Single nucleotide polymorphisms in the D-loop region of mitochondrial DNA is associated with colorectal cancer outcome

<p>Single nucleotide polymorphisms (SNPs) in the displacement loop (D-Loop) of mitochondrial DNA (mtDNA) has been identified for their association with the risk and outcome in many cancers. We have identified risk associated D-loop SNPs for colorectal cancer previously, in the present study, we evaluate their prognostic value for postoperative survival of colorectal cancer (CRC). The minor haplotype of nucleotides 16290T and frequent haplotype of nucleotide 16298T in the hypervariable segment 1 (HV1) region of the D-loop were identified for their association with high survival rate of CRC. After adjusted with COX proportional hazard model, the nucleotide site of 16290 was identified as independent predictor for CRC (RR, 0.379; 95% CI, 0.171–0.839; <i>p</i> = 0.017). In conclusion, SNPs in the mtDNA D-Loop were found to be valuable markers for colorectal cancer outcome evaluation.</p

Optimal plate design problem in steel production

In this paper, we address a new optimal plate design problem in steel production, in which slab selection is jointly considered. On the basis of the underlying features, the problem is formulated as a mixed integer nonlinear programming (MINLP) model with generalized disjunctive programming (GDP) constraints. A logic-based outer approximation (L-OA) algorithm is proposed to solve the problem. Specifically, a two-stage heuristic method is designed to initialize the L-OA algorithm. Numerical results are presented to demonstrate that the proposed L-OA algorithm and the heuristic method are effective and computationally efficient

Microarray-Based Analysis of Gene Expression in <i>Lycopersicon esculentum</i> Seedling Roots in Response to Cadmium, Chromium, Mercury, and Lead

The effects of heavy metals in agricultural soils have received special attention due to their potential for accumulation in crops, which can affect species at all trophic levels. Therefore, there is a critical need for reliable bioassays for assessing risk levels due to heavy metals in agricultural soil. In the present study, we used microarrays to investigate changes in gene expression of <i>Lycopersicon esculentum</i> in response to Cd-, Cr-, Hg-, or Pb-spiked soil. Exposure to ¹/₁₀ median lethal concentrations (LC₅₀) of Cd, Cr, Hg, or Pb for 7 days resulted in expression changes in 29 Cd-specific, 58 Cr-specific, 192 Hg-specific and 864 Pb-specific genes as determined by microarray analysis, whereas conventional morphological and physiological bioassays did not reveal any toxicant stresses. Hierarchical clustering analysis showed that the characteristic gene expression profiles induced by Cd, Cr, Hg, and Pb were distinct from not only the control but also one another. Furthermore, a total of three genes related to “ion transport” for Cd, 14 genes related to “external encapsulating structure organization”, “reproductive developmental process”, “lipid metabolic process” and “response to stimulus” for Cr, 11 genes related to “cellular metabolic process” and “cellular response to stimulus” for Hg, 78 genes related to 20 biological processes (e.g., DNA metabolic process, monosaccharide catabolic process, cell division) for Pb were identified and selected as their potential biomarkers. These findings demonstrated that microarray-based analysis of <i>Lycopersicon esculentum</i> was a sensitive tool for the early detection of potential toxicity of heavy metals in agricultural soil, as well as an effective tool for identifying the heavy metal-specific genes, which should be useful for assessing risk levels due to heavy metals in agricultural soil

Data_Sheet_1_The association between neuroendocrine/glucose metabolism and clinical outcomes and disease course in different clinical states of bipolar disorders.PDF

ObjectiveThe treatment of bipolar disorder (BD) remains challenging. The study evaluated the impact of the hypothalamic–pituitary–adrenal (HPA) axis/hypothalamic–pituitary-thyroid (HPT) axis and glucose metabolism on the clinical outcomes in patients with bipolar depression (BD-D) and manic bipolar (BD-M) disorders.MethodsThe research design involved a longitudinal prospective study. A total of 500 BD patients aged between 18 and 65 years treated in 15 hospitals located in Western China were enrolled in the study. The Young Mania Rating Scale (YMRS) and Montgomery and Asberg Depression Rating Scale (MADRS) were used to assess the BD symptoms. An effective treatment response was defined as a reduction in the symptom score of more than 25% after 12 weeks of treatment. The score of symptoms was correlated with the homeostatic model assessment of insulin resistance (HOMA-IR) index, the HPA axis hormone levels (adrenocorticotropic hormone (ACTH) and cortisol), and the HPT axis hormone levels (thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), free triiodothyronine (fT3), and free thyroxine (fT4)).ResultsIn the BD-M group, the YMRS was positively correlated with baseline T4 (r = 0.349, p = 0.010) and fT4 (r = 0.335, p = 0.013) and negatively correlated with fasting insulin (r = −0.289, p = 0.013). The pre-treatment HOMA-IR was significantly correlated with adverse course (p = 0.045, OR = 0.728). In the BD-D group, the baseline MADRS was significantly positively correlated with baseline fT3 (r = 0.223, p = 0.032) and fT4 (r = 0.315, p = 0.002), while baseline T3 (p = 0.032, OR = 5.071) was significantly positively related to treatment response.ConclusionThe HPT axis and glucose metabolism were closely associated with clinical outcomes at 12 weeks in both BD-D and BD-M groups. If confirmed in further longitudinal studies, monitoring T3 in BD-D patients and HOMA-IR for BD-M could be used as potential treatment response biomarkers.</p

Additional file 1 of Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes

Additional file 1: Fig. S1. Experimental verification of whether serum Exos can pass through the blood–brain barrier. Note: (A) Observation of neuron uptake of Exos by confocal fluorescence microscopy (400 ×); (B) RT-qPCR detection of MALAT1 and miR-382-3p expression in hippocampal neurons (P < 0.05 compared with the control + PBS group); C–E: RT-qPCR detection of MALAT1 and miR-382-3p expression in CPE, CSF, and brain parenchyma; (F) Observation of brain and hippocampal uptake of Exos by confocal fluorescence microscopy (20 × , 100 ×); (G) RT-qPCR detection of MALAT1 and miR-382-3p expression in mouse brain hippocampal tissue; n = 10, *, P < 0.05 compared with the PBS group, #, P < 0.05 compared with the Exos + DMSO group; all cell experiments were repeated three times

Additional file 3 of Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes

Additional file 3: Table S1. In vivo experimental grouping and treatments. Table S2. shRNA sequences. Table S3. In vitro cell experimental grouping and treatments. Table S4. The primer sequence of RT-qPCR. Table S5. Primary antibody product information. Table S6. T2DM-related lncRNAs obtained from the MNDR database

Additional file 2 of Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes

Additional file 2: Fig. S2. Schematic diagram illustrating the molecular mechanism of aerobic exercise improving cognitive impairment in T2DM mice by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes