enzymes with lysine 2-hydroxyisobutyrylation (Khib) from our proteomics data set of Escherichia coli. The first round of screening was performed based on the enzyme structures/predicted structures using the TM-score engineer, a tool designed to evaluate the impact of PTM on the protein structure. Subsequently, we examined the influence of Khib on the enzyme–substrate interactions through both static and dynamic analyses, molecular docking, and molecular dynamics simulation. Ultimately, we identified NfsB K181hib and ThiF K83hib as potential functional sites. This work has established a novel analytical approach for the identification of functional protein PTM sites, thereby contributing to the understanding of Khib functions

An Integrated Analytical Approach for Screening Functional Post-Translational Modification Sites in Metabolic Enzymes

Post-translational modifications (PTMs) are pivotal in the orchestration of diverse physiological and pathological processes. Despite this, the identification of functional PTM sites within the vast amount of data remains challenging. Conventionally, those PTM sites are discerned through labor-intensive and time-consuming experiments. Here, we developed an integrated analytical approach for the identification of functional PTM sites on metabolic enzymes via a screening process. Through gene ontology (GO) analysis, we identified 269 enzymes with lysine 2-hydroxyisobutyrylation (Khib) from our proteomics data set of Escherichia coli. The first round of screening was performed based on the enzyme structures/predicted structures using the TM-score engineer, a tool designed to evaluate the impact of PTM on the protein structure. Subsequently, we examined the influence of Khib on the enzyme–substrate interactions through both static and dynamic analyses, molecular docking, and molecular dynamics simulation. Ultimately, we identified NfsB K181hib and ThiF K83hib as potential functional sites. This work has established a novel analytical approach for the identification of functional protein PTM sites, thereby contributing to the understanding of Khib functions