Additional file 1 of Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes

Abstract

Additional file 1: Fig. S1. Experimental verification of whether serum Exos can pass through the blood–brain barrier. Note: (A) Observation of neuron uptake of Exos by confocal fluorescence microscopy (400 ×); (B) RT-qPCR detection of MALAT1 and miR-382-3p expression in hippocampal neurons (P < 0.05 compared with the control + PBS group); C–E: RT-qPCR detection of MALAT1 and miR-382-3p expression in CPE, CSF, and brain parenchyma; (F) Observation of brain and hippocampal uptake of Exos by confocal fluorescence microscopy (20 × , 100 ×); (G) RT-qPCR detection of MALAT1 and miR-382-3p expression in mouse brain hippocampal tissue; n = 10, *, P < 0.05 compared with the PBS group, #, P < 0.05 compared with the Exos + DMSO group; all cell experiments were repeated three times